Faculty of Pharmacy, Lj. (FFALJ)
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Filogenetska uvrstitev ter določanje protimikrobne aktivnosti nekaterih gliv rodu Pestalotia de not = Taxonomic classification and determination of antibacterial activity of microfungi within genus Pestalotia de not : diplomsko deloIrman, ŠpelaPri iskanju novih zdravil se posluzujemo uporabe sodobnih tehnologij, sinteze novih derivatov ter iskanja virov morebitnih spojin vodnic. Endofitne glive predstavljajo se vedno sorazmerno neraziskano ... podroèje, ki obljublja stevilne nove farmakolosko aktivne spojine. Ker je bilo v predhodnem diplomskem delu ugotovljeno protimikrobno delovanje metanolno-vodnega izvleèka iz glive Truncatella hartigi Steyaert, smo se odloèili podrobneje prouèiti rod gliv Pestalotia de Not, v katerega Truncatella hartigi tudi uvrsèamo. Glive iskanega rodu smo najprej poiskali na naravnih gostiteljih, jih iz rastlinskega materiala izolirali ter pripravili èiste kulture na razliènih trdnih in tekoèem gojisèu za glive. Prouèevali smo njihovo rast pri razliènih pogojih. Iz nagojenih gliv smo pripravili vodno-metanolne in metanolne izvleèke. Pri preizkusanju protimikrobne aktivnosti pripravljenih izvleèkov nauporabljen sev bakterije Escherichia coli DH5Ž z difuzijsko, dilucijsko ter mikrodilucijsko metodo, nismo opazili bistvene protimikrobne aktivnosti le-teh. V okviru tega diplomskega dela smo se ukvarjali tudi s taksonomijo gliv rodu Pestalotia. Poleg morfoloskih znaèilnosti spor smo pri uvrsèanju v sistem uporabili tudi vrsto gostitelja ter v zadnjem èasu vedno bolj uporabljena nukleotidna zaporedja ITS1, 5.8S ter ITS2 regije, ki smo jih tudi sami doloèali. Iz nabranega rastlinskega materiala nam je uspelo izolirati vsaj tri vrste glivČ Pestalotiopsis funerea (Desmazieres) Steyaert oziroma Pestalotiopsis funereoides Steyaert, Pestalotiopsis uvicola (Spegazzini) Bissett ter Truncatella angustata (Persoon Č Link) Hughes.Type of material - undergraduate thesisPublication and manufacture - Ljubljana : [Irman Š.], 2003Language - slovenianCOBISS.SI-ID - 1320305
Author
Irman, Špela
Other authors
Štrukelj, Borut |
Jurca, Dušan |
Planinšek, Odon
Topics
Pestolatia de Not |
glive |
antibiotiki |
metode |
metode
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Knjižnica dp 0000000581 Irman, Špela Filogenetska IN: 0006972 Knjižnica dp 581 Irman, Špela Filogenetska IN: 0006972 |
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Irman, Špela | 24471 |
Štrukelj, Borut | 07849 |
Jurca, Dušan | ![]() |
Planinšek, Odon | 14935 |
Source: Personal bibliographies
and: SICRIS
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