Predicting the therapeutic result of repetitive transcranial magnetic stimulation (rTMS) treatment could save time and costs as ineffective treatment can be avoided. To this end, we presented a ...machine-learning-based strategy for classifying patients with major depression disorder (MDD) into responders (R) and nonresponders (NR) to rTMS treatment. Resting state EEG data were recorded using 32 electrodes from 88 MDD patients before treatment. Then, patients underwent 7 weeks of rTMS, and 46 of them responded to treatment. By applying Independent Component Analysis (ICA) on EEG, we identified the relevant brain sources as possible indicators of neural activity in the dorsolateral prefrontal cortex (DLPFC). This was served through estimating the generators of activity in the sensor domain. Subsequently, we added physiological information and placed certain terms and conditions to offer a far more realistic estimation than the classic EEG. Ultimately, those components mapped in accordance with the region of the DLPFC in the sensor domain were chosen. Features extracted from the relevant ICs time series included permutation entropy (PE), fractal dimension (FD), Lempel-Ziv Complexity (LZC), power spectral density, correlation dimension (CD), features based on bispectrum, frontal and prefrontal cordance, and a combination of them. The most relevant features were selected by a Genetic Algorithm (GA). For classifying two groups of R and NR, K-Nearest Neighbor (KNN), Support Vector Machine (SVM), and Multilayer Perceptron (MLP) were applied to predict rTMS treatment response. To evaluate the performance of classifiers, a 10-fold cross-validation method was employed. A statistical test was used to assess the capability of features in differentiating R and NR for further research. EEG characteristics that can predict rTMS treatment response were discovered. The strongest discriminative indicators were EEG beta power, the sum of bispectrum diagonal elements in delta and beta bands, and CD. The Combined feature vector classified R and NR with a high performance of 94.31% accuracy, 92.85% specificity, 95.65% sensitivity, and 92.85% precision using SVM. This result indicates that our proposed method with power and nonlinear and bispectral features from relevant ICs time-series can predict the treatment outcome of rTMS for MDD patients only by one session pretreatment EEG recording. The obtained results show that the proposed method outperforms previous methods.
Sugar beet is recalcitrant to in vitro tissue culture. Usually, proliferation of in vitro cultured rosette explants is a prerequisite for micropropagation. Although hormonal treatments can induce ...proliferation in sugar beet rosette explants, they may also result in some side effects. In vitro culture of sugar beet explants and some hormonal treatments make them more prone to hyperhydricity. Effects of media with different concentrations of 6-benzylaminopurine (BAP) and kinetin (Kin) on the proliferation and hyperhydricity of haploid sugar beet explants were investigated. It was observed that 0.2 mg L
Kin, with a reasonable amount of proliferation and minimum rate of hyperhydricity, performed better than BAP in different concentrations and combinations. The effect sizes of the treatments on the dependent variables were large. The correlation between proliferation and hyperhydricity of the treated explants was statistically negative and the association was large. However, the hormonal treatments without BAP or with the lowest amount of it produced the highest proliferation rate with the least hyperhydricity. The coefficient of determination was R
quadratic = 0.885. The results suggest that, in comparison with BAP, Kin is a potent plant growth regulator for the proliferation of sugar beet haploid explants that causes the least hyperhydricity. Although explants proliferated better in the presence of 0.01 mg L
BAP in combination with Kin than under Kin alone, the hyperhydricity of the proliferated explants decreased their suitability for in vitro propagation.
Plants show exceptional developmental plasticity and the ability to reprogram cell identities during regeneration. Although regeneration has been used in plant propagation for decades, we only ...recently gained detailed cellular and molecular insights into this process. Evidently, not all cell types have the same regeneration potential, and only a subset of regeneration-competent cells reach pluripotency. Pluripotent cells exhibit transcriptional similarity to root stem cells. In different plant regeneration systems, transcriptional reprogramming involves transient release of chromatin repression during pluripotency establishment and its restoration during organ or embryo differentiation. Incomplete resetting of the epigenome leads to somaclonal variation in regenerated plants. As single-cell technologies advance, we expect novel, exciting insights into epigenome dynamics during the establishment of pluripotency.
•Pluripotent cells establish RAM identity regardless of original tissue.•Decrease in chromatin repression promotes regeneration capacity.•Cell division dilutes DNA methylation; non-CG DNA methylation is restored slowly.•Reinforcement of CHH methylation occurs in callus and during somatic embryogenesis.•Epigenome instability and incomplete reprogramming underlie somaclonal variation.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
•Automated brain tumor diagnosis from brain MRI.•Specific preprocessing for improving the system accuracy.•Using a new modified CNN for the final diagnosis.•Using an improved design of political ...optimizer for the CNN arrangement optimization.
Manual detection of brain and tumor tissues takes a long time and is dependent on the state of the operator due to the great complexity of brain tissues. Experts are also required to study the images in order to discover these difficulties, rendering the traditional and outdated approaches ineffectual in their absence. As a result, using automated approaches for precise tumor examination will be quite beneficial. The use of magnetic resonance imaging technologies to diagnose brain cancers has garnered a lot of interest in recent years. One of the most generally utilized procedures in this field is magnetic resonance imaging, which has a great capability of revealing the interior structures of the human body. The present study uses an automated method to determine the tumorous cases from brain MRI. The images have been fed into an ideal convolutional neural network after being preprocessed. Here, a CNN optimized by a metaheuristic algorithm is used for providing a higher accuracy. The proposed CNN has been optimized by an improved version of political optimizer. The results are then compared with some other reported method to show its prominence toward the other methodologies.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
This study evaluated the effects of citicoline and silymarin nanomicelles (SMnm) in repeated restraint stress (RRS).
Mice were exposed to RRS for four consecutive days, 2 hrs. daily. On day 5 of the ...study, SMnm (25 and 50 mg/kg, i.p.) and citicoline (25 and 75 mg/kg), and a combination of them (25 mg/kg, i.p.) were initiated. On day 18, anxiety-like behavior, behavioral despair, and exploratory behavior were evaluated. The prefrontal cortex (PFC) and the hippocampus were dissected measuring brain-derived neurotrophic factor (BDNF), cAMP response element-binding protein (CREB), and tumor necrosis factor-alpha (TNF-α) through Western Blot and ELISA, respectively.
In RR-exposed mice, anxiety-like behavior in the elevated plus maze (EPM) was enhanced by reductions in open arm time (OAT%) P < 0.001, and open arm entry (OAE%) P < 0.001. In the forced swimming test (FST), the immobility increased P < 0.001 while the swimming and climbing reduced P < 0.001. In the open field test (OFT), general motor activity was raised P < 0.05. Further, body weights reduced P < 0.001, and tissue BDNF and pCREB expressions decreased P < 0.001 while TNF-α increased P < 0.001. Conversely, SMnm, citicoline and their combination could reduce anxiety-like behavior P < 0.001. The combination group reduced the depressive-like behaviors P < 0.001. Moreover, body weights were restored P < 0.001. Besides, BDNF and pCREB expressions increased while TNF-α reduced, P < 0.001.
The combination synergistically improved emotion-like behaviors, alleviating the inflammation and upregulating the hippocampal BDNF-mediated CREB signaling pathway.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Steviol glycosides (SGs) and gibberellic acids share a part of their biosynthesis pathways. Despite the widespread studies on the effect of gibberellic acid 3 (GA3), the effect of gibberellic acid 4 ...and 7 (GA4/7) on Stevia rebaudiana has never been studied. This study aimed at a comparative evaluation of different hormone effects, i.e., 1 mg L−1 GA4/7, 1 mg L−1 GA3, or 0.5 mg L−1 kinetin and 0.5 mg L−1 6-benzylaminopurine (BAP) (KB 0.5), on in vitro propagation, growth, morphological properties, and content of SGs in leaf samples of stevia. In comparison with the control group (hormone-free), the treatments of KB 0.5 or GA3 produced the highest biomasses and largest leaf areas. The three hormonal treatments produced a similar number of leaves, the ratio of fresh to dry weight, and leaf length. GA4/7-treated explants produced the highest ratio of leaf area to leaf length. The effect of GA4/7 on shoot elongation was greater than that of the control or even GA3. While the effect of GA3 on rebaudioside-A (Reb-A) production was similar to that of the control (16.2 and 18.04 mg g−1, respectively), GA4/7 resulted in a lower amount of it (13.31 mg g−1). Except for GA4/7, which induced more stevioside accumulation, the treatments’ effects were comparable to that of the control. The ratio of stevioside to Reb-A was the highest for GA4/7 (2.62), followed by GA3 (1.93), and then the two others. Sum of Reb-A and stevioside content was not changed by the use of any of the treatments.
•GA3 and GA4/7 effects on stevia growth were similar, except for auxiliary shoot length, which elongated with GA4/7.•The ratio of Stevioside to Reb-A content significantly increased in GA4/7 treated plants.•The effects of kinetin and BAP on each of the SG contents, ratio, and the total amount, were similar to the control.•GAs application in stevia plants has a negative effect on Reb-A accumulation, but not on Stevioside amount.•The results suggest that the GAs may change the ratio of Reb-A to Stevioside but not the total SG contents.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
The present paper describes a detailed study of a highly efficient protocol to multiply the number of haploids in sugar beet production and subsequent chromosome doubling. The protocol involves an ...experiment investigating factorial interactions between cold pretreatment, seven genotypes of sugar beet, and kinetin to improve haploid embryo induction. In addition, the effects of color of ovules and flower bud position on haploid embryo induction were investigated. After subjecting the data to analysis of variance or Student’s
t
test (
P
< .05), the effect sizes of the independent variables were also estimated. Cold pretreatment was effective in stimulating the ovules. The haploid embryo induction rate for 1-week cold pretreated ovules (9.01%) was higher than that of freshly cultured ones (6.15%). In comparison with hormone-free medium (5.16%), the gynogenesis rate for the media supplemented with 0.05 or 0.5 mg L
−l
kinetin increased to 7.58 and 10.05%, respectively. The genotype responses were significantly different. Interactions of kinetin × cold pretreatment, genotype × hormonal treatment, genotype × cold pretreatment, and the three-way interaction were statistically significant. Moreover, the main effects of flower bud position, ovule color, and comma-form ovule on gynogenic response were significant. After investigating the effect of 5 g L
−l
colchicine for 3, 5, or 7 min on one genotype’s (SG2) specimens, all the haploid plantlets from the other genotypes were treated for 5 min as the best treatment. The paper discusses interactions of the factors, which may be interesting for others aiming to breed doubled haploid sugar beet or possibly other related plant species.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OBVAL, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Plant establishment is highly correlated with successful germination and seedling growth, the most sensitive and critical stages. Abiotic stresses by their limiting and adverse effects on these ...stages finally result in poor yield. Therefore, investigating resistance to salinity among genotypes of a desired plant is highly recommended for anticipated drought seasons. To study the effects of salinity on seed germination and initial growth of seedlings, a factorial experiment was conducted based on a completely randomized design with four replicates using three wheat (Triticum aestivum) genotypes (Zagros, Gascogen and Kuhdasht). Salinity levels included 0 (control), 3.5, and 7 dS m-1 (deci-Siemens per meter). The results indicated that salinity had a significant influence on different germination indices (P <0.05). In all the genotypes, with an increase in salinity levels, indices including germination percentage, germination rate, seed vigor, coefficient of germination rate, coleoptile to plumule ratio, and daily germination mean decreased. Unlike the mentioned indices, the ratio of the radicle to plumule and the mean of germination time increased. Zagros genotype showed more tolerant than the other two. Based on these results, it seems that germination indices may be effectively used in selecting tolerant plants to salinity stress.
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CEKLJ, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
Salinity stress is one of the important agricultural problems in the world. A factorial experiment based on completely randomized design with four replications was conducted to evaluate the effects ...of phytohormones (gibberellic acid and abscisic acid) on the activity of antioxidant enzymes (peroxidase, superoxide dismutase and catalase), rubisco activity and content, and proline in three wheat cultivars (Gascogen, Zagros, and Kuhdasht) under control and salinity stress (3.5 and 7 dS m⁻¹). The results showed that salinity stress (3.5 and 7 dS m⁻¹) decreased the activity of catalase, rubisco, carboxylase, but increased peroxidase, superoxide dismutase activity and proline content. Gibberellic acid caused 58.03% increased in rubisco carboxylase activity in Zagros at 7 dS m⁻¹ in comparison with abscisic acid under salinity stress compared with the control plants in Kuhdasht. Activity of superoxide dismutase in Kuhdasht cultivar at 7 dS m⁻¹ salinity level showed 76.43% increased in Gascogen under salinity stress compared with the control plants with gibberellic acid application. The highest proline content as an osmolyte was found in Zagros at 7 dS m⁻¹ salinity level with abscisic acid (194 μmol g⁻¹ DM) application. Peroxidase activity increased 83.31% and catalase activity decreased 61.27% compared with the control plants in Zagros. Gibberellic acid application significantly prevented reduction in rubisco content under salinity stress. In conclusion, increased in peroxidase and superoxide dismutase activity and proline content decreased the adverse effects of salinity stress on studied cultivars. Also, the foliage spray of gibberellic acid enhanced and improved the growth condition. In this experiment, Zagros cultivar showed more tolerance to salinity stress than the other two cultivars.
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BFBNIB, GIS, IJS, KISLJ, NUK, PNG, UL, UM, UPUK
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