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  • Primerjava ultrahitrega in počasnega zamrzovanja mišjih zarodkov = Comparison of ultrarapid and slow freezing of mouse embryos
    Kovačič, Borut ; Vlaisavljević, Veljko
    Background. The aim of the study was to compare the ultrarapid method of embryo freezing and the technique of slow cooling by means of a portable computer-controlled device Freeze Control L863 ... (Cryologic, Australia). Methods. Mouse embryos of different preblastular stages were frozen, and their morphologic survival immediately after thawing as well as their capacity for further development under in vitro conditions were evaluated. The success of freezing procedures was expressed by the percentage of thawed embryos which had reached the blastocyst stage - actual survival. Results. Only 40.2 percent (51/127) of four-cell embryos (p less th. 0.001) and 78.4 percent (76/97) of eight-cell embryos (p less th. 0.025) actually survived ultrarapid freezing. Slow freezing proved successful in 76.9 percent (103/134) of four-cell embryos (p less th. 0.01) and in 72.4 percent (113/156) of eight-cell embryos (p less th. 0.001). Embryos having less than half of their blastomeres intact after thawing also developed to blastocysts. Among such damaged embryos, vitality was conserved in 47.4 percent (9/19) of ultrarapidly frozen embryos and in 44.1 percent (15/34) of slowly frozen embryos. Conclusions. After ultrarapid freezing, early embryonal stages with larger blastomeres show a poor survival rate. In slow cooling using the controlled freezing device, the size of blastomeres bears no effect on the success of the procedure and is therefore more appropriate for clinical application in human material.
    Source: Zdravniški vestnik : glasilo Slovenskega zdravniškega društva = Slovenian medical journal = journal of Slovenian Medical Association. - ISSN 1318-0347 (Letn. 64, št. 7/8, jul.-avg. 1995, str. 413-417)
    Type of material - article, component part ; adult, serious
    Publish date - 1995
    Language - slovenian
    COBISS.SI-ID - 68554240

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