The seasonal dynamics of the total flavonoid contents in various parts of Dryopteris erythrosora, a traditional Chinese medicinal fern, and their antioxidant activity were investigated. The total ...flavonoids content in various parts of D. erythrosora showed an obvious seasonal dynamic change. The total flavonoid contents in stems (from 4.3% to 12.5%) were much higher than that in leaves with an average content of 2.01%. In spring, the total flavonoid contents in stems were relatively low, but increased rapidly from summer to winter. However, the seasonal dynamics of total flavonoid contents in leaves showed different model. The total flavonoid contents in the stems showed a negative correlation with that in the leaves from January to July. The correlation coefficient of about -0.7 was obtained. The antioxidant activity of the extracts also altered in proportion to the change of total flavonoid contents. In general, the extracts from stems always showed highest antioxidant potentials and it was suggested that the stems can be used as crude medicine.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
The flavonoids in bryophytes may have great significance in phylogeny and metabolism research. However, to date there has been little research on bryophyte metabolites, especially flavonoids. To ...redress this somewhat, we determined flavonoid concentrations of bryophytes from Tianmu Mountain through a colorimetric assay and considered the factors influencing the results. This is the first time that the flavonoid contents of bryophytes have been examined in detail. The results revealed a range of total flavonoid concentrations in 90 samples collected from Tianmu Mountain from 1.8 to 22.3 mg/g (w/w). The total flavonoid contents of liverworts were generally higher than those of mosses; acrocarpous mosses had generally higher values than that of pleurocarpous mosses. The total flavonoid contents of bryophytes growing at lower light levels were general higher than those growing in full-sun. The total flavonoid contents of epiphytic bryophytes were highest, while those of aquatic bryophytes were the lowest. Total flavonoid contents of species growing at low-latitudes were much higher than those at high-latitude individuals. In conclusion, total flavonoid contents of bryophytes have some connection with plant phylogeny; more flavonoids might be contained in relatively primitive bryophytes. Meanwhile, the effects of ecological factors on total flavonoid contents of bryophytes exist; light and habitat (especially tree habitat and river habitat) might be representative factor.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
New 2,3-disubstituted thieno2,3-
pyrimidin-4(3
)-ones were synthesized via a one-pot reaction from 2
-thieno2,3-
1,3oxazine-2,4(1
)-diones, aromatic aldehydes, and benzylamine or ...4-hydroxylbezylamine. The obtained compounds were tested in vitro for cancer cell growth inhibition. Compound
can inhibit all four types of tested cancer cells, i.e., MCF-7, A549, PC-9, and PC-3 cells. Most of the compounds inhibited the proliferation of A549 and MCF-7 cells. Compound
exhibited the strongest anti-proliferative effect against A549 cell lines with IC
values of 0.94 μM, and with no toxicity to normal human liver cells. Its potency was further proved by cell clone formation assay, Hoechst 33258 staining, and evaluation on the effects of apoptosis-related proteins.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
Hemionitis albofusca
(Baker) Christenh is a plant that grows in various regions of China. Although it is not recognized as a traditional medicine, it is often mistakenly labelled and used as
...Aleuritopteris argentea
(S. G. Gmél.) Fée to alleviate menstruation-related issues. Recently, several diterpenoids such as
ent
-16-oxo-17-norkauran-19-oic acid (Compound
A
), 14-oxy-7β,20-dihydroxycyath-12,18-diene (Compound
B
),
ent
-8(14),15-pimaradiene-2β,19-diol (Compound
C
),
ent
-kaurane-16-ene-2β,18α-diol (Compound
D
),
ent
-kaurane-2β,16α,18α-triol (Compound
E
), and onychiol B have been extracted from
H. albofusca
. In this study, we investigated the anti-inflammatory activity of these diterpenes. We confirmed that compounds
A
~
D
suppressed the amount of cellular NO production by inhibiting the expression and transcription of iNOS protein. They also significantly inhibited the expression and transcription of inflammatory factors TNF-α and IL-6. Additionally, Compounds
A
and
C
suppressed the activation of the NF-κB signaling pathway and inhibited the phosphorylation level of p38, ultimately down-regulating inflammation. Compound
B
suppressed the activation of the NF-κB signaling pathway, while Compound
D
inhibited the phosphorylation level of p38 and down-regulated the activation of the p38 MAPK signaling pathway. In a word, our investigation supports the potential application of natural diterpenes as lead compounds for developing anti-inflammatory agents.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Cu
2
O/TiO
2
visible-light photocatalytic composite was successfully synthesized by supercritical solvothermal route. Cu
2
O/TiO
2
presented excellent bacterial inactivation activity for
Pseudomonas ...marginalis
pv. marginalis, which was related to the concentration of bacteria and the antibacterial time. The highest sterilization ratio reached up to 100% when the bacteria was treated with 80 μg/mL of Cu
2
O/TiO
2
photocatalytic composite for 80 min, which could be further proved by the damage of integrity and shrink of the cell membrane in transmission electron microscopy (TEM) image. When the bacterial concentration was 1 × 10
5
CFU/mL, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined as 16 and 32 μg/mL by agar dilution, respectively. Meanwhile, the production of reactive oxygen species (ROS), glutathione reductase (GR) and glutathione (GSH) of
Pseudomonas marginalis
pv. marginalis treated by Cu
2
O/TiO
2
were determined by DCFH-DA, DTNB and kinetic method, respectively, to evaluate the anti-oxidation capacity of bacteria cell. The enzyme activity of peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) in bacteria treated with Cu
2
O/TiO
2
were measured to further confirm the overproduction of ROS. Cu
2
O/TiO
2
was demonstrated as the excellent visible-light photocatalyst for efficiently killing
Pseudomonas marginalis
pv. marginalis with the low dosage. Finally, the Cu
2
O/TiO
2
composite photocatalytic material was applied to cucumber seedlings based on field experimental, and its inhibitory effect in practical application was judged by measuring the morphology, enzyme activity and resistance index of cucumber plants. It is of great significance to the practical application as a suitable and powerful antibacterial agent for
Pseudomonas marginalis
pv. marginalis and other bacteria.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
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Ancepsone A, a new sesterterpene with 13,19-epoxycheilanthane skeleton, i.e (17Z)-13,19-epoxycheilanth-1,17-diene-2-ol-3-one (1), together with eight known natural products (2 ∼ 9) ...were isolated from Aleuritopteris anceps. The structure of the new compound was established by means of 1D and 2D NMR, MS, and single-crystal X-ray diffraction methods. All compounds were isolated from A. anceps for the first time. Ancepsone A and the isolated flavonoids can moderately inhibit the proliferation of cancer cells.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Twenty compounds were isolated from the fern of Pyrrosia davidii (Baker) Ching. These compounds included three steroids (1, 2, and 3), one pigment (4), eleven flavonoids (5, 6, 7, 8, 9, 10, 11, 12, ...13, 14, and 15), two terpenoids (16, 17), one phenolic acid (18), and two fatty acids (19, 20). The structures of the compounds were determined by comparison of measured NMR spectroscopic data with previously reported data. Except for Compound 1 and 11, all the compounds were isolated for the first time from Pyrrosia davidii (Baker) Ching, and Compounds 6, 9, 17, 19, and 20 were isolated for the first time from the genus Pyrrosia Mirbel. The chemotaxonomic significance of these compounds was discussed.
•Eighteen compounds were isolated from the fern of Pyrrosia davidii for the first time.•Compounds 6, 9, 17, 19, and 20 were isolated from the genus Pyrrosia Mirbel for the first time.•The chemotaxonomic significance of these isolates was discussed.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
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