Plasma circulating tumour-specific microRNAs (miRNAs) are promising biomarkers of tumour presence and recurrence, especially for diseases whose best chance of successful treatment requires early ...diagnosis and timely surgery of an already malignant but not yet invasive tumour, such as colorectal cancer (CRC).
Expression levels of miRNAs previously found to be differently expressed in tumour vs normal colon tissues were investigated by quantitative real-time PCR in plasma from CRC patients and from healthy donors and confirmed in independent case control series. The validated miRNAs were also measured after surgery. Analyses were repeated on the subsets of haemolysis-free samples.
We identified four miRNAs differently expressed between the compared groups, two (miR-21 and miR-378) of which were validated. miR-378 expression decreased in non-relapsed patients 4-6 months after surgery and miR-378 ability to discriminate CRC patients from healthy individuals was not influenced by haemolysis levels of plasma samples.
The miRNA analysis on plasma samples represents a useful non-invasive tool to assess CRC presence as well as tumour-free status at follow-up. Plasma levels of miR-378 could be used to discriminate CRC patients from healthy individuals, irrespective of the level of haemoglobin of plasma samples.
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DOBA, EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, IZUM, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, SIK, UILJ, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
In men with castration-sensitive prostate cancer (CSPC), the HSD3B1 c.1245A>C variant has been reported to be associated with shorter responses to first-line androgen-deprivation therapy (ADT). Here, ...we evaluated the association between the inherited HSD3B1 c.1245A>C variant and outcomes from metastatic castration-resistant prostate cancer (mCRPC) after first-line treatment with abiraterone (Abi) or enzalutamide (Enza).
Patients with mCRPC (n = 266) were enrolled from two centers at the time of starting first-line Abi/Enza. Outcomes after Abi/Enza included best prostate-specific antigen (PSA) response, treatment duration, and overall survival (OS). Outcomes after first-line ADT were determined retrospectively, and included treatment duration and OS. As was prespecified, we compared patients with the homozygous variant HSD3B1 genotype (CC genotype) versus the combined group with the heterozygous (AC) and homozygous wild-type (AA) genotypes.
Among the 266 patients, 22 (8.3%) were homozygous for the HSD3B1 variant (CC). The CC genotype had no association with PSA response rate; the median Abi/Enza treatment duration was 7.1 months for the CC group and 10.3 months for the AA/AC group (log rank P = 0.34). Patients with the CC genotype had significantly worse OS, with median survival at 23.6 months for the CC group and 30.7 months for the AA/AC group (log rank P = 0.02). In multivariable analysis adjusting for age, Gleason score, PSA, prior chemotherapy, and M1 disease, the association between the CC genotype and OS remained significant (hazard ratio 1.78, 95% confidence interval 1.03–3.07, P = 0.04). Poor outcome after first-line ADT in the CC group was also observed when evaluating retrospective ADT duration data for the same combined cohort.
In this large two-center study evaluating the HSD3B1 c.1245 genotype and outcomes after first-line Abi/Enza, homozygous variant (CC) HSD3B1 genotype was associated with worse outcomes. Novel therapeutic strategies are needed to enable treatment selection based on this genetic marker.
•A two-center study evaluating treatment outcome in prostate cancer by genetic differences in the HSD3B1 gene.•Carriers of the HSD3B1 c.1245 CC genotype have poor prognosis treated with existing therapies targeting the androgen receptor.•Generally worse outcome after first-line androgen-deprivation therapy in men carrying the HSD3B1 c.1245 CC genotype.•Generally worse outcome in men carrying the HSD3B1 c.1245 CC genotype after treatment with abiraterone or enzalutamide.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Composite edible/degradable films produced with hydrocolloids and lipids can result in better functionality than films produced with the components, especially with respect to their barrier ...properties. Of the lipids, waxes produce the best water vapor barrier properties, but produce fragile/brittle films. The problem of incorporating lipids into a hydrocolloid in a homogenous way has still to be solved. The objective of this research was the incorporation of ‘Brazilian elemi’, a highly hydrophobic resinous exudate of the botanical family
Burseraceae, into gelatin films, using a blend of stearic and palmitic acids to dissolve the elemi, and subsequent emulsification of the filmogenic solution using triacetin as plasticizer. Films with the addition of acids, the blend and the blend and the elemi presented better water vapor barrier properties as compared to the gelatin/triacetin film. The mechanical resistance decreased with the addition of the lipids and the opacity and soluble matter increased. The confocal laser scanning microscopy showed the size distribution of the lipid drops and their localization in the matrix. They were not homogeneously incorporated despite the improvement in the water barrier property and the fact that the films appeared to be homogenous and malleable. The analyses by differential scanning calorimetry showed additional melting points besides that characteristic of the protein rich fraction, representing melting of the part of the lipid not incorporated into the filmogenic matrix. Dynamic mechanical calorimetry also showed the presence of more than one glass transition temperature (
T
g), indicating the occurrence of phase separation.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
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Follicular estradiol triggers luteolysis in cattle. Therefore, the control of follicle growth and steroidogenesis is expected to modulate luteal function and might be used as an ...anti‐luteolytic strategy to improve embryo survival. Objectives were to evaluate follicular dynamics, plasma concentrations of estradiol and luteal lifespan in Bos indicus and crossbred cows subjected to sequential follicular aspirations. From D13 to D25 of a synchronized cycle (ovulation = D1), Nelore or crossbred, non‐pregnant and non‐lactating cows were submitted to daily ultrasound‐guided aspiration of follicles >6 mm (n = 10) or to sham aspirations (n = 8). Diameter of the largest follicle on the day of luteolysis (7.4 ± 1.0 vs 9.7 ± 1.0 mm; mean ± SEM), number of days in which follicles >6 mm were present (2.3 ± 0.4 vs 4.6 ± 0.5 days) and daily mean diameter of the largest follicle between D15 and D19 (6.4 ± 0.2 vs 8.5 ± 0.3 mm) were smaller (p < 0.01) in the aspirated group compared with the control group, respectively. Aspiration tended to reduce (p < 0.10) plasma estradiol concentrations between D18 and D20 (2.95 ± 0.54 vs 4.30 ± 0.55 pg/ml). The luteal lifespan was similar (p > 0.10) between the groups (19.6 ± 0.4 days), whereas the oestrous cycle was longer (p < 0.01) in the aspirated group (31.4 ± 1.2 vs 21.2 ± 1.3 days). Hyperechogenic structures were present at the sites of aspiration and were associated with increase in concentration of progesterone between luteolysis and oestrus. It is concluded that follicular aspiration extended the oestrous cycle and decreased the average follicular diameter on the peri‐luteolysis period but failed to delay luteolysis.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SAZU, SBCE, SBMB, UL, UM, UPUK
There is conflicting evidence on the predictive role of
KRAS
status when cetuximab is added to oxaliplatin-based regimens. This study investigated the impact of
KRAS
,
NRAS
,
BRAF
,
PI3KCA
and
TP53
...status on outcome of elderly metastatic colorectal cancer patients enrolled in TEGAFOX-E (cetuximab, oxaliplatin and oral uracil/ftorafur—UFT) phase II study. Twenty-eight patients were enrolled and all were evaluable for safety and activity. Twenty-three specimens were analysed for
KRAS
,
BRAF
,
NRAS
,
PI3KCA
and
TP53
mutational status by means of polymerase chain reaction and correlated with objective response, progression-free survival and overall survival. An evident increase of response rate was noted in
KRAS
/
NRAS
wild-type cases (70 versus 33 %,
P
= 0.198).
KRAS
/
NRAS
wild-type status showed an independent association with a longer progression-free survival (44 versus 9 weeks,
P
= 0.009). Considering the combined assessment of
BRAF
,
KRAS
/
NRAS
and
TP53
, a trend towards an increase of response rate was noted in patients without mutations (83 versus 33 %,
P
= 0.063). Moreover, patients with all wild-type genes had significantly longer progression-free survival than patients with any mutation (48 versus 10 weeks,
P
= 0.007). As a single biomarker, only
KRAS
/
NRAS
proteins maintained an independent value for outcome prediction. Patients with
KRAS
/
NRAS
,
BRAF
and
TP53
wild-type tumours could derive the maximal benefits from treatment with cetuximab, oxaliplatin and UFT.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, VSZLJ, ZAGLJ
This short communication reports the impact of endometrial biopsies, uterine flushings and follicular fluid aspiration procedures at day 6 post artificial insemination (AI) on pregnancy rates. In ...Experiment 1, cows were timed AI (TAI) and assigned to the following treatment groups: control (n = 37), uterine flushing (n = 35) and endometrial biopsy (n = 38). On day 30 post AI, pregnancy rates were 40.5%, 33% and 28.5%, respectively (p > 0.1). Pregnancy rate on day 60 was lower (p < 0.004) in flushed cows than in the controls. In Experiment 2, oestrus was detected and cows were assigned to flushing (n = 32) or biopsy (n = 33) treatments 6 days after AI, which resulted in pregnancy rates of 31% and 36%, respectively (p > 0.1). In Experiment 3, cows were, 6 days after TAI, randomly assigned to the following treatments: control (n = 84) or aspiration of the largest follicle (n = 73). Pregnancy rates on day 30 post AI were 63.5% for the control group and 53% for the aspirated group (p > 0.1). In conclusion, uterine flushing and endometrial biopsy negatively affect pregnancy rates, but neither procedure can be considered to be incompatible with pregnancy maintenance. Follicular aspiration during pregnancy does not interact with pregnancy success. The amount and quality of samples obtained are compatible with the use of cellular and molecular analysis of uterine variables from cows that failed or succeeded on maintaining pregnancy.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SAZU, SBCE, SBMB, UL, UM, UPUK
The study area is located in eastern Ilgın, Konya – Turkey. The purpose of this study is to characterize the major and trace element contents in the coaly units in northern Ilgın (Konya – Turkey). ...Pliocene Dursunlu Formationis characterized by sandstone, siltstone, marl, mudstone and coal in the region. Lignite layers, where coals are found, have a varying thickness between 100 and 300 m. Dursunlu Formation coal samples have high contents of trace elements. Ti is the major element and Cs, Rb, Ta, Th, As, Nb, La, Ce, Pr, Nd, Sm are the trace elements found to be enriched with respect to world coal. Remainder elements are either lower or equal to the average values of elements in coaly units of Dursunlu Formation. Among the trace elements considered to pose a threat to the environment and human health, following values were determined: average As 133.2ppm, Th 12.1ppm.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Ethanol stimulates the production of prostaglandins in many species. The purpose of this study was to verify the effect of ethanol on the production of prostaglandin F2α (PGF2α) and luteolysis in ...bovine females. In the first experiment, Holstein cows at day 17 of the oestrous cycle were treated with 100% ethanol (0.05 ml/kg of body weight, IV; n = 5), saline (0.05 ml/kg of body weight, IV; n = 4) or synthetic prostaglandin (150 μg of D-cloprostenol/cow, IM; n = 4). The plasma concentrations of 13, 14-dihydro-15-keto PGF2α (PGFM; the main metabolite of PGF2α measured in the peripheral blood) were assessed by radioimmunoassay (RIA). There was an acute release of PGFM in response to ethanol comparing to other treatments (p less-than or equal to 0.05). However, only cows treated with PGF2α underwent luteolysis. In the second experiment, endometrial explants of cross-bred beef cows (n = 4) slaughtered at day 17 of the oestrous cycle were cultured for 4 h. During the last 3 h, the explants were cultured with medium supplemented with 0, 0.1, 1, 10 or 100 μl of 100% ethanol/ml. Medium samples were collected at hours 1 and 4 and concentrations of PGF2α were measured by RIA. Ethanol did not induce PGF2α production by the endometrium. In conclusion, ethanol does not cause luteolysis in cows because it stimulates production of PGF2α in extra-endometrial tissues.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SAZU, SBCE, SBMB, UL, UM, UPUK
Maden Bilgi Sistemleri hem metalik hem de metalik olmayan madenleri kapsamaktadır. Fosil yakıtlar içerisindeülkemizde en çok bulunan kömür için ayrı bir bilgi sisteminin oluşturulması, Türkiye’de ...madencilik açısındanönemli katkılar sağlayacak bir çalışma olacaktır. Kömür Maden Bilgi Sistemi (KMBS) oluşturulmasında önceliklepotansiyel kömür sahaları belirlenip bu sahalara rezerv bilgilerinin işlenmesi gerekmektedir. Sahaların işletilmedurumlarına göre tasnif edilmesinin ardından koordinat, detay rezerv, kurum, ruhsat gibi veri setleri işlenmelidir.Oluşturulacak sahalara jeolojik faktörler (birincil ve ikincil), çevresel faktörler, laboratuvar analiz sonuçları, insansağlığı ve meslek hastalıkları riskleri, işçi sağlığı ve güvenliği verileri gibi veri setleri işlenmesi ile oluşturulacaktematik haritalar, istenildiğinde farklı kullanıcılar tarafından erişim ve analiz edilebilme avantajı sağlamaktadır.Coğrafi Bilgi Sistemlerinde verilerin güncel tutulması sistemin etkin kullanımı açısından oldukça önemlidir.
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