ALL libraries (COBIB.SI union bibliographic/catalogue database)
-
Testing the potency of anti-TNF-[alphal] and anti-IL-[betal] drugs using spheroid cultures of human osteoarthritic chondrocytes and donor-matched chondrogenically differentiated mesenchymal stem cells [Elektronski vir]Žigon, Sara, farmacevtka ...Inflammation plays a major role in progression of rheumatoid arthritis, a disease treated with antagonists of tumor necrosis factor-alpha (TNF-alpha) and interleukin 1beta (IL-1beta). New in vitro ... testing systems are needed to evaluate efficacies of new anti-inflammatory biological drugs, ideally in a patient-specific manner. To address this need, we studied microspheroids containing 10,000 human osteoarthritic primary chondrocytes (OACs) or chondrogenically differentiated mesenchymal stem cells (MSCs), obtained from three donors. Hypothesizing that this system can recapitulate clinically observed effects of anti-inflammatory drugs, spheroids were exposed to TNF-alpha, IL-1beta, or to supernatant containing secretome from activated macrophages (MCM). The anti-inflammatory efficacies of anti-TNF-alpha biologicals adalimumab, infliximab, and etanercept, and the anti-IL-1beta agent anakinra were assessed in short-term microspheroid and long-term macrospheroid cultures (100,000 OACs). While gene and protein expressions were evaluated in microspheroids, diameters, amounts of DNA, glycosaminoglycans, and hydroxiproline were measured in macrospheroids. The tested drugs significantly decreased the inflammation induced by TNF-alpha or IL-1beta. The differences in potency of anti-TNF-alpha biologicals at 24 h and 3 weeks after their addition to inflamed spheroids were comparable, showing high predictability of short-term cultures. Moreover, the data obtained with microspheroids grown from OACs and chondrogenically differentiated MSCs were comparable, suggesting that MSCs could be used for this type of in vitro testing. We propose that in vitro gene expression measured after the first 24 h in cultures of chondrogenically differentiated MSCs can be used to determine the functionality of anti-TNF-alpha drugs in personalized and preclinical studies.Source: Biotechnology progress [Elektronski vir]. - ISSN 1520-6033 (Vol. 34, iss. 4, Jul/Aug 2018, str. 1045-1058)Type of material - e-articlePublish date - 2018Language - englishCOBISS.SI-ID - 4669809
Author
Žigon, Sara, farmacevtka |
Barlič, Ariana |
Knežević, Miomir |
Jeras, Matjaž |
Vunjak-Novakovic, Gordana
Topics
Matične celice |
Vnetje |
3D tissue spheroids |
anti-TNF-alpha biologicals |
human articular chondrocytes |
inflammation |
mesenchymal stem cells |
mezenhimske matične celice |
revmatoidni artritis
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
If the library membership card is not in the list,
add a new one.
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|---|
Žigon, Sara, farmacevtka | 35153 |
Barlič, Ariana | 15627 |
Knežević, Miomir | 10038 |
Jeras, Matjaž | 01302 |
Vunjak-Novakovic, Gordana |
Source: Personal bibliographies
and: SICRIS
Select pickup location:
Material pickup by post
Delivery address:
Address is missing from the member's data.
The address retrieval service is currently unavailable, please try again.
By clicking the "OK" button, you will confirm the pickup location selected above and complete the reservation process.
By clicking the "OK" button, you will confirm the above pickup location and delivery address, and complete the reservation process.
By clicking the "OK" button, you will confirm the address selected above and complete the reservation process.
Notification
Automatic login and reservation service currently not available. You can reserve the material on the Biblos portal or try again here later.
Subject headings in COBISS General List of Subject Headings
Select pickup location
The material from the parent unit is free. If the material is delivered to the pickup location from another unit, the library may charge you for this service.
Pickup location | Material status | Reservation |
---|
Reservation in progress
Please wait a moment.
Reservation was successful.
Reservation failed.
Reservation...
Membership card:
Pickup location: