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Priprava s peroksidazo konjugiranih monoklonskih protiteles proti kokošjim imunoglobulinom razreda G (IgY) : diplomska naloga = Preparation of peroxidase conjugated monoclonal antibodies against G class of chicken immunoglobulins (IgY) : graduation thesisRejc, MatejaOkuÍbe perutnine v intenyivni reji so relativno pogoste in lahko povyrořajo veliko ekonomsko Ǵkodo. Veřino okuÍb lahko ugotovimo s seroloǴkimi testi, ki temeljijo na dokayovanju specifiřnih serumskih ... imunoglobulinov rayreda Z (IgZ), ki so ptiřji homolog sesalskemu rayredu G (IgG). Poleg ustreynega antigena so ya iyvedbo takǴnega testa potrebna protitelesa proti molekuli IgZ,ki so lahko poliklonskega ali monoklonskega iyvora. Iy jajřnega rumenjaka kokoǴi smo iyolirali IgZ, y njimi imuniyirali miǴ BALB/c in pripravili monoklonska protitelesa (mAb) proti kokoǴjim IgZ - mAb M2 -, ki so reagirala tudi y determinantami na IgZ fayana, purana in pava. Determinanta, ki jo prepoynavajo mAb M2, se nahaja v konstantni regiji teÍke verige kokoǴjih IgZ in ni glikoyilirana. Pridobljenim mAb M2 smo dolořili pH optimum, optimalno delovno koncentracijo v direktnem imunoencimskem testu, in ocenili afiniteto veyave. MAb M2 smo konjugirali s hrenovo peroksidayo in konjugat M2-p testirali v veř rayliřnih testih. M2-p je potencialno uporaben ya seroloǴko diagnostiko okuÍb kokoǴi, puranov, fayanov in pavov. Ker takǴnega konjugata natrÍiǴřu nismo yasledili, smo ya morebitno ponudbo iydelali specifikacijski list.Type of material - undergraduate thesis ; adult, seriousPublication and manufacture - Ljubljana : [M.Rejc] ; BF, Enota medoddelčnega študija mikrobiologije, 1999Language - slovenianCOBISS.SI-ID - 2287224
Author
Rejc, Mateja
Other authors
Habe, Franc |
Narat, Mojca
Topics
perutnina |
serološka diagnostika |
imuno-encimski testi |
imunoglobulini razreda Y |
monoklonska protitelesa |
konjugacija s peroksidazo |
konjugacija s peroksidazo |
poultry |
serological tests |
immunoenzyme assays |
chicken immunoglobulins of Y class |
monoclonal antibodies |
conjugated with peroxidase |
conjugated with peroxidase
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Študij mikrobiologije DN 0000000029 a IN: 0011658 Študij mikrobiologije DN 29 a IN: 0011658 |
available - outside loan, loan period: 1 months
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Študij mikrobiologije DN 0000000029 IN: 0011657 Študij mikrobiologije DN 29 IN: 0011657 |
available - outside loan, loan period: 1 months
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Habe, Franc | 02977 |
Narat, Mojca | 05008 |
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