Akademska digitalna zbirka SLovenije - logo
ALL libraries (COBIB.SI union bibliographic/catalogue database)
  • Escherichia coli CoIV plasmid pRK100: genetic organization, stability and conjugal transfer
    Ambrožič, Jerneja, biologinja ...
    Uropathogenic Escherichia coli strains express chromosomal and plasmidencoded virulence-associated factors such as specific adhesins, toxins and ironuptake systems. A CoIV plasmid (pRK100) of a ... uropathogenic strain and its host KS533 were studied. The host strain encodes the K1 capsule, and P and S fimbriae, but neither haemolysin nor the cytotoxic-necrotic factor CNF1, indicating thatthis strain does not harbour a larger pathogenicity island. A restriction map of pKR100 was constructed on the basis of hybridization experiments and nucleotide sequencing. pRK100 harbours CoIV, the conserved replication region RepFIB, the aerobactin-uptake system, a RepFIC replicon and additonally Colla as well as transposon Tn5431. The location of the RepFIC replicon was similar to that in plasmid F. CoIV plasmids and F thus share a region spanning more than half the length of plasmid F. Even though their replication and transfer regions are homologous, CoIV plasmids are found only in E. coli strains. Amongthe four other species tested, conjugal transfer of pRK100 was demonstrated, with low frequency, only to Klebsiella pneumoniae, suggesting that a natural barrier effectively bars transfer. In vitro stability of the plasmid with integration into the chromosome to ensure maintenance in the presence of an incompatible plasmid was demostrated.
    Source: Microbiology. - ISSN 1350-0872 (Let. 144, 1998, str. 343-352)
    Type of material - article, component part
    Publish date - 1998
    Language - english
    COBISS.SI-ID - 7113689

source: Microbiology. - ISSN 1350-0872 (Let. 144, 1998, str. 343-352)
loading ...
loading ...
loading ...

Shelf entry