An experiment for the calculation of the degree of double bond conversion, after a polymerization reaction, of photopolymerizable liquid monomers using FTIR-ATR spectroscopy is reported. The ...experiment was successfully conducted with undergraduate students in materials engineering during the Fundamentals of Polymeric Material course. Students synthesized a Bis-GMA and TEGDMA copolymer through radical chain polymerization using visible light as the energy source (photopolymerization). Through the quantitative analysis of two absorption bands in the FTIR spectra of the monomer and the polymer, the νCC of the alkene group at 1638 cm–1 and the νCC of the aromatic ring at 1610 cm–1, students calculated the degree of double bond conversion after the polymerization reaction. Students acquired competencies in the synthesis and qualitative and quantitative characterization of polymeric materials through FTIR spectroscopy.
Full text
Available for:
IJS, KILJ, NUK, PNG, UL, UM
The relationship between pluralism and spirituality is explored in the context of a specific understanding of humanism, developed by the political philosopher William Connolly. He uses the term ...‘entangled humanism’ to express his ecological view on how humans relate to other forms of being, stressing the need for an attitude of partnership and care in the face of ecological crisis. To realize this non-anthropocentric form of humanism, we need our creativity and imagination, and a spiritual affinity characterized by openness and a commitment to shared goals. According to Connolly, we need a deep pluralism in which people are ready to defend their creed in public while realizing a productive ethos of political engagement with others of different convictions. We need to find one another in imaginative ideas and creative actions with regard to problems that confront us all and that are so urgent as to force us to collective action. According to Connolly, in working together, a connecting spiritual affinity can emerge. This view is elaborated upon with the help of John Dewey’s notions of moral imagination and religious trust, understood here as key elements of an entangled humanism. The article closes by describing a spirituality of the (im)possible that can be a source of inspiration for an entangled humanism that really makes a difference in our complex but rich world.
Mass spectrometry imaging (MSI) is a widely established technology; however, in the cardiovascular research field, its use is still emerging. The technique has the advantage of analyzing multiple ...molecules without prior knowledge while maintaining the relation with tissue morphology. Particularly, MALDI-based approaches have been applied to obtain in-depth knowledge of cardiac (dys)function. Here, we discuss the different aspects of the MSI protocols, from sample handling to instrumentation used in cardiovascular research, and critically evaluate these methods. The trend towards structural lipid analysis, identification, and “top-down” protein MSI shows the potential for implementation in (pre)clinical research and complementing the diagnostic tests. Moreover, new insights into disease progression are expected and thereby contribute to the understanding of underlying mechanisms related to cardiovascular diseases.
Full text
Available for:
DOBA, EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, IZUM, KILJ, KISLJ, MFDPS, NLZOH, NUK, OBVAL, OILJ, PILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UILJ, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
ABSTRACT We present Atacama Large Millimeter/submillimeter Array (ALMA) observations from the 2014 Long Baseline Campaign in dust continuum and spectral line emission from the HL Tau region. The ...continuum images at wavelengths of 2.9, 1.3, and 0.87 mm have unprecedented angular resolutions of 0 075 (10 AU) to 0 025 (3.5 AU), revealing an astonishing level of detail in the circumstellar disk surrounding the young solar analog HL Tau, with a pattern of bright and dark rings observed at all wavelengths. By fitting ellipses to the most distinct rings, we measure precise values for the disk inclination ( ) and position angle We obtain a high-fidelity image of the 1.0 mm spectral index ( ), which ranges from in the optically thick central peak and two brightest rings, increasing to 2.3-3.0 in the dark rings. The dark rings are not devoid of emission, and we estimate a grain emissivity index of 0.8 for the innermost dark ring and lower for subsequent dark rings, consistent with some degree of grain growth and evolution. Additional clues that the rings arise from planet formation include an increase in their central offsets with radius and the presence of numerous orbital resonances. At a resolution of 35 AU, we resolve the molecular component of the disk in HCO+ (1-0) which exhibits a pattern over LSR velocities from 2-12 km s−1 consistent with Keplerian motion around a ∼1.3 star, although complicated by absorption at low blueshifted velocities. We also serendipitously detect and resolve the nearby protostars XZ Tau (A/B) and LkH 358 at 2.9 mm.
Inhibition of target digestive enzymes is an accepted strategy to prevent diseases such as obesity and diabetes. Proanthocyanidins (PACs) are known for their ability to bind, inhibit, and precipitate ...enzymes, which makes them potential bioDrugs with an impact on the digestive process. PAC degree of polymerization (DP) is one of the structural features responsible for their differential inhibitory potency but the explanation for this phenomenon is still unclear. Pecan nut (Carya illinoinensis L.) kernels and nutshells are rich in oligomeric and polymeric PACs. We have used thiolysis and HPLC analyses to propose four theoretical model structures of PACs representative of four semipurified fractions obtained from pecan kernel and shell, which showed different inhibitory activity against intestinal lipases, amylases, and proteases. The noncovalent interactions between PACs and digestive enzymes were predicted by in silico methods through computational software. These observations are discussed in view of current literature on the biological effects of PACs with different DPs and allowed us to propose the hypothesis that “small oligomeric PACs could be digestive enzyme inhibitors due to their capacity to enter and bind the enzymes’ specific cavities better than polymers and oligomers of medium and high molecular weight.”
Full text
Available for:
BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
Mass spectrometry imaging (MSI) can analyze the spatial distribution of hundreds of different molecules directly from tissue sections usually placed on conductive glass slides to provide conductivity ...on the sample surface. Additional experiments are often required for molecular identification using consecutive sections on membrane slides compatible with laser capture microdissection (LMD). In this work, we demonstrate for the first time the use of a single conductive slide for both matrix-assisted laser desorption ionization (MALDI)-MSI and direct proteomics. In this workflow, regions of interest can be directly ablated with LMD while preserving protein integrity. These results offer an alternative for MSI-based multimodal spatial-omics.
Full text
Available for:
IJS, KILJ, NUK, PNG, UL, UM
The inhibitory activity and binding characteristics of caffeic acid,
-coumaric acid, quercetin and capsaicin, four phenolic compounds found in hot pepper, against porcine pancreatic lipase activity ...were studied and compared to hot pepper extract. Quercetin was the strongest inhibitor (IC
=(6.1±2.4) µM), followed by
-coumaric acid ((170.2±20.6) µM) and caffeic acid ((401.5±32.1) µM), while capsaicin and a hot pepper extract had very low inhibitory activity. All polyphenolic compounds showed a mixed-type inhibition. Fluorescence spectroscopy studies showed that polyphenolic compounds had the ability to quench the intrinsic fluorescence of pancreatic lipase by a static mechanism. The sequence of Stern-Volmer constant was quercetin, followed by caffeic and
-coumaric acids. Molecular docking studies showed that caffeic acid, quercetin and
-coumaric acid bound near the active site, while capsaicin bound far away from the active site. Hydrogen bonds and π-stacking hydrophobic interactions are the main pancreatic lipase-polyphenolic compound interactions observed.
The TgF344 rat model of Alzheimer's disease (AD) provides a comprehensive neuropathology presentation, with age-dependent development of tau tangles, amyloid-beta (AFormula: see text) plaques, ...neuronal loss, and increased gliosis. The behavioral trajectory of this model, particularly relating to spatial learning and memory, has yet to be fully characterized. The current experiment evaluated spatial working and reference memory performance, as well as several physiological markers of health, at 3 key age points in female TgF344-AD rats: 6-months, 9-months, and 12-months. At 6 months of age, indications of working and reference memory impairments were observed in transgenic (Tg) rats on the water radial-arm maze, a complex task that requires working and reference memory simultaneously; at 12 months old, Tg impairments were observed for two working memory measures on this task. Notably, no impairments were observed at the 9-month timepoint on this maze. For the Morris maze, a measure of spatial reference memory, Tg rats demonstrated significant impairment relative to wildtype (WT) controls at all 3 age-points. Frontal cortex, entorhinal cortex, and dorsal hippocampus were evaluated for AFormula: see text
expression via western blot in Tg rats only. Analyses of AFormula: see text
expression revealed age-dependent increases in all 3 regions critical to spatial learning and memory. Measures of physiological health, including heart, uterine, and body weights, revealed unique age-specific outcomes for female Tg rats, with the 9-month timepoint identified as critical for further research within the trajectory of AD-like behavior, physiology, and pathology.
Full text
Available for:
IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
Overexpression and secretion of the enzymes cathepsin D (CathD) and cathepsin L (CathL) is associated with metastasis in several human cancers. As a superfamily, extracellularly, these proteins may ...act within the tumor microenvironment to drive cancer progression, proliferation, invasion and metastasis. Therefore, it is important to discover novel therapeutic treatment strategies to target CathD and CathL and potentially impede metastasis. Graphene oxide (GO) could form the basis of such a strategy by acting as an adsorbent for pro-metastatic enzymes. Here, we have conducted research into the potential of targeted anti-metastatic therapy using GO to adsorb these pro-tumorigenic enzymes. Binding of CathD/L to GO revealed that CathD/L were adsorbed onto the surface of GO through its cationic and hydrophilic residues. This work could provide a roadmap for the rational integration of CathD/L-targeting agents into clinical settings.
Full text
Available for:
IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
The leafhopper Scaphoideus titanus is able to transmit 16SrV phytoplasmas agents of grapevine's flavescence dorée (FD) within 30–45 days, following an acquisition access period (AAP) of a few days ...feeding on infected plants as a nymph, a latency period (LP) of 3–5 weeks becoming meanwhile an adult, and an inoculation access period (IAP) of a few days on healthy plants. However, several aspects of FD epidemiology suggest how the whole transmission process may take less time, and may start directly with adults of the insect vector. Transmission experiments have been set up under lab condition. Phytoplasma‐free S. titanus adults were placed on broad bean (BB) plants (Vicia faba) infected by FD‐C (16SrV‐C) phytoplasmas for an AAP = 7 days. Afterwards, they were immediately moved onto healthy BB for IAP, which were changed every 7 days, obtaining three timings of inoculation: IAP 1, IAP 2 and IAP 3, lasting 7, 14 and 21 days from the end of AAP, respectively. DNA was extracted from plants and insects, and PCR tests were performed to identify FD phytoplasmas. Insects were dissected and fluorescence in situ hybridisation was made to detect the presence of phytoplasmas in midguts and salivary glands. The rate of infection in insects ranged 46–68% without significant differences among IAPs. Inoculation in plants succeeded in all IAPs, at a rate of 16–23% (no significant differences). Phytoplasma load was significantly higher in IAP 3 than IAP 1–2 for both plants and insects. Phytoplasmas were identified both in midgut and salivary glands of S. titanus at all IAP times. The possible implications of these results in the epidemiology of flavescence dorée are discussed.
The phytoplasmal agent of Flavescence dorée (FD) is transmitted to grapevine by the leafhopper Scaphoideus titanus. Even though 30‐45 days are reported to be required from acquisition (by nymphs) to inoculation (by adults), successful transmission could be achieved even after a shorter period, and it could start directly from adults. FD phytoplasma transmission experiments with S. titanus adults revealed that as soon as 14 days from acquisition the leafhoppers were able to transmit the pathogen to healthy broad beans. The results of this work open to new epidemiologic scenarios, which should be taken into account for FD management.
Full text
Available for:
BFBNIB, FZAB, GIS, IJS, IZUM, KILJ, NLZOH, NUK, OILJ, PILJ, PNG, SAZU, SBCE, SBMB, UL, UM, UPUK
PDF
