On silica gels immobilized cellulase as a biocatalyst for hydrolysis of carboxymethyl cellulose [Elektronski vir]
Primožič, Mateja, 1975- ...
Cellulase preparation Celluzyme 0.7T was immobilized on silica gels using the standard sol-gel procedure. To compare the properties and activity of immobilized enzymes, gels were dried by two ...different drying routes: conventionally in air at 40 °C (xerogels) and supercritically with CO2 at 40 °C and 100 bar (aerogels). The properties of enzyme preparations in the form of aerogel and xerogel were compared at atmospheric pressure and at the same time with the properties of crude enzyme preparation. The study of cellulase preparation (Celluzyme 0,7T) activity in three different forms in SC CO2 will be reported. The aerogel and xerogel cellulase preparations were sucessfully reused many times in a high-pressure batch system.
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