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Coupling purification and on-column PEGylation of tumor necrosis factor alpha analogue
Milunović, Tatjana ...
Trends in preparation of PEGylated protein drugs strive for simple, fast and cheap processes, resulting in well-defined homogenous products. We investigated the on-column PEGylation of tumor necrosis ...alpha (TNF-Ž), where purification and conjugation were performed in one step by using Immobilized Metal Affinity Chromatography (IMAC). The same quality of the PEGylated product was obtained by the on-column approach starting from either the crude E. coli protein extract or the purified protein. In comparison to the PEGylation in solution the on-column approach resulted in more homogeneous PEGylated product. The on-column PEGylation reduces the number of production steps, expenses and preparation time
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