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  • Antibacterial activity of endophytic fungi isolated from conifer needles
    Ravnikar, Matjaž, farmacevt ...
    Fungi, in particular endophytes are a promising source of new antimicrobial compounds. The aim of this study was to screen the extracts of conifer needle fungal endophytes for antimicrobial activity ... and taxonomically place fungi producing ones to determined active metabolites. Seventy t hree strains of endophytic fungi were isolatedfrom plant samples, mainly from needles of conifers, and cultured. Extracts of cultured endophytic strains were tested for antimicrobial properties using a microdilution assay. Their activity was compared to t hat of the antibiotic ampicillin. Samples that exhibited antimicrobial properties were further examined. Genomic DNA from five active fungal strains was isolated and species - specific DNA regions (ITS regions) were amplified and sequenced allowing us to det ermine the identity of the samples. Active endophytic fungi were two strains of Lophodermium pinastri , two strains of Lophodermium seditiosum and one of Phoma herbarum . All of these strains are known as parasitic and can be treated as endophytes only accor ding to the lack of symptoms in their host tissue. This work demonstrates an interesting bottom - up approach tothe discovery of new antimicrobial compound.
    Source: African journal of biotechnology [Elektronski vir]. - ISSN 1684-5315 (Vol. 14, no. 10, 2015, str. 867-871)
    Type of material - article, component part ; adult, serious
    Publish date - 2015
    Language - english
    COBISS.SI-ID - 3909489

    Link(s):

    http://www.academicjournals.org/article/article1426245529_Ravnikar%20et%20al.pdf
    http://www.academicjournals.org/journal/AJB/article-abstract/572AE7551260
    DOI

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