ALL libraries (COBIB.SI union bibliographic/catalogue database)
-
Flow cytometry analysis of ethanol induced cell death in cultured astrocytesLipnik-Štangelj, MetodaThe harmful effects of ethanol in the central nerve system result from direct toxic effects of ethanol as well as indirect effects, involving its metabolites and reactive oxygen substances (ROS). In ... the brain, astrocytes represent the major cellular localisation of ethanol metabolism; they are crucial in detoxification processes and in the maintenance of homeostasis. It has been found that ethanol can induce apoptosis in culture astrocytes. Since the astrocytes are relatively resistant to apoptosis, we were interested if ethanol is able to induce another form of programmed cell death - necroptosis. Cultured rat cortical astrocytes were used for cell death studies. A cell death was induced by incubation of the cells with different concentrations of ethanol for 24 h. After regeneration period, the cells were stained simultaneously with annexin V-FITC and 7-AAD dye. Different forms of cell death were detected using flow cytometry. We showed that acute exposure of cultured astrocytes to low concentrations (50 mM) of ethanol inhibits apoptosis. On the contrary, the number of necroptotic cells was significantly enhanced. The result is important, because the inhibition of apoptosis itself does not mean an inhibition of cell death. Distinction between different forms of regulated cell death is therefore crucial. In this sense, a flow cytometry analysis represents a simple in vitro approach for cell death studies.Source: Dokladi na B'lgarskata akademija na naukite = Comptes rendus de l'Académie Bulgare des sciences. - ISSN 1310-1331 (Vol. 66, no. 8, 2013, str. 1183-1190)Type of material - article, component part ; adult, seriousPublish date - 2013Language - englishCOBISS.SI-ID - 30774745
Author
Lipnik-Štangelj, Metoda
Topics
ethanol |
necroptosis |
apoptosis |
nekroptoza |
apoptoza
![loading ... loading ...](themes/default/img/ajax-loading.gif)
source: Dokladi na B'lgarskata akademija na naukite = Comptes rendus de l'Académie Bulgare des sciences. - ISSN 1310-1331 (Vol. 66, no. 8, 2013, str. 1183-1190)
![loading ... loading ...](themes/default/img/ajax-loading.gif)
![loading ... loading ...](themes/default/img/ajax-loading.gif)
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
If the library membership card is not in the list,
add a new one.
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|---|
Lipnik-Štangelj, Metoda | 15536 |
Source: Personal bibliographies
and: SICRIS
Select pickup location:
Material pickup by post
Delivery address:
Address is missing from the member's data.
The address retrieval service is currently unavailable, please try again.
By clicking the "OK" button, you will confirm the pickup location selected above and complete the reservation process.
By clicking the "OK" button, you will confirm the above pickup location and delivery address, and complete the reservation process.
By clicking the "OK" button, you will confirm the address selected above and complete the reservation process.
Notification
Automatic login and reservation service currently not available. You can reserve the material on the Biblos portal or try again here later.
Subject headings in COBISS General List of Subject Headings
Select pickup location
The material from the parent unit is free. If the material is delivered to the pickup location from another unit, the library may charge you for this service.
Pickup location | Material status | Reservation |
---|
Reservation in progress
Please wait a moment.
Reservation was successful.
Reservation failed.
Reservation...
Membership card:
Pickup location: