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Vpliv saharoze, sorbitola in temperature na in vitro rast in ohranjanje ozkolistnega koščca (Berula erecta) = Effects of sucrose, sorbitol and temperature on in vitro growth and storage of Berula erecta : magistrsko delo
Oblonšek, Sanja
V magistrskem delu smo raziskovali možnosti za hrambo ozkolistnega koščca (Berula erecta) v tkivni kulturi z upočasnjeno rastjo. Upočasnitev rasti smo poskušali doseči z nižanjem temperature in ...dodajanjem sladkorjev in sladkornih alkoholov na gojišče MS. Ugotavljali smo vpliv nizkih temperatur (13 °C in 4 °C), različnih kombinacij in koncentracij sorbitola (20, 40 g l-1) in saharoze (30, 40 g l-1) ter svetlobe na viabilnost, rast in barvila ozkolistnega koščca. Spremljali smo različne rastne parametre, vsebnost fotosinteznih barvil in antocianinov. Povišanje koncentracije saharoze ni zavrlo oziroma upočasnilo rasti. Pri 23 % 2 °C je dodatek 20 g l-1 sorbitola uspešno zavrl rast, dodatek 40 g l-1 sorbitola pa je po dveh mesecih kulture vodil v senescenco rastlin. Hramba Berule erecta pri 13 °C v nobenem tretmaju ni bila učinkovita. Kot najbolj učinkovit način za dolgoročno hrambo B. erecta z minimalno rastjo in visoko viabilnostjo se je izkazala hramba rastlin pri 4 °C v temi.
Type of material - master's thesis
; adult, serious
Publication and manufacture - Maribor : [S. Oblonšek], 2021
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