Clathrin-mediated endocytosis (CME) and its core endocytic machinery are evolutionarily conserved across all eukaryotes. In mammals, the heterotetrameric adaptor protein complex-2 (AP-2) sorts plasma ...membrane (PM) cargoes into vesicles via the recognition of motifs based on Tyr or di-Leu in their cytoplasmic tails. However, in plants, very little is known about how PM proteins are sorted for CME and whether similar motifs are required. In Arabidopsis (
), the brassinosteroid (BR) receptor BR INSENSITIVE1 (BRI1) undergoes endocytosis, which depends on clathrin and AP-2. Here, we demonstrate that BRI1 binds directly to the medium AP-2 subunit (AP2M). The cytoplasmic domain of BRI1 contains five putative canonical surface-exposed Tyr-based endocytic motifs. The Tyr-to-Phe substitution in Y
KAI reduced BRI1 internalization without affecting its kinase activity. Consistently, plants carrying the BRI1
mutation were hypersensitive to BRs. Our study demonstrates that AP-2-dependent internalization of PM proteins via the recognition of functional Tyr motifs also operates in plants.
Asparagopsis taxiformis (AT) is a source of multiple halogenated compounds and, in a limited number of studies, has been shown to decrease enteric CH4 emission in vitro and in vivo. Similarly, ...oregano has been suggested as a potential CH4 mitigating agent. This study consisted of 2 in vitro and 2 in vivo experiments. Experiment (Exp.) 1 was aimed at establishing the effect of AT on CH4 emission in vitro. Two experiments (Exp. 2 and 3) with lactating dairy cows were conducted to determine the antimethanogenic effect of AT and oregano (Exp. 3) in vivo. Another experiment (Exp. 4) was designed to investigate stability of bromoform (CHBr3) in AT over time. In Exp. 3, 20 Holstein cows were used in a replicated 4 × 4 Latin square design with four 28-d periods. Treatments were basal diet (control) or basal diet supplemented with (dry matter basis) 0.25% AT (LowAT), 0.50% AT (HighAT), or 1.77% oregano (Origanum vulgare L.) leaves. Enteric gas emissions were measured using the GreenFeed system (C-Lock Inc., Rapid City, SD), and rumen samples were collected for fermentation analysis using the ororuminal technique. In Exp.1 (in vitro), relative to the control, AT (at 1% dry matter basis, inclusion rate) decreased CH4 yield by 98%. In Exp. 3, HighAT decreased average daily CH4 emission and CH4 yield by 65% and 55%, respectively, in experimental periods 1 and 2, but had no effect in periods 3 and 4. The differential response to AT among experimental periods was likely a result of a decrease in CHBr3 concentration in AT over time, as observed in Exp. 4 (up to 84% decrease in 4 mo of storage). In Exp. 3, H2 emission was increased by AT and, as expected, the proportion of acetate in the total volatile fatty acids in the rumen was decreased and those of propionate and butyrate were increased by HighAT compared with the control. Compared with the control, HighAT decreased dry matter intake, milk yield, and energy-corrected milk yield in Exp. 3. Milk composition was not affected by treatment, except lactose percentage and yield were decreased by HighAT. Concentrations of iodine and bromide in milk were increased by HighAT compared with the control. Milk CHBr3 concentration and its organoleptic characteristics were not different between control and HighAT. Oregano had no effect on CH4 emission or lactational performance of the cows in Exp. 3. Overall, AT included at 0.50% in the ration of dairy cows can have a large mitigation effect on enteric CH4 emission, but dry matter intake and milk production may also decrease. There was a marked decrease in the CH4 mitigation potential of AT in the second half of Exp. 3, likely resulting from CHBr3 decay over time.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
ABSTRACT We present a measurement of the B-mode polarization power spectrum (the BB spectrum) from 100 of sky observed with SPTpol, a polarization-sensitive receiver currently installed on the South ...Pole Telescope. The observations used in this work were taken during 2012 and early 2013 and include data in spectral bands centered at 95 and 150 GHz. We report the BB spectrum in five bins in multipole space, spanning the range , and for three spectral combinations: 95 GHz × 95 GHz, 95 GHz × 150 GHz, and 150 GHz × 150 GHz. We subtract small (<0.5 in units of statistical uncertainty) biases from these spectra and account for the uncertainty in those biases. The resulting power spectra are inconsistent with zero power but consistent with predictions for the BB spectrum arising from the gravitational lensing of E-mode polarization. If we assume no other source of BB power besides lensed B modes, we determine a preference for lensed B modes of 4.9 . After marginalizing over tensor power and foregrounds, namely, polarized emission from galactic dust and extragalactic sources, this significance is 4.3 . Fitting for a single parameter, , that multiplies the predicted lensed B-mode spectrum, and marginalizing over tensor power and foregrounds, we find , indicating that our measured spectra are consistent with the signal expected from gravitational lensing. The data presented here provide the best measurement to date of the B-mode power spectrum on these angular scales.
ABSTRACT We present a measurement of the cosmic microwave background (CMB) gravitational lensing potential using data from the first two seasons of observations with SPTpol, the ...polarization-sensitive receiver currently installed on the South Pole Telescope. The observations used in this work cover 100 deg2 of sky with arcminute resolution at 150 GHz. Using a quadratic estimator, we make maps of the CMB lensing potential from combinations of CMB temperature and polarization maps. We combine these lensing potential maps to form a minimum-variance (MV) map. The lensing potential is measured with a signal-to-noise ratio of greater than one for angular multipoles between . This is the highest signal-to-noise mass map made from the CMB to date and will be powerful in cross-correlation with other tracers of large-scale structure. We calculate the power spectrum of the lensing potential for each estimator, and we report the value of the MV power spectrum between as our primary result. We constrain the ratio of the spectrum to a fiducial ΛCDM model to be AMV = 0.92 0.14 (Stat.) 0.08 (Sys.). Restricting ourselves to polarized data only, we find APOL = 0.92 0.24 (Stat.) 0.11 (Sys.). This measurement rejects the hypothesis of no lensing at using polarization data alone, and at using both temperature and polarization data.
We present a constraint on the tensor-to-scalar ratio, r, derived from measurements of cosmic microwave background (CMB) polarization B-modes with "delensing," whereby the uncertainty on r ...contributed by the sample variance of the gravitational lensing B-modes is reduced by cross-correlating against a lensing B-mode template. This template is constructed by combining an estimate of the polarized CMB with a tracer of the projected large-scale structure. The large-scale-structure tracer used is a map of the cosmic infrared background derived from Planck satellite data, while the polarized CMB map comes from a combination of South Pole Telescope, bicep/Keck, and Planck data. We expand the bicep/Keck likelihood analysis framework to accept a lensing template and apply it to the bicep/Keck dataset collected through 2014 using the same parametric foreground modeling as in the previous analysis. From simulations, we find that the uncertainty on r is reduced by ∼10%, from σ(r)=0.024 to 0.022, which can be compared with a ∼26% reduction obtained when using a perfect lensing template or if there were zero lensing B-modes. Applying the technique to the real data, the constraint on r is improved from r0.05<0.090 to r0.05<0.082 (95% C.L.). This is the first demonstration of improvement in an r constraint through delensing.
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CMK, CTK, FMFMET, IJS, NUK, PNG, UM
ABSTRACT
The use of galaxy clusters as precision cosmological probes relies on an accurate determination of their masses. However, inferring the relationship between cluster mass and observables from ...direct observations is difficult and prone to sample selection biases. In this work, we use weak lensing as the best possible proxy for cluster mass to calibrate the Sunyaev–Zel’dovich (SZ) effect measurements from the APEX-SZ experiment. For a well-defined (ROSAT) X-ray complete cluster sample, we calibrate the integrated Comptonization parameter, YSZ, to the weak-lensing derived total cluster mass, M500. We employ a novel Bayesian approach to account for the selection effects by jointly fitting both the SZ Comptonization, YSZ–M500, and the X-ray luminosity, Lx–M500, scaling relations. We also account for a possible correlation between the intrinsic (lognormal) scatter of Lx and YSZ at fixed mass. We find the corresponding correlation coefficient to be $r= 0.47_{-0.35}^{+0.24}$, and at the current precision level our constraints on the scaling relations are consistent with previous works. For our APEX-SZ sample, we find that ignoring the covariance between the SZ and X-ray observables biases the normalization of the YSZ–M500 scaling high by 1–2σ and the slope low by ∼1σ, even when the SZ effect plays no role in the sample selection. We conclude that for higher precision data and larger cluster samples, as anticipated from on-going and near-future cluster cosmology experiments, similar biases (due to intrinsic covariances of cluster observables) in the scaling relations will dominate the cosmological error budget if not accounted for correctly.
ABSTRACT We present measurements of E-mode polarization and temperature-E-mode correlation in the cosmic microwave background using data from the first season of observations with SPTpol, the ...polarization-sensitive receiver currently installed on the South Pole Telescope (SPT). The observations used in this work cover 100 of sky with arcminute resolution at 150 GHz. We report the E-mode angular auto-power spectrum (EE) and the temperature-E-mode angular cross-power spectrum (TE) over the multipole range 500 < ≤ 5000. These power spectra improve on previous measurements in the high- (small-scale) regime. We fit the combination of the SPTpol power spectra, data from Planck, and previous SPT measurements with a six-parameter ΛCDM cosmological model. We find that the best-fit parameters are consistent with previous results. The improvement in high- sensitivity over previous measurements leads to a significant improvement in the limit on polarized point-source power: after masking sources brighter than 50 mJy in unpolarized flux at 150 GHz, we find a 95% confidence upper limit on unclustered point-source power in the EE spectrum of at , indicating that future EE measurements will not be limited by power from unclustered point sources in the multipole range , and possibly much higher in
ABSTRACT The millimeter transient sky is largely unexplored, with measurements limited to follow-up of objects detected at other wavelengths. High-angular-resolution telescopes, designed for ...measurement of the cosmic microwave background (CMB), offer the possibility to discover new, unknown transient sources in this band-particularly the afterglows of unobserved gamma-ray bursts (GRBs). Here, we use the 10 m millimeter-wave South Pole Telescope, designed for the primary purpose of observing the CMB at arcminute and larger angular scales, to conduct a search for such objects. During the 2012-2013 season, the telescope was used to continuously observe a 100 deg2 patch of sky centered at R.A. 23h30m and decl. −55° using the polarization-sensitive SPTpol camera in two bands centered at 95 and 150 GHz. These 6000 hr of observations provided continuous monitoring for day- to month-scale millimeter-wave transient sources at the 10 mJy level. One candidate object was observed with properties broadly consistent with a GRB afterglow, but at a statistical significance too low (p = 0.01) to confirm detection.
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