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Comparison of culture-dependent and culture-independent methods for routine identification of airborne microorganisms in speleotherapeutic caves [Elektronski vir]Tomazin, Rok ...The effective identification of bacterial and fungal isolates is essential for microbiological monitoring in environments like speleotherapeutic caves. This study compares MALDI-TOF MS and the ... OmniLog ID System, two high-throughput culture-based identification methods. MALDI-TOF MS identified 80.0% of bacterial isolates to the species level, while the OmniLog ID System identified 92.9%. However, species-level matches between the methods were only 48.8%, revealing considerable discrepancies. For discrepant results, MALDI-TOF MS matched molecular identification at the genus level in 90.5% of cases, while the OmniLog ID System matched only in 28.6%, demonstrating MALDI-TOF MS’s superiority. The OmniLog ID System had difficulties identifying genera from the order Micrococcales. Fungal identification success with MALDI-TOF MS was 30.6% at the species level, potentially improvable with a customised spectral library, compared to the OmniLog ID System’s 16.7%. Metagenomic approaches detected around 100 times more microbial taxa than culture-based methods, highlighting human-associated microorganisms, especially Staphylococcus spp. In addition to Staphylococcus spp. and Micrococcus spp. as indicators of cave anthropisation, metagenomics revealed another indicator, Cutibacterium acnes. This study advocates a multi-method approach combining MALDI-TOF MS, the OmniLog ID System, culture-based, and metagenomic analyses for comprehensive microbial identification. Metagenomic sampling on nitrocellulose filters provided superior read quality and microbial representation over liquid sampling, making it preferable for cave air sample collection.Source: Microorganisms [Elektronski vir]. - ISSN 2076-2607 (Vol. 12, iss. 7, [article no.] 1427, 2024, str. 1-29)Type of material - e-article ; adult, seriousPublish date - 2024Language - englishCOBISS.SI-ID - 202944515
Author
Tomazin, Rok |
Cerar Kišek, Tjaša, 1980- |
Janko, Tea |
Triglav, Tina |
Strašek, Katja |
Špik, Vesna, 1976- |
Kukec, Andreja, 1982- |
Mulec, Janez, 1975- |
Matos, Tadeja
Topics
MALDI-TOF MS |
OmniLog ID System |
microbial identification |
metagenomics |
speleotherapy |
cave aerobiology |
nitrocellulose filters |
MALDI-TOF MS |
sistem OmniLog ID |
mikrobna identifikacija |
metagenomika |
speleoterapija |
jamska aerobiologija |
nitrocelulozni filtri
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Tomazin, Rok | 38488 |
Cerar Kišek, Tjaša, 1980- | 24350 |
Janko, Tea | ![]() |
Triglav, Tina | 54478 |
Strašek, Katja | 25989 |
Špik, Vesna, 1976- | 25990 |
Kukec, Andreja, 1982- | 30640 |
Mulec, Janez, 1975- | 20220 |
Matos, Tadeja | 18993 |
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26.7., 15.8. in 16.8. 2024 bo knjižnica ZAPRTA.