-
Distribution of Photofrin between tumour cells and tumour associated macrophagesKorbelik, Mladen ...Photofrin levels in cells derived from SCCVII tumours, excised from mice that previously received the drug, were measured using a fluorescence activated cell sorter (FACS). Concomitantly, in the same ... cells the FACS was used to measure fluorescein isothiocyanate (FITC) fluorescence that originated from FITC-conjugated antimouse IgG added to the cell suspension before sorting. This later measurement enabled discrimination between IgG negative tumour malignant cells and IgG positive host cells (primarily macrophages). In addition, cellular Photofrin content in 'tumour' and 'host' cells sorted by FACS was determined by chemical extraction. The measurements were performed for the time intervals 1-96 h post Photofrin administration. The data showed consistently higher Photofrin levels in the 'host cells', i.e., tumour associated macrophages (TAM), than in 'tumour' cells. On a per cell basis, at any time point studied there was a minimum of 1.7 times more Photofrin in 'host' than in 'tumour cells', while at 4-12 h postadministration, ratios of up to 3.0 times were observed. This corresponds to ratio values greater than 9, when based on Photofrin content per micrograms cell protein.Source: British Journal of Cancer. - ISSN 0007-0920 (Letn. 64, št. 3, 1991, str. 508-512)Type of material - article, component partPublish date - 1991Language - englishCOBISS.SI-ID - 3507929
Author
Korbelik, Mladen |
Krošl, Gorazd |
Olive, P... L. |
Chaplin, D... J.
Topics
Carcinoma, squamous cell |
Metabolism |
Hematoporphyrins |
Pharmacokinetics |
Macrophages |
Metabolism |
Radiation-sensitizing agents |
Pharmacokinetics |
Carcinoma, squamous cell |
Pathology |
Cell adhesion |
Flow cytometry |
Methods |
Fluorescein-5-isothiocyanate |
Igg |
Analysis |
Macrophages |
Immunology |
Pathology |
Mice |
Mice, inbred c3h |
Time factors
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|---|
Korbelik, Mladen | 05280 |
Select pickup location:
Material pickup by post
Notification
Subject headings in COBISS General List of Subject Headings
Select pickup location
Pickup location | Material status | Reservation |
---|
Please wait a moment.