In this study, the impacts of egg consumption on mice model of metabolic syndrome (MetS) were comparatively investigated. Mice were divided into five groups (n=8): normal diet group (ND), high-fat ...diet group (HFD), HFD with whole egg group (WE), HFD with free-yolk egg substitute group (YFES), and HFD with lovastatin group (Lov). Main biochemical indexes and a non-targeted lipidomic analysis were employed to insight the lipid profile changes in serum. It was revealed that WE could significantly improve serum biochemical indexes by reducing body weight, low-density lipoprotein cholesterol (LDL-C) and total cholesterol (TC), while increasing high-density lipoprotein cholesterol. YFES exhibited remarkably better performance in increasing phosphatidylglycerol and phosphatidic acids, while decreasing phosphatidylinositol than WE. A total of 50 differential lipids biomarkers tightly related to glycerophospholipids metabolism were screened out. Carnitine C18:2 and C12:1, SM (d18:0/12:0), and SM (d18:1/14:1) were significantly upregulated in YFES compared to WE. YFES reduced expression of SREBP-1c and Cpt1a, while did not affect the expression of PPAR-α. Sphingomyelin biomarkers were positively related to the TC (|r| > 0.6), while PPAR-α was negatively correlated with triglyceride and LDL-C levels. To sum up, YFES attenuated HFD-induced MetS by improving the serum phospholipids, which account for its modulation of glycerophospholipid metabolism.
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•WE significantly reduced body weight, LDL-C and TC, while increased HDL-C.•YFES increased PG and PA, while decreased PI than WE.•A total of 50 differential lipids involved in glycerophospholipids metabolism were screened out.•CAR C18:2 and C12:1, SM (d18:0/12:0), and SM(d18:1/14:1) were significantly upregulated in YFES.•The improvement was related to the inhibition of SREBP-1c and Cpt1a expression levels
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Objectives: This study sought to verify bidirectionality by examining a bidirectional relationship of school life adaptation and life satisfaction with self-esteem as a mediating variable over ...time.Methods: Based on data from 2,245 elementary school students extracted from Year 1 (2019), Year 2 (2020), and Year 3 (2021) of the Multicultural Youth Panel Survey (MAPS 2nd), this adopted structural equation modeling method using the autoregressive cross-lagged modeling.Results: The study derived the following results. First, adaptation to school life at a previous point affected self-esteem at a later point, self-esteem at a previous point affected life satisfaction at a later point. In other words, school life adaptation in 4th grade had a positive effect on life satisfaction in 6th grade through selfesteem at 5th grade. Second, life satisfaction at a previous point affected self-esteem at a later point. Selfesteem at an earlier point had an effect on school life adaptation at a later point. That is, life satisfaction in 4th grade had a positive effect on school life adaptation in 6th grade through self-esteem at 5th grade.Conclusion: This study found a bidirectional relationship of school life adaptation and life satisfaction with self-esteem as a mediating variable over time.
This study investigated the effect of teacher academic support, teacher-student relationship, and teacher achievement pressure on maths class engagement via maths self-efficacy. This study examined ...the relationships among these variables through four structural equation models and identified the model with the best fit. Data of 6,927 students from three waves (longitudinal data from fifth- to seventh-graders) of the Korea Education Longitudinal Survey were analysed. Model comparisons indicated that the model with maths self-efficacy partially mediating the relationship between teacher academic support and class engagement, and fully mediating the relationship of class engagement with teacher-student relationship and teacher achievement pressure, had the best fit. Teacher academic support directly and indirectly (maths self-efficacy mediation) influenced student maths class engagement. Teacher-student relationship and teacher achievement pressure indirectly influenced student maths engagement through maths self-efficacy. Based on these results, this study has several implications for promoting mathematics education.
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BFBNIB, NUK, PILJ, SAZU, UL, UM, UPUK
Designing highly active Keggin-anion anchored on pz-based frameworks is desirable but still a huge challenge. Herein, three new POM-based inorganic–organic hybrid compounds {Ag5(pz)7}(BW12O40) (1), ...{Ag5(pz)7}(SiW12O40)(OH)·H2O (2), (Hpyr){Ag(pz)}2(PMo12O40) (3) (pz = pyrazine, pyr = pyrrole) have been fabricated via a hydrothermal synthesis method. All compounds were characterized by elemental analyses, infrared spectroscopy (IR), powder X-ray diffraction (PXRD), thermogravimetric (TG), scanning electron micrographs (SEM), and transmission electron microscope (TEM). Single-crystal X-ray diffraction displays that the configurations of compounds 1 and 2 are parallel. In compound 1, the BW12O405– is as 8-connected node incorporated into the orifice of the Ag-pz framework forming the glamorous POM-based metal organic frameworks (POMOFs). And the 3D POMOFs possess a kind of novel topology {42·54}2{44·512·68·73·9}{44·53·63}{44·55·6·74·9}{73}. There is a metal–organic nanotube structure constructed by four Ag-pz chains in compound 3. Then, the opened POMOFs consist of PMo12O403– clusters encircled in metal–organic nanotubes with the topology {34·46·54·6}2{34·48·58·64·74}. When used as electrode materials, as-prepared compounds 1, 2, and 3 have high specific capacitance of 1058, 986, and 1611 F g–1 at a current density of 2.16A g–1 and favorable cycling stability; after 1000 cycles, the retention rates of the capacitance are 90.3%, 94.5%, and 84.8% at 15.12 A g–1 in supercapacitors, respectively. Moreover, the ability of compounds 1–3 to degrade dyes is excellent under UV irradiation. The degradation rates of RhB are 94.89% for 1, 93.06% for 2, and 96.88% for 3 after 150 min, and the degradation rates of MB are 96.48% for 1, 94.28% for 2, and 98.58% for 3 after 90 min. These results illustrate that compounds 1–3 have potential applications in energy functional materials and organic pollutant degradation.
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IJS, KILJ, NUK, PNG, UL, UM
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Research On Eenergy Feedback Type AC Electronic Load Xie, Xinyao; Fan, Lingyu; Ning, Yifan ...
2023 International Seminar on Computer Science and Engineering Technology (SCSET),
2023-April
Conference Proceeding
The study of AC electronic load for student learning and power testing proposed an energy feedback type AC electronic load. Energy feedback single phase AC electronic load is a power electronic ...device which can simulate resistive load and inductive and capacitive load of any power factor and return the absorbed energy to the power grid. The main circuit topology adopts AC/DC/AC structure, the former load characteristic simulation unit adopts the current loop closed-loop control, the latter stage energy feedback unit adopts the double closed-loop grid-connected control algorithm, and the main circuit rectifier and inverter adopt the unipolar PWM modulation scheme.
Friend leukemia virus integration 1 (FLI1), an ETS transcription factor family member, acts as an oncogenic driver in hematological malignancies and promotes tumor growth in solid tumors. However, ...little is known about the mechanisms underlying the activation of this proto-oncogene in tumors.
Immunohistochemical staining showed that FLI1 is aberrantly overexpressed in advanced stage and metastatic breast cancers. Using a CRISPR Cas9-guided immunoprecipitation assay, we identify a circular RNA in the FLI1 promoter chromatin complex, consisting of FLI1 exons 4-2-3, referred to as FECR1.Overexpression of FECR1 enhances invasiveness of MDA-MB231 breast cancer cells. Notably, FECR1 utilizes a positive feedback mechanism to activate FLI1 by inducing DNA hypomethylation in CpG islands of the promoter. FECR1 binds to the FLI1 promoter in cis and recruits TET1, a demethylase that is actively involved in DNA demethylation. FECR1 also binds to and downregulates in trans DNMT1, a methyltransferase that is essential for the maintenance of DNA methylation.
These data suggest that FECR1 circular RNA acts as an upstream regulator to control breast cancer tumor growth by coordinating the regulation of DNA methylating and demethylating enzymes. Thus, FLI1 drives tumor metastasis not only through the canonical oncoprotein pathway, but also by using epigenetic mechanisms mediated by its exonic circular RNA.
Summary
The secondary cell wall is an important carbon sink in higher plants and its biosynthesis requires coordination of metabolic fluxes in the phenylpropanoid pathway. In Arabidopsis (Arabidopsis ...thaliana), MYB75 and the KNOX transcription factor KNAT7 form functional complexes to regulate secondary cell wall formation in the inflorescence stem. However, the molecular mechanism by which these transcription factors control different branches of the phenylpropanoid pathway remains poorly understood in woody species. We isolated an R2R3‐MYB transcription factor MYB6 from Populus tomentosa and determined that it was expressed predominately in young leaves. Overexpression of MYB6 in transgenic poplar upregulated flavonoid biosynthetic gene expression, resulting in significantly increased accumulation of anthocyanin and proanthocyanidins. MYB6‐overexpression plants showed reduced secondary cell wall deposition, accompanied by repressed expression of secondary cell wall biosynthetic genes. We further showed that MYB6 interacted physically with KNAT7 and formed functional complexes that acted to repress secondary cell wall development in poplar and Arabidopsis. The results provide an insight into the transcriptional mechanisms involved in the regulation of the metabolic fluxes between the flavonoid and lignin biosynthetic pathways in poplar.
Significance Statement
The poplar MYB6 transcription factor is involved in the positive regulation of anthocyanin and proanthocyanidin biosynthesis but the inhibition of secondary cell wall formation with KNAT7 repressor.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
The aberrantly upregulated Friend leukemia virus integration 1 (
) is closely correlated with the malignant phenotype of small cell lung cancer (SCLC). It is interesting to note that the CRISPR gene ...knockout by Cas9 gRNAs that target the
coding region and the posttranscriptional knockdown by shRNAs that target the 3' region of
mRNA yielded distinct antimetastasis effects in SCLC cells. This study attempts to examine if
exonic circular RNAs (FECR) function as a new malignant driver that determines the metastatic phenotype in SCLC.
The clinical relevance of FECRs was examined in 56 primary SCLC tissues and 50 non-small cell lung cancer (NSCLC) tissues. The prognostic value of FECRs was examined by measuring serum exosomal FECRs in a longitudinal cohort of patients with SCLC. The oncogenic activity of FECRs was investigated in both SCLC cell lines and animal xenograft studies. Finally, we explored the molecular mechanisms underlying these noncoding RNAs as a malignant driver.
Therapeutic comparison of CRISPR Cas9 knockout and shRNA knockdown of
identified FECRs as a new noncanonical malignant driver in SCLC. Using RNA FISH and quantitative PCR, we found that FECR1 (exons 4-2-3) and FECR2 (exons 5-2-3-4) were aberrantly upregulated in SCLC tissues (
< 0.0001), and was positively associated with lymph node metastasis (
< 0.01). Notably, serum exosomal FECR1 was associated with poor survival (
= 0.038) and clinical response to chemotherapy. Silencing of FECRs significantly inhibited the migration in two highly aggressive SCLC cell lines and reduced tumor metastasis
. Mechanistically, we uncovered that FECRs sequestered and subsequently inactivated tumor suppressor miR584-3p, leading to the activation of the Rho Associated Coiled-Coil Containing Protein Kinase 1 gene (ROCK1).
This study identifies
exonic circular RNAs as a new oncogenic driver that promotes tumor metastasis through the miR584-ROCK1 pathway. Importantly, serum exosomal FECR1 may serve as a promising biomarker to track disease progression of SCLC.
Proanthocyanidins (PAs) are major defense phenolic compounds in the leaves of poplar (Populus spp.) in response to abiotic and biotic stresses. Transcriptional regulation of PA biosynthetic genes by ...the MYB-basic helix–loop–helix (bHLH)-WD40 complexes in poplar is not still fully understood.
Here, an Arabidopsis TT2-like gene MYB115 was isolated from Populus tomentosa and characterized by various molecular, genetic and biochemical approaches.
MYB115 restored PA productions in the seed coat of the Arabidopsis tt2 mutant. Overexpression of MYB115 in poplar activated expression of PA biosynthetic genes, resulting in a significant increase in PA concentrations. By contrast, the CRISPR/Cas9-generated myb115 mutant exhibited reduced PA content and decreased expression of PA biosynthetic genes. MYB115 directly activated the promoters of PA-specific structural genes. MYB115 interacted with poplar TT8. Coexpression of MYB115, TT8 and poplar TTG1 significantly enhanced the expression of ANR1 and LAR3. Additionally, transgenic plants overexpressing MYB115 had increased resistance to the fungal pathogen Dothiorella gregaria, whereas myb115 mutant exhibited greater sensitivity compared with wild-type plants.
Our data provide insight into the regulatory mechanisms controlling PA biosynthesis by MYB115 in poplar, which could be effectively employed for metabolic engineering of PAs to improve resistance to fungal pathogens.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NMLJ, NUK, OILJ, PNG, SAZU, SBCE, SBMB, UL, UM, UPUK
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