-
1H NMR metabolomics of microbial metabolites in the four MW agricultural biogas plant reactors : a case study of inhibition mirroring the acute rumen acidosis symptomsMurovec, Boštjan ...In this study, nuclear magnetic resonance (1 H NMR) spectroscopic profiling was used to provide a more com- prehensive view of microbial metabolites associated with poor reactor performance in a ... full-scale 4 MW me-sophilic agricultural biogas plant under fully operational and also under inhibited conditions. Multivariate analyses were used to assess the significance of differences between reactors whereas artfiicial neural networks (ANN) were used to identify the key metabolites responsible for inhibition and their network of interaction. Based on the results of nm-MDS ordination the subsamples of each reactor were similar, but not identical, despite homogenization of the full-scale reactors before sampling. Hence, a certain extent of variability due to the size of the system under analysis was transferred into metabolome analysis. Multivariate analysis showed that fully active reactors were clustered separately from those containing inhibited reactor metabolites and were sig- ni fi cantly di ff erent. Furthermore, the three distinct inhibited states were signi fi cantly di ff erent from each other. The inhibited metabolomes were enriched in acetate, caprylate, trimethylamine, thymine, pyruvate, alanine, xanthine and succinate. The differences in the metabolic fingerprint between inactive and fully active reactors observed in this study resembled closely the metabolites differentiating the (sub) acute rumen acidosis inflicted and healthy rumen metabolomes, creating thus favorable conditions for the growth and activity of pathogenic bacteria. The consistency of our data with those reported before for rumen ecosystems shows that 1 H NMR based metabolomics is a reliable approach for the evaluation of metabolic events at full-scale biogas reactors.Source: Journal of environmental management. - ISSN 0301-4797 (Vol. 222, sept. 2018, str. 428-435)Type of material - article, component part ; adult, seriousPublish date - 2018Language - englishCOBISS.SI-ID - 4080008
Author
Murovec, Boštjan |
Makuc, Damjan, 1978- |
Kolbl Repinc, Sabina, 1982- |
Prevoršek, Zala |
Zavec, Domen, 1989- |
Šket, Robert, 1988- |
Pečnik, Klemen, 1986- |
Plavec, Janez |
Stres, Blaž
Topics
biokemija |
bioplin |
mikrobiologija |
metabolomika |
biogas plant |
1H NMR |
metabolomics |
chenomix |
reactor inhibition
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|---|
Murovec, Boštjan | 20183 |
Makuc, Damjan, 1978- | 24975 |
Kolbl Repinc, Sabina, 1982- | 34230 |
Prevoršek, Zala | 27547 |
Zavec, Domen, 1989- | ![]() |
Šket, Robert, 1988- | 37426 |
Pečnik, Klemen, 1986- | 39270 |
Plavec, Janez | 10082 |
Stres, Blaž | 19104 |
Select pickup location:
Material pickup by post
Notification
Subject headings in COBISS General List of Subject Headings
Select pickup location
Pickup location | Material status | Reservation |
---|
Please wait a moment.