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Izbira spermija v IVF laboratoriju = Selection of sperm in the IVF laboratory
Kovačič, Borut
Pri zdravljenju neplodnosti z zunajtelesno oploditvijo imata laboratorijska priprava semena in izbira semenčic za oploditev jajčnih celic zelo pomembno vlogo. Izbor semenčic vpliva na stopnjo ...oplojenosti, embrionalni razvoj in uspešnost postopka zdravljenja. Namen prispevka je prikazati številne laboratorijske metode, s katerimi se je iz semenskega izliva poskušalo izolirati najoptimalnejše semenčice. Opisane so prednosti in slabosti "swim-up" metode, ki jo najpogosteje uporabljamo pri klasični oploditvi in vitro. Naštete so tudi številne različice, ki so se sčasoma razvile iz te osnovne metode in so primerne v posebnih primerih, kot so težka oligoastenoterato- in kriptozoospermija. Opisane so tehnike za lažje iskanje in prepoznavanje živih semenčic iz vzorcev semena, kjer je gibljivost najbolj problematična. To so vzorci s popolnoma negibljivimi semenčicami in vzorci tkiva mod. Razvoj optične tehnologije je izboljšal možnosti izbora morfološko najoptimalnejših semenčic in tistih z nepoškodovano DNK.
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