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Lubadarjem pri uspešnem napadu dreves pomagajo citokromi P450 simbiontskih gliv [Elektronski vir]Novak, Metka, biokemija, 1985-Gozd, ki je slabo prilagojen hitrim okoljskim spremembam, je še posebno dovzeten za izbruhe lubadarja, ki je sicer pomemben del gozdnega ekosistema. K napadu in naselitvi hrošča pripomorejo patogene ... glive modrivke, ki oslabijo in razstrupljajo obrambne spojine smreke. Presnova in sposobnost razstrupljanja drevesnih obrambnih spojin naj bi omogočali glivi, da preživi v toksičnem mikrookolju pod lubjem. Razumevanje glivne obrambe skozi biokemijske, genomske in metabolomske študije bo omogočilo razumevanje vloge gliv v tej simbiozi, ter možnih vzrokov ekonomsko škodljivih izbruhov hroščev podlubnikov. Nedavno so objavili zaporedje genoma in transkriptoma severno-ameriške glive modrivke Grosmannie clavigere, ki je uničujoč patogen na borovcih (Pinus contorta). Iz transkriptomskih podatkov s terpenoidi induciranega micelija, je razvidno povišano izražanje nekaterih genov za citokrome P450 (krajše CYP oz. P450). Pri glivah citokromi P450 sodelujejo prištevilnih pretvorbah tujih in glivi-lastnih metabolitov, kot so steroidi, aromatski ogljikovodiki in druge majhne molekule. Na osnovi ekspresijskega profila smo heterologno, v bakteriji E. coli in kvasovki S. cerevisiae, izrazili določene citokrome P450. Da bi biokemijsko potrdili vlogo encimov pri razstrupljanju drevesnih obrambnih spojin, smo pripravili in vitro eksperiment, kjer smo za potrditev substratne specifičnosti kot substrat uporabili monoterepene. Z analizo metabolitov glive, tretirane s posameznimi monoterpeni, smo z uporabo plinske kromatografije sklopljene z masno spektrometrijo (GC-MS), določili posamezne intermediate v metabolnih poteh pretvorbe teh spojin.Source: Zbornik povzetkov [Elektronski vir] (Str. 9)Type of material - conference contributionPublish date - 2012Language - slovenianCOBISS.SI-ID - 5124890
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Novak, Metka, biokemija, 1985- | 32116 |
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