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Primerjava med biološkimi testi in eliso za določanje interferonskih protiteles in interferonov = Comparative analysis between biological tests and eia assay for determination of interferon and it's antibodies : diplomsko delo
Žen, Maja
V opravljenih testih smo določevali občutljivost transformiranih in netransformiranih celic za posamezno vrsto humanih in prašičjih interferonov.Rezultati so pokazali zelo močno proticelično in ...citotoksično delovanje le-teh. Prav tako smo proučevali podobnost med interferonom podobnimi beljakovinami in interferoni na transformiranih in netranormiranih celicah. Primerjava med biološkimi testi in testom ELISA, je pokazala razmerje med količino interferonov in protiferonskih teles določeno z ELISO in njihovo aktivnostjo določeno z biološkimi testi. Iz vseh opravljenih testov lahko povzamemo, da so različne vrste celic različno občutljive na delovanje interferonov. Večjo občutljivost kažejo humane transformirane celice kot pa netranformirane. Prav tako se je pokazalo, da humani interferon alfa deluje najbolj proticelično in citoksično. Poskusi opravljeni z elektroindukcijo humanih levkocitov pa so nam pokazali, da lahko z metodo elektroindukcije dobimo interferonom podobne beljakovine.
Type of material - undergraduate thesis
; adult, serious
Publication and manufacture - Ljubljana : [M. Žen], 1996
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