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Vpliv kemičnih retrakcijskih sredstev na celične kulture keratinocitov dlesne = The influence of chemical retraction agents on cell cultures of gingival keratinocytesKopač, Igor ; Sterle, Maksimiljan ; Batista, UrškaPrior to fixed prosthodontic impression procedures, temporary horizontal retraction of the free gingival tissue should be accomplished apically to the preparation finishing line. The ... mechanical-chemical method using cotton retraction cords of various sizes, impregnated with various retraction chemicals, is the most commonly employed retraction technique. Most retractionagents have low pH values and are therefore hazardous to the cut dentine and periodontal tissues. Sympathomimetic vasoconstrictors introduced recently have more physiological pH values and are free of systemic side-effects. The aims of the study were: first, to culture gingival keratinocytes, and second, to observe and compare the effects of two differentchemical retraction agents on the cultured cells. The most obvious differences were observed between control specimens and specimens treated with25 % aluminum chloride: the treated specimens had smoother cell surfaces and destroyed intercellular contacts, compared to the control specimens. The cultures treated with 0.05% tetrahydrozoline showed only minor surface changes, compared to the untreated cultures. Despite incomplete terminal differentiation in cultures, keratinocytes represent a suitable model for testing chemical retraction agents. The results contribute to the proper selection of retraction agents for clinical use.Source: Zobozdravstveni vestnik. - ISSN 0044-4928 (Letn. 55, št. 1, 2000, str. 3-11)Type of material - article, component partPublish date - 2000Language - slovenianCOBISS.SI-ID - 11369945
Author
Kopač, Igor |
Sterle, Maksimiljan |
Batista, Urška
Topics
gingiva |
Keratinocytes |
Drug effects |
Ultrastructure |
Rats, wistar |
Cells, cultured |
Microscopy, electron |
Aluminum |
Chlorides |
Imidazoles |
Culture media |
stomatologija |
dlesni |
obzobno tkivo |
protetika |
kemična sredstva
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Database name | Field | Year |
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Kopač, Igor | 08224 |
Sterle, Maksimiljan | 03400 |
Batista, Urška | 08588 |
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