Priprava rekombinantnih človeških citokromov P450 in njihovo biokatalizno vrednotenje v sintezi metabolitov izbranih učinkovin; Production of recombinant human P450 cytochromes and their biocatalytic evaluation in selected drug metabolite synthesis : Master's Study Programme Industrial Pharmacy

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Priprava rekombinantnih človeških citokromov P450 in njihovo biokatalizno vrednotenje v sintezi metabolitov izbranih učinkovin = Production of recombinant human P450 cytochromes and their biocatalytic evaluation in selected drug metabolite synthesis : Master's Study Programme Industrial Pharmacy

Mihelčič, Kristina

V okviru magistrskega dela smo optimizirali izražanje več človeških paralogov CYP450 v metilotrofni kvasovki Pichia pastoris. Med gojenjem kvasovk smo spreminjali stopnjo aeracije, pH gojišča in ... hitrost mešanja. Prav tako smo primerjali tudi rast celic med gojenjem v bioreaktorjih in erlenmajericah ter aktivnost encimov v suspenziji celic. Katalitično aktivnost CYP450 smo ovrednotili z inkubacijo modelnih učinkovin testosterona in diklofenaka v kulturah rekombinantnih kvasovk, ekstrakcijo metabolitov in njihovo analizo s HPLC.

Type of material - master's thesis ; adult, serious

Publication and manufacture - Ljubljana : [K. Mihelčič], 2020

Language - english

COBISS.SI-ID - 45535491

Link(s):
https://repozitorij.uni-lj.si/IzpisGradiva.php?id=124054

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Other authors
Bratkovič, Tomaž, farmacevt, 1978- | Glieder, Anton

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Library/institution	City	Acronym	For loan	Other holdings
Faculty of Pharmacy, Ljubljana	Ljubljana	FFALJ	reading room 1 cop.

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Links to authors' personal bibliographies	Links to information on researchers in the SICRIS system
Mihelčič, Kristina
Bratkovič, Tomaž, farmacevt, 1978-	23598
Glieder, Anton

Source: Personal bibliographies and: SICRIS

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