p53 binding protein-1 (53BP1) participates in checkpoint signaling during the DNA damage response (DDR) and during mitosis. In this study we report that 53BP1 aggregates in nuclear foci within ...syncytia elicited by the human immunodeficiency virus (HIV)-1 envelope. 53BP1 aggregation occurs as a consequence of nuclear fusion (karyogamy (KG)). It colocalizes partially with the promyelomonocytic leukemia protein (PML), and the ataxia telangiectasia mutated kinase (ATM), the two components of the DDR that mediate apoptosis induced by the HIV-1 envelope. ATM-dependent phosphorylation of 53BP1 on serines 25 and 1778 (53BP1S25P and 53BP1S1778P) occurs at these DNA damage foci. 53BP1S25P was also detected in syncytia present in the lymph nodes or frontal brain sections from HIV-1-infected carriers, as well as in peripheral blood mononucleated cells from HIV-1-infected individuals, correlating with viral load. Knockdown of 53BP1 caused HIV-1 envelope-induced syncytia to enter abnormal mitoses, leading to their selective destruction through mitochondrion-dependent and caspase-dependent pathways. In conclusion, depletion of 53BP1 triggers the demise of HIV-1-elicited syncytia through mitotic catastrophe.
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DOBA, EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, IZUM, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UILJ, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
We report a case of erythema annulare centrifugum present since birth in three generations in an Albanian family. The proband is a 45-year-old white Caucasian man; his mother (deceased), a ...38-year-old sister and a 16-year-old niece presented with the same phenotype. To our knowledge this is the third case of familial annular erythema since its original description in 1966 by Beare et al. Autosomal dominant inheritance of the disorder is suggested.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
Introduction Becoming aware of being HCV positive has a very negative impact on quality of life and IFN therapy is also responsible for the development of neuropsychiatric side effects (Pariante ...2002). Objective This prospective study aims to determine the role of IFN in psychopathological symptoms experienced by patients in treatment, in particular it deals with its impact on quality of life and determines which patients can be safely treated with Peg-interferon α, because the therapy is expensive, has significant side effects and prolonged psychiatric that unfortunately cannot be predicted with absolute certainty. Methods The sample includes 32 patients, including 18 males and 14 females of mean age of 48.19 years (DS. = 9.660) hepatitis C affected, evaluated before the start of peg-interferon antiviral therapy using the interview semi-structured SCID-I and II and self-administered tests Scl90, BDI, SF36 and followed up at first and third months by HAM-a, HAM-D, CGI, YMRS, and sixth months of therapy through Scl90, BDI, SF36, HAM-A, HAM-D, CGI, YMRS. Conclusions Although psychiatric disorders worsened or manifested during the peg-interferon α + ribavirin therapy, only 2 out of 32 patients did not come to the end point of our study because of a psychopathological disease. Despite the predominantly psychopathological depressive symptoms and the marked quality of life reduction, with adequate support, even patients predisposed to mood disorders or anxiety, can take the peg-interferon α, and the presence of a life-time psychiatric diagnosis is not an a priori therapy exclusion criterion.
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GEOZS, IMTLJ, KILJ, NUK, OILJ, PNG, SBJE, UL, UM, UPUK
•Exhausted myrtle berries (EMB) could be a source of bioactive compounds: polyphenols.•The EMB fed to sheep affect the rumen microbiota structure.•The EMB included in diet sheep reduced abundance of ...Butyrivibrio group.•The EMB included in sheep diet decreased the rumen ammonia accumulation.•The EMB improved the dietary nitrogen utilization at rumen level.
Manipulation of the gut functions, and rumen microbiota and metabolism with feed additives, in particular those rich in polyphenols, has been recognized as promising strategy for improving feed efficiency in sheep. Aiming to evaluate the effect of the dietary supplementation of exhausted myrtle berries (EMB), as source of polyphenols, on the metabolism and microbial population in ruminal fluid of sheep, eighteen Sarda dairy ewes were used in a 11-week experiment. Ewes were allocated to three groups, homogeneous for milk production, days in milking and parity. The groups were assigned to three diets, which were individually fed: i) a control diet (CON); ii) a diet supplemented with 50 g/d per animal of EMB (EMB50); iii) a diet supplemented with 100 g/d per animal of EMB (EMB100). The basal diet (the same for all animals) consisted of dehydrated alfalfa (1.2 kg/d per animal), a commercial concentrate (0.50 kg/d per animal), a mixed meal (soybean, corn, and linseed, 0.15, 0.10 and 0.20 kg/d per animal, respectively) and beet pulp (0.25 kg/d per animal). The supplementation with EMB did not affect (P > 0.05) the rumen pH. Ammonia content was reduced (P < 0.05) by the EMB supplementation at both doses of EMB compared with CON. The total volatile fatty acids (VFA) decreased (P < 0.05) in EMB100 compared to CON and EMB50, whereas the molar proportions of acetate, propionate, butyrate and the ratio acetate:propionate were not affected by the diet. Diet did not affect (P > 0.05) the estimated production of methane. Weak effects were observed on ruminal concentration fatty acids (FA), except for the CLAcis-9,trans-11 and total CLA concentrations, that increased (P < 0.05) in EMB50 and tended to increase (P < 0.10) in EMB100 compared with CON. The abundances of the microbial groups studied in this work were, in general, influenced by the diet × sampling date interaction. The low dose of EMB increased (P < 0.05) the abundance of fungi. Butyrivibrio group was significantly lower (P < 0.05) in both EMB50 and EMB100 diets than in CON. Overall, the reduction of rumen ammonia accumulation, which could reflect the reduction of Butyrivibrio group, known as proteolytic bacteria, suggests that EMB, as source of polyphenols, might be useful to improve nitrogen balance in dairy sheep.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
38.
Does HLA-G play a role in PBC? Miglianti, M.; Littera, R.; Mocci, S. ...
Digestive and liver disease,
02/2024, Volume:
56
Journal Article
Peer reviewed
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
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