Search results

Abstract Background: Angiogenesis is described as a complex process in which new microvessels sprout from endothelial cells of existing vasculature. This study aimed to determine whether long ... non-coding RNA (lncRNA) H19 induced the angiogenesis of gastric cancer (GC) and its possible mechanism. Methods: Gene expression level was determined by quantitative real-time polymerase chain reaction and western blotting. Cell counting kit-8, transwell, 5-Ethynyl-2′-deoxyuridine (EdU), colony formation assay, and human umbilical vein endothelial cells (HUVECs) angiogenesis assay as well as Matrigel plug assay were conducted to study the proliferation, migration, and angiogenesis of GC in vitro and in vivo . The binding protein of H19 was found by RNA pull-down and RNA Immunoprecipitation (RIP). High-throughput sequencing was performed and next Gene Ontology (GO) as well as Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis was conducted to analyze the genes that are under H19 regulation. Methylated RIP (me-RIP) assay was used to investigate the sites and abundance among target mRNA. The transcription factor acted as upstream of H19 was determined through chromatin immunoprecipitation (ChIP) and luciferase assay. Results: In this study, we found that hypoxia-induced factor (HIF)-1α could bind to the promoter region of H19, leading to H19 overexpression. High expression of H19 was correlated with angiogenesis in GC, and H19 knocking down could inhibit cell proliferation, migration and angiogenesis. Mechanistically, the oncogenic role of H19 was achieved by binding with the N 6 -methyladenosine (m 6 A) reader YTH domain-containing family protein 1 (YTHDF1), which could recognize the m 6 A site on the 3′-untransated regions (3′-UTR) of scavenger receptor class B member 1 (SCARB1) mRNA, resulting in over-translation of SCARB1 and thus promoting the proliferation, migration, and angiogenesis of GC cells. Conclusion: HIF-1α induced overexpression of H19 via binding with the promoter of H19, and H19 promoted GC cells proliferation, migration and angiogenesis through YTHDF1/SCARB1, which might be a beneficial target for antiangiogenic therapy for GC. more

Gastric cancer (GC) is one of the most common malignancies worldwide, but its molecular mechanisms remain unclear. Increasing evidence indicates that long non-coding RNAs (LncRNAs) play a pivotal ... role in various cancers recently. Our present study focused on exploring the function of long intergenic non-coding RNA 00473 (LINC00473) in GC. In this study, we found that LINC00473 expression was aberrantly increased in tumor tissues compared with the paired para-cancerous tissues. The expression of high LINC00473 in GC was notably correlated with a higher risk of lymphatic metastasis, a higher incidence of vascular cancer embolus, and advanced TNM stage. Further experiments showed that the overexpression of LINC00473 could promote the proliferation and metastasis of GC cells both in vitro and in vivo. The apoptosis of GC cells increased significantly by the decrease of LINC00473. Mechanistically, LINC00473 could sponge miR-16-5p in the cytoplasm and relieve its suppression of CCND2. Moreover, AQP3 was found to be a significant downstream target gene for LINC00473 through RNA transcriptome sequencing, as demonstrated by qRT-PCR and western blot. Overexpression of LINC00473 can partially reverse the effects of AQP3 decrease on GC proliferation and metastasis. LINC00473 regulated AQP3 expression through CREB was confirmed by western blot. Our research indicates that LINC00473/miR-16-5p/CCND2 axis plays a role in the proliferation of GC and modulates AQP3 to influence GC cell metastasis, making it a potential therapeutic target for GC. more

Abstract Pancreatic ductal adenocarcinoma (PDAC) is a lethal disease with limited treatment methods. Long non-coding RNAs (lncRNAs) have been found involved in tumorigenic and progression. The ... present study revealed that LINC01133, a fewly reported lncRNA, was one of 16 hub genes that could predict PDAC patients’ prognosis. LINC01133 was over-expressed in PDAC tumors compared to adjacent pancreas and could promote PDAC proliferation and metastasis in vitro and in vivo, as well as inhibit PDAC apoptosis. LINC01133 expression positively correlated to secreted phosphoprotein 1 (SPP1) expression, leading to an enhanced epithelial-mesenchymal transition (EMT) process. LINC01133 bound with actin-related protein 3 (Arp3), the complex reduced SPP1 mRNA degradation which increased SPP1 mRNA level, ultimately leading to PDAC proliferation. This research revealed a novel mechanism of PDAC development and provided a potential prognosis indicator that may benefit PDAC patients. more

Microradian tracking and pointing errors significantly affect the link performance and the bit error probability in free-space optical communication. This paper proposes the measurement method for ... point-ahead angle and coalignment error, which can significantly mitigate the tracking and pointing error, thus reduces the power of the free-space optical communication system. By using technologies of corner cube reflector, off-axis reflecting telescope, vacuum long-light path and anti-interference support structure design, microangles can be measured accurately, especially the coalignment error angle which is formed by two wide, incoherent beams nearly antiparallel to each other. This paper introduces the measuring equipment's structure, together with the measurement method and theoretical measurement model. The measuring accuracy analysis indicated that the measurement uncertainty of both point-ahead angle and coalignment error was superior to 0.2 μrad and the validation experimental results proved that measuring accuracies of point-ahead angle and coalignment error are all smaller than 1 μrad. more

Breast cancer is the most common cancer worldwide. A number of studies proposed that long non-coding RNA plays an essential role in the regulation of invasion and metastasis of various forms of ... malignancy, including lung cancer, gastric cancer, and bladder cancer. In this study, a long non-coding RNA(LncRNA) MAFG-AS1 was explored in detail to understand the significance in the etiology of breast cancer. The results indicated that expression of LncRNA MAFG-AS1 in the breast cancer tissues was significantly higher than the adjacent normal breast tissues and elevated expression level of LncRNA MAFG-AS1 was correlated to the larger tumor size, negative expression of ER, PR and lymph node metastasis. The potency of breast cancer proliferation, invasion, and metastasis was inhibited in the absence of LncRNA MAFG-AS1. Mechanically, LncRNA MAFG-AS1 was mainly located in the cytoplasm. The downstream target gene of LncRNA MAFG-AS1 was STC2 which might promote cell proliferation and metastasis in breast cancer and this study provides a new potential therapeutic target for breast cancer. more

Breast cancer is the leading cause of cancer-related death in women around the world. It is urgently needed to identify genes associated with tumorigenesis and prognosis, as well as to elucidate the ... molecular mechanisms underlying the oncogenic process. Long noncoding RNAs (lncRNAs) are widely involved in the pathological and physiological processes of organisms and play an important role as oncogenes or tumor suppressor genes, affecting the development and progression of tumors. In this study, we focused on terminal differentiation-induced non-coding RNA (TINCR) (GeneID:257000) and explore its role in the pathogenesis of breast cancer. The results showed that TINCR was increased in breast cancer tissue, and high expression level of TINCR was associated with older age, larger tumor size, and advanced TNM stage. High level of TINCR can promote proliferation and metastasis of breast cancer cells, while downregulation of TINCR induces G1-G0 arrest and apoptosis. Mechanismly, TINCR can bind to staufen1 (STAU1) and then guide STAU1 (GeneID:6780) to bind to OAS1 mRNA (NM_016816.4) to mediate its stability. Thus low level of OAS1(GeneID:4938) can lead to cell proliferation and migration. This result elucidates a new mechanism for TINCR in breast cancer development and provides a survival indicator and potential therapeutic target for breast cancer patients. more

Simulation technologies for optical transmission are used to simulate the course of laser long-distance transmission in space and reception by a free-space optical (FSO) communication terminal, which ... determines the receiving power and is one type of core technology for ground validation and detection. The simulated distance of the current optical transmission simulation methods is limited, and these methods fail to meet the continually increasing distance of FSO communication. In this paper, a method called “relay transmission” is proposed based on an optical fiber nanoprobe that can simulate an optical transmission at an arbitrary distance. Using this method, direct pointto- point performance tests using two optical terminals are realized in the laboratory, whose results are consistent with on-orbit results. The far-field pattern of the incident light is obtained by an off-axis multiband reflecting telescope system with a large aperture, and wave sampling is performed by an optical fiber nanoprobe whose powercollection aperture corresponds to that of the receiving antenna of the on-orbit terminal. The feasibility of the proposed method is demonstrated through theoretical analysis and a series of validation experiments. more

In this paper, an arbitrary distance optical transmission simulation method for free-space optical communication system is presented. Based on this method, direct point-to-point performance tests ... between two optical terminals can be realized in the laboratory, and the test results are equivalent to those on-orbit. A theoretical analysis of this method is presented in this paper. Verification experiments showed that there is a good linearity between the incoming power density and output photocurrent of the carbon nanotube (CNT); the relative power difference between the CNT and the charge-coupled device (CCD) camera is 4.75%, which can be ignored compared with the link redundancy. •Simulating any transmission distance for FSO communication by using a CNT.•Solving the problem that the transmission distance getting increasingly longer.•Including the theoretical model of the optical transmission method with any distance. more

亚油酸属ω-6型多不饱和脂肪酸，具有独特的生理功能，与人体健康密切相关。由于亚油酸大多来源于天然动植物油脂，其含量尚不能满足食品和制药业的需求，有效分离纯化亚油酸已成为近年来的研究热点。为给亚油酸的分离纯化研究提供参考，综述了近年来国内外亚油酸分离纯化技术的研究进展，系统介绍了尿素包合法、硝酸银硅胶柱色谱法、分子蒸馏法、脂肪酶酶解法、溶剂冷冻结晶法、高速逆流色谱法及耦合法的分离原理及研究成果，并分析了各分离纯化方法的优缺点。两种或多种分离纯化技术耦合可优势互补，提高分离效率，淀粉、三聚氰胺包合法和pH区带精制逆流色谱法则值得进一步深入研究。 Linoleic acid，which belongs to an omega-6 type polyunsaturated fatty acid, possesses unique physiological functions and is closely associated with human health. Linoleic acid mostly originates from natural animal fats and vegetable oils, and its content cannot meet the needs of the food and pharmaceutical industries. Therefore, developing effective technologies for the separation and purification of linoleic acid has become a research hotspot in recent years. In order to provide a reference for the separation and purification of linoleic acid, the separation and purification technologies of linoleic acid at home and abroad in recent years were reviewed, the separation principle and research results of the methods of urea adduction fractionation, silver nitrate impregnated-silica gel column chromatography, molecular distillation, lipase hydrolysis, solvent freezing crystallization, high-speed countercurrent chromatography and integration were systematically introduced, and both the pros and cons of each separation and purification method were analyzed. The integration of two or more separation and purification technologies can complement mutual advantage and improve separation efficiency. Moreover, starch, melamine complexation method and pH zone refined countercurrent chromatography are worthy of further research.

Angiogenesis is described as a complex process in which new microvessels sprout from endothelial cells of existing vasculature. This study aimed to determine whether long non-coding RNA (lncRNA) H19 ... induced the angiogenesis of gastric cancer (GC) and its possible mechanism. Gene expression level was determined by quantitative real-time polymerase chain reaction and western blotting. Cell counting kit-8, transwell, 5-Ethynyl-2'-deoxyuridine (EdU), colony formation assay, and human umbilical vein endothelial cells (HUVECs) angiogenesis assay as well as Matrigel plug assay were conducted to study the proliferation, migration, and angiogenesis of GC in vitro and in vivo . The binding protein of H19 was found by RNA pull-down and RNA Immunoprecipitation (RIP). High-throughput sequencing was performed and next Gene Ontology (GO) as well as Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis was conducted to analyze the genes that are under H19 regulation. Methylated RIP (me-RIP) assay was used to investigate the sites and abundance among target mRNA. The transcription factor acted as upstream of H19 was determined through chromatin immunoprecipitation (ChIP) and luciferase assay. In this study, we found that hypoxia-induced factor (HIF)-1α could bind to the promoter region of H19, leading to H19 overexpression. High expression of H19 was correlated with angiogenesis in GC, and H19 knocking down could inhibit cell proliferation, migration and angiogenesis. Mechanistically, the oncogenic role of H19 was achieved by binding with the N 6 -methyladenosine (m 6 A) reader YTH domain-containing family protein 1 (YTHDF1), which could recognize the m 6 A site on the 3'-untransated regions (3'-UTR) of scavenger receptor class B member 1 (SCARB1) mRNA, resulting in over-translation of SCARB1 and thus promoting the proliferation, migration, and angiogenesis of GC cells. HIF-1α induced overexpression of H19 via binding with the promoter of H19, and H19 promoted GC cells proliferation, migration and angiogenesis through YTHDF1/SCARB1, which might be a beneficial target for antiangiogenic therapy for GC. more