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Protocols and characterization data for 2 D, 3 D, and slice-based tumor models from the PREDECT projectHoogt, Ronald de ...Two-dimensional ( 2 D) culture of cancer cells in vitro does not recapitulate the three-dimensional ( 3 D) architecture, heterogeneity and complexity of human tumors. More representative models are ... required that better re fl ect key aspects of tumor biology. These are essential studies of cancer biology and immunology as well as for target validation and drug discovery. The Innovative Medicines Initiative (IMI) consortium PREDECT (www.predect.eu) characterized in vitro models of three solid tumor types with the goal to capture elements of tumor complexity and heterogeneity. 2 D culture and 3 D mono- and stromal co- cultures of increasing complexity, and precision-cut tumor slice models were established. Robust protocols for the generation of these platforms are described. Tissue microarrays were prepared from all the models, permitting immunohistochemical analysis of individual cells, capturing heterogeneity. 3 D cultures were also characterized using image analysis. Detailed step-by-step protocols, exemplary datasets from the 2 D, 3 D, and slice models, and re fi ned analytical methods were established and are presented.Source: Scientific data. - ISSN 2052-4463 (Vol. 4, Nov. 2017, str. 1-23)Type of material - e-article ; adult, seriousPublish date - 2017Language - englishCOBISS.SI-ID - 33698521
Author
Hoogt, Ronald de |
Estrada, Marta F. |
Vidic, Suzana, biologinja, 1978- |
Davies, Emma J. |
Osswald, Annika |
Barbier, Michaël |
Santo, Vitor E. |
Gjerde, Kjersti |
Zoggel, Hanneke J. A. A. van |
Blom, Sami
Topics
cancer |
tumor models |
protocols and characterization data |
rak |
tumorski modeli |
protokoli in podatki o karakterizaciji
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Hoogt, Ronald de | ![]() |
Estrada, Marta F. | ![]() |
Vidic, Suzana, biologinja, 1978- | 24444 |
Davies, Emma J. | ![]() |
Osswald, Annika | ![]() |
Barbier, Michaël | ![]() |
Santo, Vitor E. | ![]() |
Gjerde, Kjersti | ![]() |
Zoggel, Hanneke J. A. A. van | ![]() |
Blom, Sami | ![]() |
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