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Študij vpliva beljakovine DegU bakterije Bacillus subtilis na biosintezo bacitracina pri bakteriji Bacillus licheniformis = The study of Bacillus suptilis protein DegU on bacitracin biosynthesis in Bacillus licheniformis
Grošel, Alenka
Peptidni antibiotiki, ki jih sintetizirajo mnogi mikroorganizmi, so strukturno izredno raznoliki. Med njimi je tudi bacitracin, proizvod bakterije Bacillus licheniformis. Teh peptidov ne kodirajo ...geni; njihova biosinteza poteka s pomočjo peptidnih sintetaz. Raven biosinteze bacitracina pozitivno korelira z ravnjo sinteze alkalne proteinaze. Naš namen je bil dvigniti raven sinteze poznih encimov in posledično s tem tudi raven biosinteze bacitracina. Celice bakterije B. licheniformis BA1 smo transformirali z genom degU bakterije B. subtilis, za katerega smo menili, da je globalni pozitivni regulator v procesih kompetence in sporulacije. Pričakovali smo, da bo povišana koncentracija beljakovine DegU zvišala raven biosinteye bacitracina. Klone rekombiniranih sevov smo analizirali in jim določili proteolizno aktivnost. Pri dveh klonih produkcijskega seva bakterije B. licheniformis BA1 smo ugotovili višjo raven proizvodnje peptidnega antibiotika bacitracina.
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