BF, Dept. of Food Science and Technology, Lj. (BFZIV)
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Vpliv mutacij v genih, pomembnih za tvorbo biofilma na adhezijo in invazijo bakterij Campylobacter jejuni v celicah Caco-2 [Elektronski vir] : magistrsko deloDamijan, TjašaMikroaerofilne bakterije Campylobacter jejuni so kljub zahtevnim pogojem rasti zelo razširjeni mikroorganizmi in najpogostejši povzročitelji akutnega bakterijskega gastroenteritisa pri ljudeh po vsem ... svetu. Predpostavlja se, da ima pri preživetju v naravnem okolju pomembno vlogo tvorba biofilma. Prvi in ključni korak, potreben za uspešno tvorbo biofilma, je adhezija bakterij na ustrezno površino. V magistrskem delu je bila ovrednotena sposobnost adhezije in invazije bakterij Campylobacter jejuni na/v humane črevesne celice karcinogenega izvora Caco-2. V raziskavo so bili vključeni referenčni sev Campylobacter jejuni (divji sev NCTC 11168) in njegovi mutanti z mutacijami v genih chuA, csrA, kpsM, luxS, omp50, rrpA, rrpB, trpE in Cj1191c. Vpliv mutacij v izbranih genih na sposobnost adhezije in invazije bakterij Campylobacter jejuni je bil ovrednoten z metodo štetja kolonij (CFU/ml). Pred tem je bil ovrednoten vpliv mutantov na metabolno aktivnost celic Caco-2 s kolorimetričnim testom z uporabo reagenta MTT. Statistična analiza je pokazala, da so mutacije v genih chuA, csrA, luxS, omp50 in rrpB statistično značilno zmanjšale sposobnost adhezije bakterij Campylobacter jejuni na celice Caco-2. Mutacije v genih kpsM, trpE in Cj1191c so statistično značilno povečale sposobnost adhezije bakterij Campylobacter jejuni na celice Caco-2. Sposobnost invazije v celice Caco-2 sta imela samo mutanta z mutacijo v genu rrpB in genu luxS. Ostali mutanti niso invadirali v celice Caco-2. Potrebne so nadaljnje raziskave za razvoj strategij, ki omejujejo število okužb in omogočajo nadzor bakterij v živilihType of material - master's thesis ; adult, seriousPublication and manufacture - Maribor : [T. Damijan], 2022Language - slovenianCOBISS.SI-ID - 136101891
Author
Damijan, Tjaša
Other authors
Langerholc, Tomaž, 1977- |
Šikić Pogačar, Maja |
Klančnik, Anja
Topics
Univerzitetna in visokošolska dela |
Campylobacter jejuni |
tvorba biofilma |
adhezija |
invazija |
celice Caco-2 |
Campylobacter jejuni |
biofilm formation |
adhesion |
invasion |
Caco-2 cells
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Damijan, Tjaša | ![]() |
Langerholc, Tomaž, 1977- | 23574 |
Šikić Pogačar, Maja | 29591 |
Klančnik, Anja | 22491 |
Source: Personal bibliographies
and: SICRIS
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