Leitgeb, Maja ; Primožič, Mateja, 1975- ; Knez, Željko
Osredotočili smo se na študij aktivnosti in stabilnosti lipaz iz raznih virov ter proteinaze iz lateksa Carica papaje v vodnem mediju in plinih visoke gostote. Stabilnost in aktivnost lipaz iz: ...Pseudomonas flourescences, Rhizopus javanicus, Rhizopus niveus ter Porchine pancreas v vodnem mediju pri atmosferskem tlaku ter v superkritičnem CO▫$_2$▫ (SC CO▫$_2$▫) in v tekočem propanu pri tlaku 100 bar, 300 bar in temperaturi 40°C smo raziskovali na osnovi inkubiranja omenjenih lipaz v teh medijih in na osnovi uporabe lipaz kot katalizatorjev za esterifikacijo butanojske kisline z izo-amil alkoholom oz. etanolom. Rezultati so pokazali, da SC CO▫$_2$▫ deaktivira omenjene lipaze, medtem ko tekoči propan izboljša njihovo aktivnost v primerjavi z vodnim medijem za faktor 4 (za lipazo iz Porchine pancreasa) do 9 (za Rhizopus javanicus lipazo). Stabilnost proteinaze iz lateksa Carica papaje v SC CO▫$_2$▫ smo preučevali pri tlaku 300 bar in različnih temperaturah. Prav tako smo ugotavljali vpliv kompresije in ekspanzije CO▫$_2$▫ (300 bar, 50°C) na stabilnost proteinaze.
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