Dogs offer unique opportunities to study correlations between morphology and behavior because skull shapes and body shape are so diverse among breeds. Several studies have shown relationships between ...canine cephalic index (CI: the ratio of skull width to skull length) and neural architecture. Data on the CI of adult, show-quality dogs (six males and six females) were sourced in Australia along with existing data on the breeds' height, bodyweight and related to data on 36 behavioral traits of companion dogs (n = 8,301) of various common breeds (n = 49) collected internationally using the Canine Behavioral Assessment and Research Questionnaire (C-BARQ). Stepwise backward elimination regressions revealed that, across the breeds, 33 behavioral traits all but one of which are undesirable in companion animals correlated with either height alone (n = 14), bodyweight alone (n = 5), CI alone (n = 3), bodyweight-and-skull shape combined (n = 2), height-and-skull shape combined (n = 3) or height-and-bodyweight combined (n = 6). For example, breed average height showed strongly significant inverse relationships (p<0.001) with mounting persons or objects, touch sensitivity, urination when left alone, dog-directed fear, separation-related problems, non-social fear, defecation when left alone, owner-directed aggression, begging for food, urine marking and attachment/attention-seeking, while bodyweight showed strongly significant inverse relationships (p<0.001) with excitability and being reported as hyperactive. Apart from trainability, all regression coefficients with height were negative indicating that, across the breeds, behavior becomes more problematic as height decreases. Allogrooming increased strongly (p<0.001) with CI and inversely with height. CI alone showed a strong significant positive relationship with self-grooming (p<0.001) but a negative relationship with chasing (p = 0.020). The current study demonstrates how aspects of CI (and therefore brain shape), bodyweight and height co-vary with behavior. The biological basis for, and significance of, these associations remain to be determined.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Context:
The FSH receptor (FSHR) is traditionally thought to play a role in female reproductive physiology solely within the context of ovarian FSHR. However, FSHR is also expressed in endothelial ...cells of the placental vasculature and human umbilical cord vessels, suggesting additional facets of female reproduction regulated by extragonadal FSHR.
Objective:
We sought to determine the functional role of FSHR on human umbilical cord endothelial cells (HUVECs), hypothesizing that activation of the FSHR would stimulate angiogenesis.
Design:
The ability of FSH to stimulate several angiogenic processes in HUVECs was determined.
Setting:
This was a laboratory-based study using commercially prepared HUVECs.
Results:
Tube formation, wound healing, cell migration, cell proliferation, nitric oxide production, and cell survival were stimulated in response to FSH. Quantitative comparisons between HUVECs incubated with maximally stimulatory concentrations of FSH vs vascular endothelial growth factor (VEGF), a well-characterized angiogenic factor, revealed that FSH is as efficacious as VEGF in promoting angiogenic processes. FSH did not provoke increased secretion of VEGF by HUVECs, suggesting the direct stimulation of angiogenic processes by FSH in endothelial cells. In contrast to gonadal cells, the FSHR on HUVECs did not mediate an FSH-stimulated increase in cAMP. However, increased phosphorylation of AKT in response to FSH was observed, suggesting that FSH stimulation of HUVEC FSHR stimulates the PI3K/AKT signaling pathway.
Conclusions:
Our studies reveal a novel role for FSHR in female reproductive physiology. Its ability to promote angiogenesis in placental endothelial cells suggests that the FSHR may have an influential role in pregnancy.
Breed differences in canine aggression Duffy, Deborah L.; Hsu, Yuying; Serpell, James A.
Applied animal behaviour science,
12/2008, Volume:
114, Issue:
3
Journal Article
Peer reviewed
Canine aggression poses serious public health and animal welfare concerns. Most of what is understood about breed differences in aggression comes from reports based on bite statistics, behavior ...clinic caseloads, and experts’ opinions. Information on breed-specific aggressiveness derived from such sources may be misleading due to biases attributable to a disproportionate risk of injury associated with larger and/or more physically powerful breeds and the existence of breed stereotypes. The present study surveyed the owners of more than 30 breeds of dogs using the Canine Behavioral Assessment and Research Questionnaire (C-BARQ), a validated and reliable instrument for assessing dogs’ typical and recent responses to a variety of common stimuli and situations. Two independent data samples (a random sample of breed club members and an online sample) yielded significant differences among breeds in aggression directed toward strangers, owners and dogs (Kruskal–Wallis tests,
P
<
0.0001).
Eight breeds common to both datasets (Dachshund, English Springer Spaniel, Golden Retriever, Labrador Retriever, Poodle, Rottweiler, Shetland Sheepdog and Siberian Husky) ranked similarly,
r
s
=
0.723,
P
<
0.05;
r
s
=
0.929,
P
<
0.001;
r
s
=
0.592,
P
=
0.123, for aggression directed toward strangers, dogs and owners, respectively. Some breeds scored higher than average for aggression directed toward both humans and dogs (e.g., Chihuahuas and Dachshunds) while other breeds scored high only for specific targets (e.g., dog-directed aggression among Akitas and Pit Bull Terriers). In general, aggression was most severe when directed toward other dogs followed by unfamiliar people and household members. Breeds with the greatest percentage of dogs exhibiting serious aggression (bites or bite attempts) toward humans included Dachshunds, Chihuahuas and Jack Russell Terriers (toward strangers and owners); Australian Cattle Dogs (toward strangers); and American Cocker Spaniels and Beagles (toward owners). More than 20% of Akitas, Jack Russell Terriers and Pit Bull Terriers were reported as displaying serious aggression toward unfamiliar dogs. Golden Retrievers, Labradors Retrievers, Bernese Mountain Dogs, Brittany Spaniels, Greyhounds and Whippets were the least aggressive toward both humans and dogs. Among English Springer Spaniels, conformation-bred dogs were more aggressive to humans and dogs than field-bred dogs (stranger aggression: Mann–Whitney
U test,
z
=
3.880,
P
<
0.0001; owner aggression:
z
=
2.110,
P
<
0.05; dog-directed aggression:
z
=
1.93,
P
=
0.054), suggesting a genetic influence on the behavior. The opposite pattern was observed for owner-directed aggression among Labrador Retrievers, (
z
=
2.18,
P
<
0.05) indicating that higher levels of aggression are not attributable to breeding for show per se.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
Objective-To compare the owner-reported prevalence of behavioral characteristics in dogs obtained as puppies from pet stores with that of dogs obtained as puppies from noncommercial breeders. ...Design-Cross-sectional study. Animals-Dogs obtained as puppies from pet stores (n = 413) and breeder-obtained dogs (5,657). Procedures-Behavioral evaluations were obtained from a large convenience sample of current dog owners with the online version of the Canine Behavioral Assessment and Research Questionnaire, which uses ordinal scales to rate either the intensity or frequency of the dogs' behavior. Hierarchic linear and logistic regression models were used to analyze the effects of source of acquisition on behavioral outcomes when various confounding and intervening variables were controlled for. Results-Pet store-derived dogs received significantly less favorable scores than did breeder-obtained dogs on 12 of 14 of the behavioral variables measured; pet store dogs did not score more favorably than breeder dogs in any behavioral category. Compared with dogs obtained as puppies from noncommercial breeders, dogs obtained as puppies from pet stores had significantly greater aggression toward human family members, unfamiliar people, and other dogs; greater fear of other dogs and nonsocial stimuli; and greater separation-related problems and house soiling. Conclusions and Clinical Relevance-Obtaining dogs from pet stores versus noncommercial breeders represented a significant risk factor for the development of a wide range of undesirable behavioral characteristics. Until the causes of the unfavorable differences detected in this group of dogs can be specifically identified and remedied, the authors cannot recommend that puppies be obtained from pet stores.
•A novel questionnaire instrument for the quantitative behavioral assessment of pet cats by their owners was developed.•A convenience sample of 2068 cat owners completed an initial online survey ...regarding their cats’ behavior.•Twenty-three behavioral factors/subscales were extracted by exploratory factor analysis.•Seventeen of these 23 factors displayed adequate-high internal reliability.•The majority of factors/items also demonstrated adequate construct validity.
A questionnaire instrument for obtaining quantitative behavioral evaluations of pet cats from cat owners was developed and validated. Exploratory Factor Analysis of 2608 questionnaire responses to 149 behavioral questions/items extracted a total of 23 distinct factors that measured most of the more common dimensions of cat behavior. Seventeen of the 23 factors demonstrated adequate–high internal reliability (Cronbach’s alpha=0.712–0.923). Questionnaire validation was accomplished by determining: (a) whether owners’ subjective ratings of the severity of their cat’s behavior problems were associated with cats’ actual scores on expected questionnaire factors, (b) whether expected associations between specific demographic and/or lifestyle characteristics and behavior were confirmed by cats’ factor or item scores on the questionnaire, and (c) whether breed rankings based on owner-reported factor scores matched those previously derived from the opinions of experts (veterinarians). The results of these various tests confirmed the overall construct validity of the questionnaire.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
Gonadectomy is widely used to treat and prevent behavior problems including the aggressive behavior of dogs. The aim of this study was to determine whether aggressive behavior toward familiar people, ...strangers, or other dogs was significantly different in dogs gonadectomized at various ages vs. intact dogs using the Canine Behavioral Assessment Research Questionnaire (C-BARQ) with multivariate analysis. Of 15,370 initial surveys, those for dogs reported to have been gonadectomized at less than 6 weeks of age or to correct a behavior problem, and those with incomplete answers to questions regarding independent or dependent variables were excluded, leaving 13,795 for the analysis of aggressive behavior toward familiar people: 13,498 for aggressive behavior toward strangers and 13,237 for aggressive behavior toward dogs. Aggressive behavior was defined (a) using mean scores for all questions on the C-BARQ for aggressive behavior (range 0-4) and (b) comparing dogs with no aggressive behavior (all questions answered 0) to dogs with moderate or severe aggression (at least one score of 2, 3, or 4). Data for intact dogs were compared with those for dogs gonadectomized at 6 months or less, 7-12 months, 11-18 months, and >18 months. Neither gonadectomy nor age at gonadectomy showed an association with aggression toward familiar people or dogs. However, there was a low but significant increase in the odds of moderate or severe aggression toward strangers for all gonadectomized dogs compared with intact dogs, but this effect was driven entirely by data for dogs gonadectomized at 7-12 months of age, which were 26% more likely to demonstrate aggression toward strangers. This large, comprehensive study of the relationships between gonadectomy and aggressive behavior in dogs demonstrates that when the many factors affecting aggressive behavior are considered, there is no evidence that gonadectomy at any age alters aggressive behavior toward familiar people or dogs, and there is only a minimal increase in aggression toward strangers. Given the increasing evidence of significant negative health effects of gonadectomy, there is an urgent need to systematically examine other means of preventing unwanted procreation, such as vasectomy and hysterectomy.
The goal of the present study was to evaluate a shortened, 42-item version of the Canine Behavioral Assessment and Research Questionnaire (C-BARQ(S)) as a behavioral screening tool for dogs ...relinquished to animal shelters. In contrast to a previous finding, the current study found no consistent evidence that relinquishing owners gave unreliable or biased responses to the questionnaire depending on whether or not they believed that this information would be shared with shelter staff or used to evaluate dogs for adoption. Relinquishing owners’ C-BARQ(S) responses for items related to aggression and fear directed toward humans and other dogs correlated with independent subjective assessments of aggressiveness made by shelter staff (generalized linear mixed models, P<0.05, N=84 aggressive dogs, N=156 non-aggressive dogs). In addition, C-BARQ(S) scores successfully discriminated between dogs based on their eventual outcomes (i.e., adoption or euthanasia; Generalized Linear Mixed Models, P<0.05, N=181 adopted, N=177 euthanized), indicating convergent validity. Follow-up surveys with a subset of adoptive owners (N=53) revealed significant correlations (after correction for multiple comparisons) between relinquishing and adoptive owners’ C-BARQ(S) ratings for 3 behavioral traits: stranger-directed aggression (rs=0.494, P<0.001), chewing inappropriate objects (rs=0.402, P<0.01), and urination when left alone (rs=0.421, P<0.01). Overall, the findings confirmed the value of this type of shelter intake survey instrument for screening owner-surrendered dogs for the presence of behavior problems.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
One of the primary assumptions of the immunocompetence hypothesis is that testosterone is immunosuppressive. Although many studies in birds and mammals have supported this assumption, conflicting ...results have been reported in a variety of species. We investigated the effects of testosterone manipulation on both cell-mediated and humoral immunity in adult songbirds, European starlings (Sturnus vulgaris). Male and female starlings were wild-caught, housed in the laboratory, and implanted with either empty silastic capsules or capsules containing testosterone. Six weeks after implantation, humoral immunity was assessed by injecting the birds with a novel antigen, keyhole limpet hemocyanin, and measuring specific antibody responses 10 and 15 days later via an enzyme-linked immunosorbant assay. Cell-mediated immunity was assessed 7 weeks after implantation via intradermal injection of the T-cell mitogen phytohemagglutinin into the wing web and measuring the degree of swelling 24 h later. Antibody responses to antigenic challenge were significantly suppressed in testosterone-treated females 10 days post-injection and in both sexes 15 days post-injection. Furthermore, there was a significant inverse relationship between individual variability in antibody responsiveness and plasma testosterone concentrations. Cell-mediated responses to phytohemagglutinin stimulation were also significantly suppressed in testosterone-treated males compared to same-sex controls. Testosterone treatment significantly increased plasma corticosterone concentrations compared to controls, and the possibility of this effect mediating the immunosuppressive effects of testosterone is discussed. The present study is among the first to demonstrate testosterone-induced suppression of both cell-mediated and humoral immunity in a species of songbird.
Assistance dogs can greatly improve the lives of people with disabilities. However, a large proportion of dogs bred and trained for this purpose are deemed unable to successfully fulfill the ...behavioral demands of this role. Often, this determination is not finalized until weeks or even months into training, when the dog is close to 2 years old. Thus, there is an urgent need to develop objective selection protocols that can identify dogs most and least likely to succeed, from early in the training process. We assessed the predictive validity of two candidate measures employed by Canine Companions for Independence (CCI), a national assistance dog organization headquartered in Santa Rosa, CA. For more than a decade, CCI has collected data on their population using the Canine Behavioral Assessment and Research Questionnaire (C-BARQ) and a standardized temperament assessment known internally as the In-For-Training (IFT) test, which is conducted at the beginning of professional training. Data from both measures were divided into independent training and test datasets, with the training data used for variable selection and cross-validation. We developed three predictive models in which we predicted success or release from the training program using C-BARQ scores (
= 3,569), IFT scores (
= 5,967), and a combination of scores from both instruments (
= 2,990). All three final models performed significantly better than the null expectation when applied to the test data, with overall accuracies ranging from 64 to 68%. Model predictions were most accurate for dogs predicted to have the lowest probability of success (ranging from 85 to 92% accurate for dogs in the lowest 10% of predicted probabilities), and moderately accurate for identifying the dogs most likely to succeed (ranging from 62 to 72% for dogs in the top 10% of predicted probabilities). Combining C-BARQ and IFT predictors into a single model did not improve overall accuracy, although it did improve accuracy for dogs in the lowest 20% of predicted probabilities. Our results suggest that both types of assessments have the potential to be used as powerful screening tools, thereby allowing more efficient allocation of resources in assistance dog selection and training.
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