The internal transcribed spacer (ITS) region of the nuclear rDNA cistrons is one of the more frequently utilized regions for phylogenetic analyses at the genus and species levels. It has proven ...valuable for phylogenetic reconstruction of species and genus relationships, using comparisons of primary sequence. Although potential transcript secondary structure homology is often utilized to aid alignment in comparisons of ribosomal gene sequences, such consideration has rarely been applied to ITS primarily because secondary structures for its transcript were not available. Here, we describe the value of applying ITS2 RNA transcript secondary structure information to improve alignments, which then allows comparisons at even deeper taxonomic levels. The evolutionarily conserved subportions of ITS2, apparently necessary for positioning of the multimolecular transcript processing machinery, also provide material for distinguishing evolutionarily rare events, ‘Compensatory Base Changes’ in the relatively conserved regions, that might be useful in recognizing how arbitrary are the assignments of classical taxonomic ranks in disparate groups of eukaryotes. This relatively short and easily sequenced region of DNA – ITS2 – has yet to be fully exploited in phylogenetics.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
The biochemistry of the para-sulfonato-calixnarenes has shown rapid development during the past ten years, the highly diverse biomedical applications of these molecules now include anti-viral, ...anti-thrombotic activities, enzyme blocking and protein complexation. The future is even more promising as para-sulfonato-calixnarenes have, now, been shown to have potential in the diagnosis of prion-based diseases. Their innocuous nature, as far as is known at present, may open up their future use in medications.
In Understanding Edward P. Jones, James W. Coleman analyzes Jones’s award-winning works as well as the significant influences that have shaped his craft. Born and raised in Washington, D.C., Jones ...has made that city and its African American community the subject of or background for most of his fiction. Though Jones’s first work was published in 1976, his career developed slowly. While he worked for two decades as a proofreader and abstractor, Jones published short fiction in such periodicals as Essence, the New Yorker, and Paris Review. His first collection, Lost in the City, won the PEN/Hemingway Award, and subsequent books, including The Known World and All Aunt Hagar’s Children, received similar accolades, including the National Book Critics Circle Award and the Pulitzer Prize for Fiction. Following an overview of Jones’s life, influences, and career, Coleman provides an introduction to the technique of Jones’s fiction, which he likens to a tapestry, woven of intricate, varied, and sometimes disparate elements. He then analyzes the formal structure, themes, and characters of The Known World and devotes a chapter each to the short story collections Lost in the City and All Aunt Hagar’s Children. His discussion of these volumes focuses on Jones’s narrative technique; the themes of family, community, and broader tradition; and the connections through which the stories in each volume collectively create a thematic whole. In his final chapter, Coleman assesses Jones’s encompassing outlook that sees African American life in distinct periods but also as a historical whole, simultaneously in the future, the past, and the present.
BACKGROUNDCellulite refers to the dimpled appearance of skin occurring where the dermis is tethered by subcutaneous fibrous septa arranged perpendicularly to the skin surface.
OBJECTIVETo demonstrate ...the safety and efficacy of a new system for vacuum-assisted precise tissue release for the treatment of cellulite.
MATERIALS AND METHODSAdult women with moderate to severe cellulite (N = 55) underwent a single treatment. Post-treatment assessments were performed after 3 and 14 days; 1, 3, and 6 months; and at 1 year. Outcome measures included blinded assessments of subject photographs, a validated Cellulite Severity Scale (CSS), and the Global Aesthetic Improvement Scale. Subject satisfaction and pain ratings were also recorded.
RESULTSThe mean baseline CSS score of 3.4 decreased to 1.3 at 3 months (p < .0001) and 1.4 at 1 year (p < .0001), with 47 subjects (93%) having ≥1-point improvements. Subject satisfaction was 85% at 3 months and 94% at 1 year. Transient treatment-related adverse events were mild in severity.
CONCLUSIONThis study demonstrates the safety, efficacy, and subject satisfaction with vacuum-assisted precise tissue release in the treatment of cellulite. There was no reduction in treatment benefits for up to 1 year. These results supported the Food and Drug Administration clearance of the device for the long-term reduction in the appearance of cellulite.
Nuclear rDNA Internal Transcribed Spacers, ITS1 and ITS2, are widely used for eukaryote phylogenetic studies from the ordinal level to the species level, and there is even a database for ITS2 ...sequences. However, ITS regions have been ignored in mammalian phylogenetic studies, and only a few rodent and ape sequences are represented in GenBank. The reasons for this dearth, and the remedies, are described here. We have recovered these sequences, mostly >1 kb in length, for 36 mammalian species. Sequence alignment and transcript folding comparisons reveal the rRNA transcript secondary structure. Mammalian ITS regions, though quite long, still fold into the recognizable secondary structure of other eukaryotes. The ITS2 in particular bears the four standard helix loops, and loops II and III have the hallmark characters universal to eukaryotes. Both sequence and insertions/deletions of transcript secondary structure helices observed here support the four superorder taxonomy of Placentalia. On the family level, major unique indels, neatly excising entire helices, will be useful when additional species are represented, resulting in significant further understanding of the details of mammalian evolutionary history. Furthermore, the identification of a highly conserved element of ITS1 common to warm-blooded vertebrates may aid in deciphering the complex mechanism of RNA transcript processing. This is the last major group of terrestrial vertebrates for which rRNA ITS secondary structure has been resolved.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
The International Campaign for Cures of Spinal Cord Injury Paralysis (ICCP) supported an international panel tasked with reviewing the methodology for clinical trials in spinal cord injury (SCI), and ...making recommendations on the conduct of future trials. This is the first of four papers. Here, we examine the spontaneous rate of recovery after SCI and resulting consequences for achieving statistically significant results in clinical trials. We have reanalysed data from the Sygen trial to provide some of this information. Almost all people living with SCI show some recovery of motor function below the initial spinal injury level. While the spontaneous recovery of motor function in patients with motor-complete SCI is fairly limited and predictable, recovery in incomplete SCI patients (American spinal injury Association impairment scale (AIS) C and AIS D) is both more substantial and highly variable. With motor complete lesions (AIS A/AIS B) the majority of functional return is within the zone of partial preservation, and may be sufficient to reclassify the injury level to a lower spinal level. The vast majority of recovery occurs in the first 3 months, but a small amount can persist for up to 18 months or longer. Some sensory recovery occurs after SCI, on roughly the same time course as motor recovery. Based on previous data of the magnitude of spontaneous recovery after SCI, as measured by changes in ASIA motor scores, power calculations suggest that the number of subjects required to achieve a significant result from a trial declines considerably as the start of the study is delayed after SCI. Trials of treatments that are most efficacious when given soon after injury will therefore, require larger patient numbers than trials of treatments that are effective at later time points. As AIS B patients show greater spontaneous recovery than AIS A patients, the number of AIS A patients requiring to be enrolled into a trial is lower. This factor will have to be balanced against the possibility that some treatments will be more effective in incomplete patients. Trials involving motor incomplete SCI patients, or trials where an accurate assessment of AIS grade cannot be made before the start of the trial, will require large subject numbers and/or better objective assessment methods.
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DOBA, EMUNI, FIS, FSPLJ, FZAB, GEOZS, GIS, IJS, IMTLJ, IZUM, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, SIK, UILJ, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Patterns of multiple paternity and maternity in fishes COLEMAN, SETH W; JONES, ADAM G
Biological Journal of the Linnean Society/Biological journal of the Linnean Society,
August 2011, Volume:
103, Issue:
4
Journal Article
Group 1 pulmonary arterial hypertension (PAH) is a rare disease with high mortality despite recent therapeutic advances. Pathogenic remodeling of pulmonary arterioles leads to increased pulmonary ...pressures, right ventricular hypertrophy, and heart failure. Mutations in bone morphogenetic protein receptor type 2 and other risk genes predispose to disease, but the vast majority of non-familial cases remain genetically undefined.
To identify new risk genes, we performed exome sequencing in a large cohort from the National Biological Sample and Data Repository for PAH (PAH Biobank, n = 2572). We then carried out rare deleterious variant identification followed by case-control gene-based association analyses. To control for population structure, only unrelated European cases (n = 1832) and controls (n = 12,771) were used in association tests. Empirical p values were determined by permutation analyses, and the threshold for significance defined by Bonferroni's correction for multiple testing.
Tissue kallikrein 1 (KLK1) and gamma glutamyl carboxylase (GGCX) were identified as new candidate risk genes for idiopathic PAH (IPAH) with genome-wide significance. We note that variant carriers had later mean age of onset and relatively moderate disease phenotypes compared to bone morphogenetic receptor type 2 variant carriers. We also confirmed the genome-wide association of recently reported growth differentiation factor (GDF2) with IPAH and further implicate T-box 4 (TBX4) with child-onset PAH.
We report robust association of novel genes KLK1 and GGCX with IPAH, accounting for ~ 0.4% and 0.9% of PAH Biobank cases, respectively. Both genes play important roles in vascular hemodynamics and inflammation but have not been implicated in PAH previously. These data suggest new genes, pathogenic mechanisms, and therapeutic targets for this lethal vasculopathy.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
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