Most lung cancer patients die of metastasis. Recent studies have indicated that dysregulated microRNAs (miRNAs) are involved not only in tumorigenesis, but also in metastasis. In the present study, ...we found that over-expression of miR-26a-5p potentiated the migration and invasion of lung cancer cells evidenced by wound healing assay and transwell assay, and metastasis-related genes MMP-9 and CD44 were up-regulated. We identified integrin-beta8 (ITGβ8) as a novel target of miR-26a, and ITGβ8 expression was negatively correlated with miR-26a-5p expression in lung cancer specimens. Mechanism study showed that miR-26a-5p enhanced lung cancer cell metastasis via activation of Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) pathway, and ITGβ8 mediated the activation of JAK2/STAT3 pathway by miR-26a-5p. By using in vivo imaging technology, we found that miR-26a-5p enhanced both tumor growth and metastasis in vivo; and activated JAK2/STAT3 pathway. Taken together, our results demonstrated that miR-26a-5p potentiated lung cancer cell metastasis via JAK2/STAT3 pathway by targeting ITGβ8. This finding provides insights into the mechanism underlying miRNAs regulation on lung cancer metastasis; and suggests miR-26a-5p as a therapeutic target for lung cancer treatment.
•90% of lung cancer patients die of metastasis.•miR-26a-5p enhances metastasis potential of lung cancer both in vitro and in vivo.•ITGβ8 is identified as a novel target of miR-26a-5p.•miR-26a-5p enhances lung cancer cell metastasis via activation of JAK2/STAT3 pathway.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
Post translational modifications (PTMs) can change the properties of a protein by covalent addition of functional groups to one or more amino acids, and influence almost all aspects of normal cell ...biology and pathogenesis. Lactylation is a novel identified PTM, and has been found in both histone and non-histone proteins. Since associated with the end product of glycolysis-- lactate, lactylation modification could provide a new perspective for understanding the relationship between metabolic reprogramming and epigenetic modifications. Accumulated evidences suggest that lactylation play important roles in tumor progression and links to poor prognosis in clinical studies. Histone lactylation can affect gene expression in tumor cells and immunological cells, further promoting tumor progression and immune suppression. Lactylation on non-histone proteins can also regulate tumor progression and drug resistance. In this review, we aimed to summarize the roles of lactylation in cancer progression, microenvironment int
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FFLJ, NUK, ODKLJ, UL, UM, UPUK, VSZLJ
•Ethanol yields of SFA1OE were around 0.492 g/g totalsugars in different hydrolysates.•Diploid strain SQ-2 displays improved ethanol yield and high temperature resistance.•Contributions of gene SFA1 ...on ethanol yields were evaluated in various hydrolysates.•SFA1OE with high ethanol yield fits to alkaline-distilled sweet sorghum bagasse.
Here, an engineered Saccharomyces cerevisiae strain SFA1OE was constructed by overexpressing SFA1 in a reported WXY70 with effective six-gene clusters. Under simulated maize hydrolysate, SFA1OE produced an ethanol yield of 0.492 g/g totalsugars within 48 h. The productivity of SFA1OE was comprehensively evaluated in typical hydrolysates from stalks of maize, sweet sorghum, wheat and Miscanthus. Within 48 h, SFA1OE achieved an ethanol yield of 0.489 g/g totalsugars in the optimized hydrolysate of alkaline-distilled sweet sorghum bagasse derived from Advanced Solid-State Fermentation process. By crossing SFA1OE with a DQ1-derived haploid strain, we obtained an evolved diploid strain SQ-2, exhibiting improved ethanol production and thermotolerance. This study demonstrates that overexpressing SFA1 enables efficient fermentation performance in different lignocellulosic hydrolysates, especially in the hydrolysate of alkaline-distilled sweet sorghum bagasse. The increased cellulosic bioethanol production of SFA1OE provides a promising platform for efficient biorefineries.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
L. is one of the large genera in the Apiaceae family. In a previous study, the molecular phylogenies of
were explored using nuclear ribosomal DNA internal transcribed spacers (ITS) and several ...chloroplast DNA segments. There have been few studies conducted on chloroplast genomes in
, which has limited systematic understanding of this genus. We assembled the complete chloroplast genomes of nine
species from China using data generated from next generation sequencing (NGS). The chloroplast (cp) DNA used were standard double-stranded molecules, ranging from 146,432 base pairs (bp) (
) to 165,666 bp (
) in length. The circular DNA contained a large single-copy (LSC) region, small single-copy (SSC) region, and pair of inverted repeats (IRs). The cp DNA of the nine species contained 82-93 protein-coding genes, 36-37 transfer RNA (tRNA) genes, and eight ribosomal RNA (rRNA) genes, respectively. Four species (
,
,
, and
) exhibited striking distinctions in genome size, gene number, IR boundary, and sequence identity. We confirmed the non-monophyly of the
species on the basis of the nine newly identified plastomes. The distant relationship between the above-mentioned four
species and Pimpinelleae was indicated with high support values. Our study provides a foundation for future in-depth phylogenetic and taxonomic studies of genus
.
The effect of water source on cognitive functioning is poorly understood. The present study explored the associations between water source and cognition in 9921 participants from the China Health and ...Retirement Longitudinal Study (CHARLS). Cognitive functioning was measured from three aspects: orientation and attention, episodic memory, and visuo-construction. Water sources included tap and non-tap water. Generalized linear models and multiple logistic regression models were conducted to investigate the associations of cognitive scores with water source among the whole population and different subgroups. Results from cross-sectional analysis reported that participants without access to tap water showed a lower cognitive score (β = − 0.57; 95% CI: − 0.74, − 0.39) than those with tap water as a water source; and this phenomenon was pronounced for both sexes and across all residences. During 4-years’ follow-up, a greater decline of cognitive score was associated with no tap water use in the lowest quartile of baseline cognitive scores (β = − 0.67; 95% CI: − 1.26, − 0.08). Additionally, the utilization rate of tap water was lower in rural areas compared to urban areas. The lowest utilization rates were observed in urban areas of the Anhui province (0.38 in 2011 and 0.55 in 2015) and in rural areas of Inner Mongolia (0.09 in 2011 and 0.20 in 2015). These findings suggest that having no tap water may be a risk factor for cognition impairment, particularly for those with a low basic cognition score. Additionally, our results support the need to expand tap water use in China.
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•Non-tap water users showed a declined cognition when compared to tap water users.•Non-tap water users with lower cognitive scores had faster cognitive declines.•Urban areas in Anhui province showed a lowest utilization of tap water in China.•Rural areas in Inner Mongolia showed a lowest utilization of tap water in China.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Frailty and depression, as two common conditions among older adults in China, have been shown to be closely related to each other. The aim of this study was to investigate the bidirectional effects ...between frailty and depressive symptoms in Chinese population.
The bidirectional effect of frailty with depressive symptoms was analyzed among 5,303 adults ≥ 60 years of age from the China Health and Retirement Longitudinal Study (CHARLS). Phenotype and a frailty index were used to measure frailty. Depressive symptoms were evaluated using the Epidemiological Studies Depression Scale (CES-D). Logistic regression and Cox proportional hazard regression models were used to determine the bidirectional effects of frailty and depressive symptoms in cross-sectional and cohort studies, respectively. Subgroup and sensitivity analyses were further used to further verify the associations.
In the cross-sectional study, the multivariate-adjusted ORs (95% CIs) for depressive symptoms among pre-frail and frail adults, as defined by the frailty index and phenotype, were 3.05 (2.68-3.49), and 9.78 (8.02-12.03), respectively. Depressed participants showed higher risks of pre-frailty and frailty frailty index, 3.07 (2.69-3.50); and phenotypic frailty, 9.95 (8.15-12.24). During follow-up, the multivariate-adjusted HRs (95% CIs) for depressive symptoms among pre-frail and frail participants, as defined by the frailty index and phenotype, were 1.38 (1.22-1.57), and 1.30 (1.14-1.48), respectively. No significant relationship existed between baseline depressive symptoms and the incidence of frailty. Moreover, the results from subgroup and sensitivity analyses were consistent with the main results.
Although a cross-sectional bidirectional association between depressive symptom and frailty has been observed in older (≥60 years old) Chinese adults, frailty may be an independent predictor for subsequent depression. Moreover, no effect of depressive symptoms on subsequent frailty was detected. Additional bidirectional studies are warranted in China.
is widely found in spoilage fish, shrimp and other seafoods. Under suitable conditions, ornithine can be synthesized into putrescine, which may spoil food or endanger health. Our research used a wild ...strain of
isolated from "Yi Lu Xian" salted fish (a salting method for sea bass) as a research object. According to the database of National Center of Biotechnology Information (NCBI), the target ornithine decarboxylase (ODC) gene SpeF was successfully amplified using the wild strain of
as the template. Sequencing alignment showed that the SpeF of the wild strain had more than 98% homology compared with the standard strain. The amino acid substitution occurred in the residues of 343, 618, 705, and 708 in the wild strain. After optimizing the expression conditions, a heterologous expression system of ODC was constructed to achieve a high yield of expression. The amount of 253.38 mg of ODC per liter of LB broth was finally expressed. High performance liquid chromatography (HPLC) and subsequent ODC activity verification experiments showed that hetero-expressed ODC showed a certain enzyme activity for about 11.91 ± 0.38 U/mg. Our study gives a new way to the development of a low-cost and high-yield strategy to produce ODC, providing experimental materials for further research and elimination of putrescine in food hazards.
In this work, a reusable supramolecular platform for the specific capture and release of proteins and bacteria was developed. Multilayered polyelectrolyte films containing "guest" moieties were first ...fabricated using the layer-by-layer (LbL) deposition of poly(allylamine hydrochloride) and poly(acrylic acid-co-1-adamantan-1-ylmethyl acrylate), followed by the incorporation of β-cyclodextrin (β-CD) derivatives modified with mannose (CD-M) as "host" molecules with protein (lectin) binding properties. This platform combines three different non-covalent interactions: electrostatic interactions for the LbL deposition of multilayered films, host-guest inclusion for the incorporation of β-CD-conjugated ligands, and carbohydrate-protein affinity recognition for the capture of specific proteins and bacteria. For the mannose system investigated, the capture of Concanavalin A (ConA) and type I fimbriated Escherichia coli was demonstrated. Moreover, due to the inherent reversibility of host-guest interactions, the captured proteins and bacteria could be easily released from the surface by incubation with sodium dodecyl sulfate, and the renewed "guest" surface could be treated with the CD-M "host" to regenerate the ConA and E. coli-binding surface. This "use-regenerate" cycle could be repeated multiple times without significant loss of bioactivity. Given the generality and versatility of this approach, it may provide the basis for the development of re-usable biosensors and diagnostic devices for the detection and measurement of proteins and bacteria.
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