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Vpliv različnih virov ogljika na sestavo in reološke lastnosti zunajceličnih polisaharidov pri ocetnokislinskih bakterijah : magistrsko deloFijok, KimV magistrskem delu smo pri 83 sevih ocetnokislinskih bakterij (OKB) iz Zbirke mikroorganizmov Oddelka za biologijo Fakultete za naravoslovje in matematiko Univerze v Mariboru preiskali sposobnost za ... tvorbo zunajceličnih polisaharidov. 25 sevov je na gojišču RAE oblikovalo značilen biofilm. Štiri izbrane seve in še štiri referenčne seve smo vključili v nadaljnje analize. Z analizo PCR smo pri teh osmih sevih iskali specifične odseke ključnih genov za sintezo acetana (aceA), levana (levA) in celuloze (bscA). Pri enem sevu smo identificirali gen za sintezo acetana in levana, pri štirih gen za sintezo celuloze in acetana, pri dveh pa samo gen za sintezo celuloze. Pri enem izmed sevov nismo identificirali nobenega izmed treh iskanih genov, čeprav je sev v gojišču tvoril značilen biofilm. Po namnožitvi vsakega izmed teh sevov v dveh gojiščih, z različnima viroma ogljika, smo iz namnožene biomase izolirali vodotopne zunajcelične polisaharide. Očiščene polisaharide smo kislinsko hidrolizirali, jih ločili s tankoplastno kromatografijo ter identificirali po primerjavi s sladkornimi standardi. Zaradi drugačne monosaharidne sestave od že poznanega acetana in sposobnosti sevov, da producirajo vodotopne polisaharide v velikih količinah, smo izbrali tri seve za preiskovanje viskoelastičnih lastnosti polisaharidov. Najbolj viskozen je bil zunajcelični polisaharid pridobljen iz seva K. oboediens AV380. Bistveno nižjo viskoznost sta imela zunajcelična polisaharida, pridobljena iz sevov A. aceti LMG 1504T in A. pomorum LMG 18848T. Polisaharid seva K. oboediens AV380 je oblikoval viskoelastično trdno snov, zdržal najširše območje reverzibilnih deformacij in najkasneje razpadel, zato menimo, da ima potencialno uporabno vrednost v biotehnološki industriji in je primeren kandidat za nadaljnje raziskave.Type of material - master's thesis ; adult, seriousPublication and manufacture - Maribor : [K. Fijok], 2020Language - slovenianCOBISS.SI-ID - 34651139
Author
Fijok, Kim
Other authors
Trček, Janja |
Škraban, Jure |
Dogša, Iztok
Topics
magistrska dela |
ocetnokislinske bakterije |
biofilm |
zunajcelični polisaharidi |
acetan |
master theses |
acetic acid bacteria |
biofilm |
extracellular polysaccharides |
acetan
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Library/institution |
City | Acronym | For loan | Other holdings |
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Miklošič Library FPNM, Maribor | Maribor | PEFMB |
reading room 1 cop.
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Year | Impact factor | Edition | Category | Classification | ||||
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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DRS, in which the journal is indexed
Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Fijok, Kim | ![]() |
Trček, Janja | 14279 |
Škraban, Jure | 33271 |
Dogša, Iztok | 24407 |
Source: Personal bibliographies
and: SICRIS
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