-
Crystal structure of RahU, an aegerolysin protein from the human pathogen Pseudomonas aeruginosa, and its interaction with membrane ceramide phosphorylethanolamine [Elektronski vir]Kočar, Eva, 1992- ...Aegerolysins are proteins produced by bacteria, fungi, plants and protozoa. The most studied fungal aegerolysins share a common property of interacting with membranes enriched with cholesterol in ... combination with either sphingomyelin or ceramide phosphorylethanolamine (CPE), major sphingolipids in the cell membranes of vertebrates and invertebrates, respectively. However, genome analyses show a particularly high frequency of aegerolysin genes in bacteria, including the pathogenic genera Pseudomonas and Vibrio; these are human pathogens of high clinical relevance and can thrive in a variety of other species. The knowledge on bacterial aegerolysin-lipid interactions is scarce. We show that Pseudomonas aeruginosa aegerolysin RahU interacts with CPE, but not with sphingomyelin-enriched artificial membranes, and that RahU interacts with the insect cell line producing CPE. We report crystal structures of RahU alone and in complex with tris(hydroxymethyl)aminomethane (Tris), which, like the phosphorylethanolamine head group of CPE, contains a primary amine. The RahU structures reveal that the two loops proximal to the amino terminus form a cavity that accommodates Tris, and that the flexibility of these two loops is important for this interaction. We show that Tris interferes with CPE-enriched membranes for binding to RahU, implying on the importance of the ligand cavity between the loops and its proximity in RahU membrane interaction. We further support this by studying the interaction of single amino acid substitution mutants of RahU with the CPE-enriched membranes. Our results thus represent a starting point for a better understanding of the role of P. aeruginosa RahU, and possibly other bacterial aegerolysins, in bacterial interactions with other organisms.Source: Scientific reports [Elektronski vir]. - ISSN 2045-2322 (Vol. 11, iss. [article no.] 6572, 2021, str. 1-12)Type of material - e-articlePublish date - 2021Language - englishCOBISS.SI-ID - 56737283
Author
Kočar, Eva, 1992- |
Lenarčič, Tea |
Hodnik, Vesna, 1979- |
Panevska, Anastasija |
Huang, Yunjie |
Bajc, Gregor, dipl. biol. |
Kostanjšek, Rok |
Naren, Anjaparavanda P. |
Maček, Peter |
Anderluh, Gregor, 1969- |
Sepčić, Kristina |
Podobnik, Marjetka |
Butala, Matej, biokemik, 1979-
Topics
aegerolysins |
crystal structure of RahU |
ceramide phosphorylethanolamine |
aegerolizini |
kristalna struktura RahU |
ceramid fosforiletanolamin
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|---|
Kočar, Eva, 1992- | 52386 |
Lenarčič, Tea | 36425 |
Hodnik, Vesna, 1979- | 29235 |
Panevska, Anastasija | 39090 |
Huang, Yunjie | |
Bajc, Gregor, dipl. biol. | 30435 |
Kostanjšek, Rok | 18749 |
Naren, Anjaparavanda P. | |
Maček, Peter | 06994 |
Anderluh, Gregor, 1969- | 15686 |
Sepčić, Kristina | 15328 |
Podobnik, Marjetka | 12048 |
Butala, Matej, biokemik, 1979- | 24290 |
Select pickup location:
Material pickup by post
Notification
Subject headings in COBISS General List of Subject Headings
Select pickup location
Pickup location | Material status | Reservation |
---|
Please wait a moment.