As plantas daninhas são causa do encarecimento das lavouras, entre elas está Conyza spp, conhecida como buva. Marcadores moleculares vem sendo utilizados para complementar sua caracterização. A ...técnica AFLP (Amplified Fragment Length Polymorphism) é adaptada para diversas análises genéticas, fornecendo alta cobertura do genoma. Com objetivo de caracterizar molecularmente morfotipos de buva, foram coletadas 60 plantas da UENP – Bandeirantes/PR. A análise molecular com AFLP seguiu etapas de digestão, ligação de adaptadores, amplificação pré-seletiva e seletiva, utilizando combinação de seis pares de primers AFLP. Os fragmentos foram separados em gel de poliacrilamida 8% e corados com AgNo3. Dos 218 loci, 97 foram polimórficos sendo 53 específicos para um morfotipo e 44 para o outro. A matriz de distância genética, a porcentagem de locos polimórficos, diversidade gênica de Nei (1978), e a distância genética foram obtidas utilizando o programa TFPGA (Tools For Population Genetcs Analyses). Os resultados da AMOVA mostram que a variação entre as populações é de 85,18% e dentro das populações é de 14,82% indicando que os morfotipos sejam espécies diferentes. Através das análises moleculares e principais características morfológicas demonstrou se que os morfotipos são espécies distintas, o morfotipo A é Conyza sumatrensis e o morfotipo B Conyza bonariensis.
The diversity of
Phakopsora pachyrhizi
populations has often resulted in the selection of virulent pathotypes following the release of rust-resistant cultivars. Thus, knowledge of the pathogenicity ...and variability of
P. pachyrhizi
specie, coupled with an understanding of its biology and host-pathogen interactions, is essential for better comprehension of the evolution and rapid adaption of the pathogen. Such comprehension can be useful for genetic breeding programs and other research areas. Therefore, this study aimed to assess the genetic variability and virulence among
P. pachyrhizi
mono-uredinial isolates obtained from field samples collected in Brazil during the period from 2007 to 2012 by comparing the sequences of Internal Transcribed Spacer (ITS) regions and AFLP markers and analyzing the responses of differential soybean genotypes. The polymorphisms among the AFLP markers (locally) and ITS sequences (globally) demonstrated the high genetic diversity of this pathogen. Phenotypic analysis revealed the presence of at least 16 different pathotypes present in soybean fields in Brazil. The most effective resistance gene was
Rpp
5 (PI 200487), followed by
Rpp
4 (PI 459025) and
Rpp
2 (230970). This study represents the first report on the virulence and molecular characterization of a collection of
P. pachyrhizi
mono-uredinial isolates, which might represent different races occurring in Brazilian fields in the period from 2007 to 2012.
Full text
Available for:
EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OBVAL, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Load filters
No result was selected!
Please select the results that you wish to export.
The search was successfully saved.
Editing
The search could not be saved.
Saved searches can be viewed in the list My searches.
The changes made to the saved search were saved successfully.
Save search
Shelf entry
No result was selected!
Adding material to shelf was successful.
Adding material to shelf was partly successful.
Adding material to shelf failed completely.
It was not necessary to add the material to the shelf.
Please select the results that you want to put on shelf!
On shelf the following records have been successfully added:
On shelf the following records have been successfully added:
