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Modeling of kinetics for the enzymatic hydrolysis of sunflower oil in a high-pressure reactor
Primožič, Mateja, 1975- ; Leitgeb, Maja ; Knez, Željko
This study presents a mathematical model for characterizing the Michaelis-Menten type of sunflower oil hydrolysis, catalyzed by the lipase preparation Lipolase 100T(Novozymes Ays, Bagsvaerd, ...Denmark). Supercritical carbon dioxide was used as a solvent for this reaction. Computer modeling of the kinetics was done for the enzymatic hydrolysis of sunflower oil in a high-pressure reactor using the MicroMath Scientistr program (MicroMath Research, St. Louis, MO). On the basis of the experimental results, we presumed the reaction was reversible. The suitability of the model was confirmed statistically with Studentćs t-test. Good agreement was found between the experimental and calculated values for the concentrations of oleicand linoleic acids.
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