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Our experience with the enhanced polymer one-step staining in frozen sectionsRott, Tomaž ; Velkavrh, DaneThe enhanced polymer one-step staining (EPOS) method is presented with minor modifications for intraoperative frozen sections. It can be applied in almost every department of pathology where there is ... a necessity, because no special equipment is required. The method is rapid and immunostaining can be performedin less than 15 min. Antibodies are available for more than 20 important tissue and tumor markers. Out of those, antibodies against human cytokeratin, leukocyte common antigen (LCA) and chromogranin A were introducedin routine work. The first 2 antibodies yield excellent results and are a big advantage in routine diagnostic work: the use of cytokeratin is useful for confirmation or exclusion of suspected tumor invasion, especially in cases of atypical hyperplastic lesions, for detection of spindle cell carcinomas, and the detection of lymph node micrometastases. LCA demonstrationis helpful in differentiating various round cell tumors, such as poorly differentiated carcinomas, sarcomas, malignant lymphomas, and evaluation of inflammatory response. Chromogranin A may detect neuroendocrine differentiation in the tumors.Source: Acta oto-laryngologica. Supplementum. - ISSN 0365-5237 (supl. 527, 1997, str. 114-115)Type of material - article, component partPublish date - 1997Language - englishCOBISS.SI-ID - 7565017
Author
Rott, Tomaž |
Velkavrh, Dane
Topics
Carcinoma, small cell |
Pathology |
Diagnosis |
Frozen sections |
Immunohistochemistry |
Methods |
Lung neoplasms |
Intraoperative period |
Staining |
Methods
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Rott, Tomaž | 07184 |
Velkavrh, Dane | 18362 |
Source: Personal bibliographies
and: SICRIS
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