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  • Expression of anti-cathepsin B human/murine chimeric antibody and its inhibitory effects on tumor cell invasion
    Xiaohui, Fan ...
    To prepare anti-human cathepsin B human-mouse chimeric antibody. Total RNA was extracted from hybridoma cell lines secreting monoclonal antibody against human cathepsin B, and the DNA fragments of ... monoclonal antibody VH and VL were amplified by RT-PCR, The chimeric light chain and heavy chain Fab antibody were ligated with the C%1 and C% constant region genes of human Ig by genetic engineering technique, and cloned The recombinant plasmid was transfected into Chinese hamster ovary cells (CHO). The anti-human cathepsin B human-mouse chimeric antibody was isolated and purified by G418 screening, ELISA and Western blot. (C62A2), secreted anti-human cathepsin B human-mouse chimeric antibody in vitro can inhibit cancer cells (C62A2), and the anti-human cathepsin B human-mouse chimeric antibody Conclusion The successful expression of human-mouse chimeric antibodies will likely be useful for the treatment of cathepsin B over-expressed disease-related diseases.
    Source: Zhonghua weishengwuxue he mianyixue zazhi = Chinese Journal of Microbiology and Immunology. - ISSN 0254-5101 (Vol. 23, iss. 11, 2003, str. 910-913)
    Type of material - article, component part
    Publish date - 2003
    Language - chinese
    COBISS.SI-ID - 4420721

    Link(s):

    http://eng.med.wanfangdata.com.cn/PaperDetail.aspx?qkid=zhwswxhmyx&qcode=zhwswxhmyx200311026
    http://med.wanfangdata.com.cn/Paper/Detail?id=PeriodicalPaper_zhwswxhmyx200311026

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