ALL libraries (COBIB.SI union bibliographic/catalogue database)
13.
The role of selected mitochondrial proteins in neutrophil extracellular DNA trap formation = Vloga izbranih mitohondrijskih proteinov pri nastanku zunajceličnih DNA-pasti nevtrofilcev : Uniform Master's Study Programme Pharmacy
Fartelj, David
Neutrophils represent the majority of all immune cells and are arguably the most important cells of the innate immune system. Their response may be non-specific, but they are one of the first ...recognition sites against invading microorganisms. They can ingest a variety of pathogens by phagocytosis, destroying them in intracellular vesicles containing degrading enzymes and other antimicrobial substances. Besides to that, neutrophils possess a recently discovered mechanism to kill microbes extracellularly. During an infection, neutrophils release a structure with a DNA scaffold containing granule proteins called neutrophil extracellular traps (NETs). These structures are able to bind and kill pathogens. It is known that viable neutrophils can form NETs made up of mitochondrial DNA and granule proteins after short stimulation with GM-CSF and C5a. The goal of the thesis was to investigate the mechanism of NET formation using genetic and pharmacological approaches. We used genetically modified Hoxb8 mouse neutrophils and human neutrophils to study the molecular mechanisms of dsDNA release and NET formation. For the evaluation of the NETs, we used confocal laser scanning microscopy and PicoGreen dsDNA quantitation assay to quantify the released dsDNA in culture supernatants. Using Hoxb8 mouse and freshly isolated human neutrophils, we evaluated the importance of the pro-apoptotic proteins BAX and BAK, as well as the voltage-dependent anion-selective channel 1 (VDAC1) and Gasdermin D (GSDMD) for ROS production and NET formation. We demonstrate that both BAX and BAK may play a role in NET formation in the absence of cell death. We demonstrated that mouse neutrophils deficient in BAX or BAK exhibit reduced NET formation upon activation. In addition, using an inhibitor of voltage-dependent anion-selective channel 1 (VDAC1) oligomerization, we could determine that the formation of NETs does not require mitochondrial permeability transition pore (MPTP). Lastly, our results showed that GSDMD-dependent pyroptotic cell death does not play any role in mouse or human neutrophils NET formation.
Type of material - master's thesis
; adult, serious
Publication and manufacture - Ljubljana : [D. Fartelj], 2020
It was not necessary to add the material to the shelf.
Bibliographic material was successfully added on shelf.
Adding bibliographical material on shelf was not successful.
Material is already on the shelf.
Permalink
URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year
Impact factor
Edition
Category
Classification
JCR
SNIP
JCR
SNIP
JCR
SNIP
JCR
SNIP
Select the library membership card:
DRS,
in which the journal is indexed
Database name
Field
Year
Links to authors' personal bibliographies
Links to information on researchers in the SICRIS system
Subject headings in COBISS General List of Subject Headings
Select pickup location
The material from the parent unit is free. If the material is delivered to the pickup location from another unit, the library may charge you for this service.
