Adipose tissue derived stem cells (ADSCs) are mesenchymal stem cells identified within subcutaneous tissue at the base of the hair follicle (dermal papilla cells), in the dermal sheets (dermal sheet ...cells), in interfollicular dermis, and in the hypodermis tissue. These cells are expected to play a major role in regulating skin regeneration and aging-associated morphologic disgraces and structural deficits. ADSCs are known to proliferate and differentiate into skin cells to repair damaged or dead cells, but also act by an autocrine and paracrine pathway to activate cell regeneration and the healing process. During wound healing, ADSCs have a great ability in migration to be recruited rapidly into wounded sites added to their differentiation towards dermal fibroblasts (DF), endothelial cells, and keratinocytes. Additionally, ADSCs and DFs are the major sources of the extracellular matrix (ECM) proteins involved in maintaining skin structure and function. Their interactions with skin cells are involved in regulating skin homeostasis and during healing. The evidence suggests that their secretomes ensure: (i) The change in macrophages inflammatory phenotype implicated in the inflammatory phase, (ii) the formation of new blood vessels, thus promoting angiogenesis by increasing endothelial cell differentiation and cell migration, and (iii) the formation of granulation tissues, skin cells, and ECM production, whereby proliferation and remodeling phases occur. These characteristics would be beneficial to therapeutic strategies in wound healing and skin aging and have driven more insights in many clinical investigations. Additionally, it was recently presented as the tool key in the new free-cell therapy in regenerative medicine. Nevertheless, ADSCs fulfill the general accepted criteria for cell-based therapies, but still need further investigations into their efficiency, taking into consideration the host-environment and patient-associated factors.
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Focal Dermal Hypoplasia: Case Series Mansouri, Maria; Bouzid, Fatima Zohra; Amal, Said ...
Indian journal of dermatology,
2023 Jan-Feb, 2023-00-00, 20230101, 2023-01-01, Volume:
68, Issue:
1
Journal Article
Peer reviewed
Open access
Focal dermal hypoplasia (Goltz syndrome) is a genetic multisystem disorder characterized primarily by involvement of the skin associated to face, skeletal, and eyes anomalies. The objective of the ...present series is to shed light on this rare syndrome and these atypical manifestations.
Our study reports the case of five Moroccan patients who present typical clinical picture of the Goltz syndrome with some rare manifestations.
A total of 5 patients with Goltz syndrome were evaluated. All of them are female with one familial case. The age ranged from 8 months to 35 years. A characteristic Blaschkoid hypo- and hyper-pigmented skin lesions, congenital nodular fat herniation, and skin atrophy were present in all patients. Ocular manifestations were present in 80% of patients. Cranio-facial deformity was seen in 80% of patients. Short stature and intellectual delay were documented in 80% and 40% of patients, respectively. Limb abnormality was found in all patients. Two patients had a cleft lip, one of which unusual lateral facial cleft.
Genetic testing could not be performed in the present series.
Through this work we will discuss the different clinical signs and genetic aspects of Goltz syndrome and the interest of a good clinical expertise.
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Essential Oil (EO) extracted from Rosemary is known for its therapeutic, antifungal, stimulant and antibacterial effects. This study aimed to detect and quantify the adulteration of Rosemary ...essential oil with different percentages of eucalyptus essential oil, using two analytical techniques: gas chromatography with Flame Ionization Detection (GC-FID) and Fourier Transform Mid-infrared spectroscopy (FT-MIR), combined with chemometric tools such as Principal Component Analysis (PCA), Partial Least Squares regression (PLS-R) and support vector regression (SVR). The use of PCA on the results obtained from GC-FID and FT-MIR indicates the possibility of categorizing the data into two distinct groups: adulterated essential oil and non-adulterated essential oil. However, it is noted that GC-FID can only detect adulteration starting from 40%, while spectroscopy is capable of detecting lower percentages of adulteration. The use of PLS-R and SVR calibration models for adulteration quantification demonstrates high performance capabilities for both techniques (GC-FID and FT-MIR), as indicated by high R2 correlation coefficients indicating good fit, with lower root mean square error (RMSE) values demonstrating predictive accuracy. The results suggest that FT-MIR spectroscopy is preferable to GC-FID for the quantification and discrimination of adulterated essential oils. FT-MIR spectroscopy is considered superior to GC-FID due to its non-destructiveness, speed and lack of sample preparation.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Medicinal plants played an important role in traditional medicine for the treatment of diseases since antiquities. The aim of the study is to carry out an ethnobotanical survey on medicinal plants ...used traditionally in cancer treatment in the region of Greater Casablanca-Morocco, and to enhance the traditional herbal medicine knowledge. 110 traditional healers in the study area were interviewed face to face to response a survey questionnaire including the names of plants used in cancer treatment, used parts, methods of preparation, and route of administration. Indices of Fidelity Level (FL), Use Value (UV), and Relative Frequency of Citation (RFC) were calculated to identify the most effective plants used for cancer treatment. Eight species were specified including Aristolochia baetica, Aristolochia paucinervis, Bryonia dioica, Aquilaria malaccensis, Marrubium vulgare, Lavandula maroccana, Ephedra alata, and Euphorbia resinifera belonging to 7 families. Aristolochiaceae, Aquilariaceae, and Cucurbitaceae were the most useful families in cancer treatment with high significant indices of UV, FL, and RFC with values of 1, 100%, and 1, respectively. Roots and leaves were the most commonly used plant parts. Decoction and powder mixed with honey were the frequently used method for remedies preparation. The present study showed that the people living in Morocco’s economic capital are still highly dependent on traditional herbal medicine for the treatment of cancer. Therefore, it seems that herbal medicine still plays a crucial role in the primary healthcare system for the local population. During this survey, it was reported that even some plant families are highly toxic for humans like Aristolochiaceae that are frequently used in cancer treatment in the study area. As many people still rely on natural traditional medicine considering (it is safe with no side effects), so combined effort between all social categories including scientists and traditional healers should be established for involving the scientific validity of the used plants in the treatment of diseases.
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FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK, VSZLJ
Novel sensitive disposable sensors have been introduced for potentiometric determination of naproxen (NAP) based on single‐walled carbon nanotubes‐polyvinyl chloride (SWNTs‐PVC) composite ...incorporated of calix4arene as host‐guest molecular recognition element. The electrode matrix composition was optimized on the basis of the nature and content of the sensing element, cationic sites, plasticizer and nanomaterial. Remarkable selectivity and sensitivity towards NAP was achieved in the concentration range from 10−8 to 10−2 mol L−1 with anionic Nernstian compliance −61.0±0.0 mV decade−1. Modification with calixarene and carbon nanotubes improved the sensors performance through formation of NAP‐calixarene inclusion complex and promotion of the electron‐transfer processes which enhance the potential stability, response time, and shelf lifetime of the sensor. The proposed sensors were successfully applied for the potentiometric assay of NAP in dosage formulation and biological fluids under batch and flow injection analysis (FIA) conditions with average recoveries agreeable with the reported official methods.
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•FTIR and chemometry combination was developed for chemical analysis of gallstones.•PLS regression showed high correlation coefficients and lower prediction errors.•PLS calibration ...models were effective for quantifying cholesterol in gallstones.•UV–Vis technique confirmed the gallstones classification of the calibration model.•The developed spectroscopic method can offer reliable classification of gallstones.
Gallstones are stone-like deposits that most often develop inside the human gallbladder. In this study, the quantification of cholesterol gallstones was monitored using the Fourier transform mid-infrared (FT-MIR) spectroscopy technique and chemometrics tools. To detect the presence of outliers in the spectral data before developing a prediction model, principal component analysis (PCA) was utilized for qualitative analysis. The application of the partial least square (PLS) calibration models for the quantification of cholesterol demonstrated high-performance capabilities, as indicated by high values of correlation coefficients R2 greater than 0.999, and lower values of root mean square error (RMSE) less than 0.493. According to our results, FT-MIR spectroscopy combined with chemometrics approaches can be used successfully as a simple, quick, and non-destructive method for the quantification of cholesterol in biliary lithiasis.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
A 47-year-old, north african, male patient, has recently been diagnosed with pernicious anemia, treated with weekly intramuscular hydroxocobalamin. 6 weeks after its initiation, the patient presented ...a sudden, extensive and monomorphic eruption of inflammatory papulo-pustules and nodules, affecting the face, and the trunk. The eruption was pruritic, and comedones were also present, on the chest. The patient was diagnosed with vitamin B12-induced acneiform eruption. Levels of vitamin B12 were normalized. Hydroxocobalamin was therefore stopped and lymecycline was started, allowing a complete resolution of the lesions within 3 months. Drug intake, sudden and uncommon age of onset, pruritus, a monomorphic pattern and an involvement of extra-seborrheic areas are features that distinguish acneiform eruptions from acne vulgaris.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Background and Aim: Several strains of Aspergillus fumigatus produce mycotoxins that affect the health and productivity of dairy cattle, and their presence in dairy cattle feed is a serious concern. ...This study aimed to determine the densities of A. fumigatus and gliotoxin in commercial dairy feed.
Materials and Methods: More than 60 dairy feed samples were examined for fungal contamination, specifically for A. fumigatus, using phenotypic approaches and DNA sequencing of the internal transcribed spacer (ITS) and β-tubulin regions. Thin-layer chromatography and high-performance liquid chromatography (HPLC) were used to assess gliotoxin production in A. fumigatus. Real-time polymerase chain reaction (RT-PCR) was used to investigate the expression of gliZ, which was responsible for gliotoxin production. High-performance liquid chromatography was used to detect gliotoxin in feed samples.
Results: Aspergillus was the most commonly identified genus (68.3%). Aspergillus fumigatus was isolated from 18.3% of dairy feed samples. Only four of the 11 A. fumigatus isolates yielded detectable gliotoxins by HPLC. In total, 7/11 (43.7%) feed samples tested had gliotoxin contamination above the threshold known to induce immunosuppressive and apoptotic effects in vitro. The HPLC-based classification of isolates as high, moderate, or non-producers of gliotoxin was confirmed by RT-PCR, and the evaluation of gliZ expression levels corroborated this classification.
Conclusion: The identification of A. fumigatus from animal feed greatly depended on ITS and β-tubulin sequencing. Significant concentrations of gliotoxin were found in dairy cattle feed, and its presence may affect dairy cow productivity and health. Furthermore, workers face contamination risks when handling and storing animal feed.
Keywords: Aspergillus fumigatus, cattle feed, gliotoxin, gliZ, high-performance liquid chromatography, real-time polymerase chain reaction.
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•Nigella sativa and Syzygium aromaticum were tested as alternative litholytic agents against human gallstones.•GC-MS investigation of essential oils revealed eugenol and p-cymene as major ...compounds.•Both essential oils showed important dissolving capacity of cholesterol gallstone.•p-cymene has promising in vitro litholytic activity.•Molecular docking study revealed important affinity of eugenol with human 5-lipoxygenase (5-LOX).
Gallstones are a serious and widespread disease of the biliary system, which remain a costly disease and the major cause of hospital admissions for gastrointestinal problems. Natural products are a rich source of different volatile compounds such as terpenes that were found to be good dissolving solvents. Whereas, the potential of essential oils and their aromatic constituents has been rarely reported. Herein, we studied for the first time the litholytic activity of essential oils from Nigella sativa and Syzygium aromaticum, and their main compounds; p-cymene (49.25 %) and eugenol (49.92 %), respectively. The gallstone samples were classified as cholesterol and pigment types, using FTIR spectroscopy and UV-spectrophotometer analyses. All samples caused a deterioration in the morphological appearance of cholesterol gallstones with no effect on pigment gallstones. These volatile compounds, seem promising litholytic solvents as they reproduce better or close dissolving capacity to Methyl tertbutyl ether (MTBE) standard. Black cumin essential oil manifested with remarkable litholytic activity reached to a dissolution capacity of 90.07 ± 5.58 %, higher than that of clove with 59.52 ± 5.86 %, and that of MTBE standard (84.86 ± 2.93 %). Fascinating, p-cymene produced the most remarkable litholytic activity of 97.68 ± 2.11 %, leading to almost a total dissolution of the cholesterol gallstone sample, while that of eugenol was of 75.92 ± 6.02 %. The in-silico study suggested a strong lipoxygenase-inhibitory of eugenol compared with zileuton standard. The molecular docking experiments revealed that the important affinity of eugenol involving both hydrophobic and hydrogen bond interactions with human 5-lipoxygenase (5-LOX). Our study highlights for the first time the importance of aromatic structures from essential oils in gallstone dissolution. Eugenol and p-cymene contributed mainly to the activity of their essential oils, and could be effective litholytic agents able also to prevent gallstone formation through their potential anti-inflammatory intervention in the gallbladder.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
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