Faculty of Pharmacy, Lj. (FFALJ)
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Primerjava različnih serološki testov za dokaz nevroborelioze = Comparison a different serological tests for evidence a neuroborreliose : diplomska nalogaRogina, MelitaLajmska borelioza je razsirjena povsod tam, kjer se nahajajo sèitasti klopi izrodu Ixodes. Povzroèa jo bakterija Borrelia burgdorferi sensu lato z epidemioloskim rezervoarjem v gozdnih zivalih. Klopi ... prenasajo borelije z ene zivalske vrste na drugo, tudi na èloveka, ki je v tem krogu le nakljuèni gostitelj. Najpomembnejsi klinièni znak, ki omogoèa diagnozo bolezni, je znaèilna kozna sprememba erythema migrans in se kaze kot obroèasta rdeèina z bledenjem v sredini. Bolezen se lahko na tej stopnji ozdravi spontano, vendar je mozno, da ostanejo v telesu zive spirohete, ki lahko pozneje povzroèijo nevroloske, srène in sklepne okvare.Prizadetost osrednjega in perifernega zivèevja pri lajmski boreliozi imenujemo nevroborelioza, ki jo v Evropi povzroèa predvsem B. garinii in redkeje B. afzelii, v ZDA pa samo B. burgdorferi sensu stricto. Zanesljivo diagnozo je v primeru, ko ni znaèilnih kliniènih znakov, tezko oz. nemogoèe postaviti. Takrat najpogosteje uporabimo specifiène seroloske teste, ker so ti najbolj enostavni. Ti testi soČ imunofluorescenèni test (IFT), encimsko imunski test (EIT), test imunoblot (western blot) in hemaglutinacijski test. Neposredno okuzbo dokazemo z veriznoreakcijo s polimerazo (PCR) in s kultivacijo.Namen diplomskega dela je bil, da izvedemo razliène seroloske teste za dokaz nevroborelioze.Serume in likvorje 67 bolnikov z nevroboreliozo smo preiskovali s 3 testiČ z indirektnimIFT, EIT in s testom imunoblot. Delujejo na principu reakcije antigenov in specifiènih protiteles. Kolièino protiteles najpogosteje izrazimos titrom ali vrednostjo OD. Pri indirektnem IFT smo za antigen vzeli sev B. afzelii, pri EIT preèisèene bièke B. burgdorferi sensu stricto in za test imunoblot smo uporabili specifiène rekombinantne borelijske antigene, ki so pripravljeni v E. coli. V serumu bolnikov je najveè pozitivnih na protitelesa IgM in IgG zaznal test imunoblot in sicer na protitelesa IgM pri 12ž38 (31,58 %), na protitelesa IgG pa pri 11ž38 (28,95 %). EIT je v serumu bolnikov zaznal 23ž67 (34,33 %) pozitivnih na protitelesa IgM in 44ž67 (65,67 %) pozitivnih na protitelesa IgG. Indirektni IFT pa je v serumu bolnikov zaznal 8ž67 (11,94 %) pozitivnih na protitelesa IgM in 34ž67 (50,75 %) na protitelesa IgG. Iz rezultatov, ki smo jih dobili, vidimo, da je najbolj obèutljiv imunoblot (western blot), nato sledi EIT in na zadnjem mestu je indirektni IFT, saj je zaznal najmanj pozitivnih, kar pomeni, da je najmanj obèutljiv.Type of material - undergraduate thesisPublication and manufacture - Ljubljana : [Rogina, M.], 2004Language - slovenianCOBISS.SI-ID - 1656689
Author
Rogina, Melita
Other authors
Marin, Jožica |
Ružić-Sabljić, Eva
Topics
bakterija B |
lajmska borelioza |
diagnostika |
testiranje
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Knjižnica dp 0000000616 Rogina Melita Primerjava IN: 0008048 Knjižnica dp 616 Rogina Melita Primerjava IN: 0008048 |
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Database name | Field | Year |
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Rogina, Melita | ![]() |
Marin, Jožica | 03172 |
Ružić-Sabljić, Eva | 13296 |
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