-
Določanje fenotipov alfa-1-antitripsina z izoelektričnim fokusiranjem = The determination of alpha-1-antitrypsin phenotypes by isoelectric focusingJoković, Živa ...Backgraund. The purpose of the work was to introduce the method in our clinical chemistry routine to enable fenotyping of alfa-a-antitrypsin (A1AT) in serum. Phenotypes determination is important for ... early fiagnose, proof and exclusion the other reasons in lung emphysema and liver disease. Methods. Isoelectrofocusing on polyacrylamide-gel (PAG) enables the resolution of the most common genetic variants of A1AT, which differ in only one amino acid or/and in side chain composition of carbohydrate moiety. Separated proteins were identified by immunofixation. Results. In patients' samples from Pediatric Gastroenterology Clinic in Ljubljana and in random laboratory samples in which A1AT determination was required (n=55), different phenotypes were found MZ (n=13), ZZ (n=6), MS (n=1), SZ (n=1), MM (n=20) and M in combination with rapidly migrating variants (n=4). In ten (n=10) samples recognition of phenotypes was uncertain. The method is not sensitive and accurate enough for typing variant M1, subclasses of M. Null and many others less frequent variant. Conclusions. There are no data about A1AT phenotype frequency in Slovenia. The ethod introduced is the beginning to approach it. We shell have to use also the methods of molecular biology in further work.Source: Zdravniški vestnik : glasilo Slovenskega zdravniškega društva = Slovenian medical journal = journal of Slovenian Medical Association. - ISSN 1318-0347 (Let. 65, št. 10, 1996, str. 549-551)Type of material - article, component partPublish date - 1996Language - slovenianCOBISS.SI-ID - 59097
Author
Joković, Živa |
Babič, Katja |
Logar-Car, Gordana |
Osredkar, Joško, 1955-
Topics
Alpha 1-antitrypsin |
Genetics |
Phenotype |
Isoelectric focusing |
Child |
Human
source: Zdravniški vestnik : glasilo Slovenskega zdravniškega društva = Slovenian medical journal = journal of Slovenian Medical Association. - ISSN 1318-0347 (Let. 65, št. 10, 1996, str. 549-551)
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|---|
Logar-Car, Gordana | 05322 |
Osredkar, Joško, 1955- | 10691 |
Select pickup location:
Material pickup by post
Notification
Subject headings in COBISS General List of Subject Headings
Select pickup location
Pickup location | Material status | Reservation |
---|
Please wait a moment.