Akademska digitalna zbirka SLovenije - logo
ALL libraries (COBIB.SI union bibliographic/catalogue database)
PDF
  • Uroplakin traffic through the Golgi apparatus induces its fragmentation [Elektronski vir] : new insights from novel in vitro models
    Višnjar, Tanja, 1985- ...
    Uroplakins (UPs) play an essential role in maintaining an effective urothelial permeability barrier at the level of superficial urothelial cell (UC) layer. Although the organization of UPs in the ... apical plasma membrane (PM) of UCs is well known, their transport in UCs is only partially understood. Here, we dissected trafficking of UPs and its differentiation-dependent impact on Golgi apparatus (GA) architecture. We demonstrated that individual subunits UPIb and UPIIIa are capable of trafficking from the endoplasmic reticulum to the GA in UCs. Moreover, UPIb, UPIIIa or UPIb/UPIIIa expressing UCs revealed fragmentation and peripheral redistribution of Golgi-units. Notably, expression of UPIb or UPIb/UPIIIa triggered similar GA fragmentation in MDCK and HeLa cells that do not express UPs endogenously. The colocalization analysis of UPIb/UPIIIa-EGFP and COPI, COPII or clathrin suggested that UPs follow constitutively the post-Golgi route to the apical PM. Depolymerisation of microtubules leads to complete blockade of the UPIb/UPIIIa-EGFP post-Golgi transport, while disassembly of actin filaments shows significantly reduced delivery of UPIb/UPIIIa-EGFP to the PM. Our findings show the significant effect of the UPs expression on the GA fragmentation, which enables secretory Golgioutpost to be distributed as close as possible to the sites of cargo delivery at the PM.
    Source: Scientific reports [Elektronski vir]. - ISSN 2045-2322 (Vol. 7, Oct. 2017, str. 1-16)
    Type of material - e-article ; adult, serious
    Publish date - 2017
    Language - english
    COBISS.SI-ID - 33437401

    Link(s):

    https://www.nature.com/articles/s41598-017-13103-x
    DOI

Shelf entry