Abstract We collected specimens of Microcotyle spp. from two species of scorpaeniform fishes off Algeria, namely Scorpaena notata and Helicolenus dactylopterus . The identification of both fishes was ...confirmed by molecular barcoding of the COI gene. Sequences of COI gene were also obtained for both parasite species. The species from S. notata is described as Microcotyle algeriensis n. sp., on the basis of morphological differences from other species (number of clamps, number of spines in genital atrium, number of testes). Its COI sequence differs from M. sebastis Goto, 1894 (from Sebastes schlegeli from a fish farm in South Korea) by 14.6%. The species from H. dactylopterus is distinct from M. algeriensis on the basis of morphology (number of clamps, number of spines in genital atrium) and COI sequence (4.5% divergence) and is also distinct from M. sebastis in its COI sequence (12.3%). We refrained from describing it as new because M. sebastis , a species originally described from scorpaeniform fishes off Japan, has been recorded in various hosts in the North and South Pacific, Atlantic and Mediterranean (for the latter, in the same host, H. dactylopterus ). We believe that correct specific assignment of species of Microcotyle from scorpaeniform fishes needs a detailed morphological and molecular study of representatives from various locations and hosts.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
The family Plectanocotylidae includes parasites of the gills of marine fish; although nine genera and about 20 species have been described, almost no molecular information is available. Putting aside
...Diesing, 1850, supposedly a parasite of the white perch
, never found again since its original description, two species were valid within
Diesing, 1850 before this work:
(Van Beneden & Hesse, 1863) Llewellyn, 1941 and
Boudaya, Neifar & Euzet, 2006.
In this paper, we describe the third species of the genus
and perform a comparative morphological and molecular analysis of the three species and of
(Euzet & Suriano, 1974) Mamaev, 1976. Host fishes were also barcoded (COI) for confirmation of host identifications.
n. sp. is described from the gills of the streaked gurnard
collected off Algeria. The species is compared with specimens of
cf.
(from
) from the same locality and
and
(both from the longfin gurnard
). Molecules from
cf.
could not be compared with
from the type-host and type-locality and we kept the status of the Mediterranean specimens as pending. Algeria is a new geographic record for
and
.
n. sp. is distinguished from the other species found in the Mediterranean by the measurements of clamps, number of testes, and COI sequences, with notable divergence (7.8-11.8%) from the other two species of the genus.
We briefly present a list of currently known members of the family Plectanocotylidae, their biology and their hosts.
Background
Epithelial ovarian cancer (EOC) is the leading cause of death associated with gynecologic tumors. EOC is asymptomatic in early stages, so most patients are not diagnosed until late stages, ...highlighting the need to develop new diagnostic biomarkers. Mediators of the tumoral microenvironment may influence EOC progression and resistance to treatment.
Aim
To analyze immune checkpoints to evaluate them as theranostic biomarkers for EOC.
Patients and methods
Serum levels of 16 immune checkpoints were determined in EOC patients and healthy controls using the MILLIPLEX MAP® Human Immuno-Oncology Checkpoint Protein Magnetic Bead Panel.
Results
Seven receptors: BTLA, CD40, CD80/B7-1, GITRL, LAG-3, TIM-3, TLR-2 are differentially expressed between EOC and healthy controls. Serum levels of immune checkpoints in EOC patients are positively significantly correlated with levels of their ligands, with a higher significant correlation between CD80 and CTLA4 than between CD28 and CD80. Four receptors, CD40, HVEM, PD-1, and PD-L1, are positively associated with the development of resistance to Taxol-platinum-based chemotherapy. All of them have an acceptable area under the curve (>0.7).
Conclusion
This study has yielded a first panel of seven immune checkpoints (BTLA, CD40, CD80/B7-1, GITRL, LAG-3, TIM-3, TLR-2) associated with a higher risk of EOC and a second panel of four immune checkpoints (CD40, HVEM, PD-1, PD-L1) that may help physicians to identify EOC patients who are at high risk of developing resistance to EOC chemotherapy.
Specimens of Hexostoma thynni (Delaroche, 1811) Rafinesque, 1815 were collected from their type-host, the bluefin tuna Thunnus thynnus, caught off Algeria, i.e. close to the type-locality, off ...Mallorca, which is also in the Mediterranean. The species is briefly redescribed and compared to previous descriptions, under the same name or as its synonym Plagiopeltis duplicata Diesing, 1858, to ascertain identity of specimens. The three genera within the Hexostomatidae (Hexostoma Rafinesque, 1815, Neohexostoma Price, 1961 and Homostoma Unnithan, 1965) are briefly discussed, with comments on the fragility of characters used to distinguish them. Using next-generation sequencing, the complete mitogenome and the cluster of ribosomal genes (SSU, LSU, ITS1, ITS2, 5.8S) were obtained. The mitogenome is 14,649 bp long and codes for 12 protein-coding genes, 2 ribosomal RNA genes and 22 transfer RNA genes; its size is similar to other mitogenomes obtained from polyopisthocotylean monogeneans. A phylogeny based on concatenated mitogenome protein-coding genes from nine species of polyopisthocotylean monogeneans produced a tree in which the Hexostomatidae H. thynni was associated with other Mazocraeidea, such as Chauhaneidae and Diclidophoridae. This invalidates the hypothesis of Boeger & Kritsky (1993) of Hexostomatidae as sister-group to the Mazocraeidea and suggests the demise of the suborder Hexostomatinea Boeger & Kritsky, 1993. We insist on the usefulness of depositing parts of specimens used for molecular analyses, prepared on permanent slides, in a curated collection.
Gastrosplenic fistula is a rare and potentially fatal complication of various conditions. Lymphoma is the most common cause. It can occur spontaneously or after chemotherapy. Gastrosplenic fistula ...diagnosis can be confused with a splenic abscess because of the presence of air into the mass. The computed tomography identification of the fistulous tract is the key to a right diagnosis. Treatment modalities include surgical resection, chemotherapy, or a combination of both.
Here we report two patients with gastrosplenic fistula due to diffuse large B cell lymphoma. The first patient was a 54-year-old Caucasian woman with an enormous primary splenic diffuse large B cell lymphoma leading to the development of a spontaneous fistula in the stomach. The second patient was a 48-year-old Caucasian male patient with an enormous splenic diffuse large B cell lymphoma complicated by fistula after chemotherapy. Both patients died of septic shock several days after surgery.
Gastrosplenic fistula is a rare complication with a poor-prognosis, for which surgery is currently the preferred treatment.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
Introduction
Filgrastim is a granulocyte colony-stimulating factor (GSCF) used in some chemotherapy regimen to prevent febrile neutropenia. Most common reaction of filgrastim are aches and pain ...including headaches, nausea and skin rash.
Case report
We report the case of a patient who developed unusual, non-commonly reported adverse toxidermy to filgrastim. At first the eruption was limited to the lower members and genetics organs. Then it slowly spread across the whole body presenting as a polymorphic exanthematous-pustulosis lesions.
Management & Outcome
A cutaneous biopsy was done, identifying a toxidermy modified by systemic treatment. A pharmacological study linked the role of filgrastim to these lesions. After switching from filgrastim to lénograstim, his lesions are completely gone and haven't flared up again. Thus, clearly imputing the use of filgrastim.
Discussion
The cutaneous reaction that has reported with use of GSCF are sweet syndrome, erythema nodosum, pyoderma nodosum and pyoderma gangrenosum. As far as we know, no acute generalized exanthematous pustulosis due to GSCF has been reported.
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NUK, OILJ, SAZU, UKNU, UL, UM, UPUK
Approximately 5–10% of patients with Hodgkin lymphoma are refractory to initial treatment. The aim of our study was to assess the clinico-epidemiological profile, prognostic factors and treatment ...outcome. A retrospective study was conducted over a period of 12 years between June 2006 and January 2018 at the oncology department of Salah Azaïz Institute. Thirty-one patients were included. The median age was 27 years with a female predominance (sex ratio = 0.93).The majority had an advanced stage (61%). IGEV regimen was the most commonly used salvage chemotherapy (n = 14). Age above 30 years was predictive of treatment failure after salvage therapy (
p
= 0.003). IGEV regimen showed better results than ICE protocol in terms of response to salvage therapy (
p
= 0.048). Seven patients had salvage radiotherapy. Four patients had autologous stem cell transplant. Progressive disease (n = 12) was the main cause of non-eligibility of autologous stem cell tansplant. Overall survival and progression free survival at 3 years were 50% and 5% respectively. The prognostic factors influencing the overall survival were age above 30 years (
p
= 0.001), advanced Ann Arbor stage before progression (
p
= 0.02), advanced Ann Arbor stage of refractory Hodgkin lymphoma (
p
= 0.001), histological subtype (
p
= 0.001), CD20 expression (
p
= 0.027) and non-response to salvage therapy (
p
= 0.004). The prognostic factor influencing progression free survival was the non-response to salvage therapy (
p
= 0.045). The prognosis of refractory Hodgkin lymphoma remains poor. The current standard secondary treatment consists of combination therapy, usually followed by autologous stem cell transplantat. Innovative therapies are needed to improve the prognosis of refractory Hodgkin lymphoma.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Background:
Osteosarcoma is the most frequent bone cancer occurring in children and adolescents aged 10–20 years. Several prognostic factors have been identified by studies done at western centers. ...The aim of our study was to identify the prognostic factors in Tunisian patients in order to improve their management.
Methods:
We reviewed the data of localized limb osteosarcoma patients treated in Salah Azaïz Institute from January 1980 to December 2018. Patient’s treatment and survival variables were assessed. Patients received a neoadjuvant chemotherapy and underwent surgery in an expert center. They received afterward an adjuvant chemotherapy depending on the tumor necrosis assessed by Huvos.
Results:
Eighty-five patients were enrolled. Mean duration of follow-up was 30 months (range 1–297 months). Males were 1.6 times more frequent, median age was 17 (from 1 to 62 years). Conventional osteoblastic osteosarcoma was the most frequent histological subtype (77%). Median tumor size was 10 cm. Femoral location was the most frequent (60%). The overall average history of symptoms was 103 days (4 to 423 days). The 5-year overall-survival was 38% and the event free survival 32%. Tumor site, lactate dehydrogenase levels, high methotrexate levels at 24 h, clinical evaluation of the tumor perimeter, surgery type and delay of relapse were found to affect overall survival. Tumor site, Lactate dehydrogenase levels and clinical evaluation of the tumor perimeter affected the progression free survival.
Conclusion:
Demographic characteristics of Tunisian patients are mainly the same than worldwide. Femoral site, normal level of lactate dehydrogenase, a clinical response during neoadjuvant treatment, an R0 surgery, a delay of relapse over 2 years and Median H24 Methotrexate level superior to 4.4 µmol/l were associated with a better prognosis in our study.
Backgound
Bladder cancer (BCa) is a heterogeneous disease caused by the interaction between environmental and genetic risk factors. The goal of this case–control study was to evaluate the implication ...of a selected SNP panel in the risk of BCa development in a Tunisian cohort. We were also interested in studying the interaction between this predictive panel and environmental risk factors.
Methods
The case/control cohort was composed with 249 BCa cases and 255 controls. The designed Bladder cancer hereditary panel (BCHP) was composed of 139 selected variants. These variants were genotyped by an amplification-based targeted Next-Generation Sequencing (NGS) on the Ion Torrent Proton sequencer (Life Technologies, Ion Torrent technology).
Results
We have found that rs162555, rs2228000, rs10936599, rs710521, rs3752645, rs804276, rs4639, rs4881400 and rs288980 were significantly associated with decreased risk of bladder cancer. However the homozygous genotypes for
VPS37C
(rs7104333, A/A), MPG (rs1013358, C/C) genes or the heterozygous genotype for ARNT gene (rs1889740, rs2228099, rs2256355, rs2864873), GSTA4 (rs17614751) and APOBR/IL27 (rs17855750) were significantly associated with increased risk of bladder cancer development compared to reference group (OR 2.53, 2.34, 1.99, 2.00, 2.00, 1.47, 1.96 and 2.27 respectively). We have also found that non–smokers patients harboring heterozygous genotypes for ARNT/rs2864873 (A > G), ARNT/ rs1889740 (C > T) or GSTA4/rs17614751 (G–A) were respectively at 2.775, 3.069 and 6.608-fold increased risk of Bca development compared to non-smokers controls with wild genotypes. Moreover the
ARNT
CT (rs1889740),
ARNT
CG (rs2228099),
ARNT
TC (rs2864873) and
GSS
GA genotypes were associated with an increased risk of BCa even in absence of professional risk factors. Finally the decision-tree analysis produced a three major BCa classes. These three classes were essentially characterized by an intensity of tobacco use more than 20 pack years (PY) and the CYP1A2 (rs762551) genotype.
Conclusions
The determined association between environmental factors, genetic variations and the risk of Bca development may provide additional information to urologists that may help them for clinical assessment and treatment decisions. Nevertheless, the underlying mechanisms through which these genes or SNPs affect the clinical behavior of BCas require further studies.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
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