Acid mine drainages are characterized by their low pH and the presence of dissolved toxic metallic species. Microorganisms survive in different microhabitats within the ecosystem, namely water, ...sediments, and biofilms. In this report, we surveyed the microbial diversity within all domains of life in the different microhabitats at Los Rueldos abandoned mercury underground mine (NW Spain), and predicted bacterial function based on community composition. Sediment samples contained higher proportions of soil bacteria (AD3, Acidobacteria), as well as Crenarchaeota and Methanomassiliicoccaceae archaea. Oxic and hypoxic biofilm samples were enriched in bacterial iron oxidizers from the genus
, order Acidithiobacillales, class Betaproteobacteria, and archaea from the class Thermoplasmata. Water samples were enriched in Cyanobacteria and Thermoplasmata archaea at a 3-98% of the sunlight influence, whilst Betaproteobacteria, Thermoplasmata archaea, and Micrarchaea dominated in acid water collected in total darkness. Stalactites hanging from the Fe-rich mine ceiling were dominated by the neutrophilic iron oxidizer
and other lineages that were absent in the rest of the microhabitats (e.g., Chlorobi, Chloroflexi). Eukaryotes were detected in biofilms and open-air water samples, and belonged mainly to clades SAR (Alveolata and Stramenopiles), and Opisthokonta (Fungi). Oxic and hypoxic biofilms displayed higher proportions of ciliates (
), whereas water samples were enriched in fungi (
and unknown microbial Helotiales). Predicted function through bacterial community composition suggested adaptive evolutive convergence of function in heterogeneous communities. Our study showcases a broad description of the microbial diversity across different microhabitats in the same environment and expands the knowledge on the diversity of microbial eukaryotes in AMD habitats.
The inorganic content of the well-preserved 3.2-m record of Las Conchas bog (NW Spain), covering 8000 cal yr BP., was analysed. To study natural vs. human contributions, we applied an innovative ...approach, namely the sequential study of multivariate statistics (factor analysis followed by clustering of the factor score matrix) and enrichment factors (EFs). The increasing weight of potentially toxic elements (PTEs) such as the geochemical association of Zn, Pb and Cd (EFs higher than 10, 20 and 40 in the last two centuries) was revealed, and corroborated by the contrast between the contents of anthropogenic Pb and total Rare Earth Elements (a suitable proxy for natural geogenic supplies). Furthermore, elements such as Hg, Tl and As also showed enrichment in the most recent samples of the study core. Some of them are commonly associated with global atmospheric transport; however, in this case, their increasing contents could also be explained by nearby industrial and mining activities.
In summary, severe pollution was observed in the uppermost part of the record, thereby pointing to an important environmental concern. Given that local and regional sources of PTEs, such as mining and heavy industry, especially Zn smelting, were probably the main historical causes of this contamination and that some of these industries are still active, we consider that our findings deserve further attention.
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•Las Conchas bog has a preserved inorganic record of more than 8000 years BP.•Multivariate statistics revealed a notable anthropic input in the last 225 years.•Cd–Zn–Pb and Hg-As high contents mainly related to regional mining & industry.
Capsule: Regional industrial and mining sources dramatically accelerated PTEs deposition in the last 225 years in NW Spain.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Identifying inorganic and organic soil contaminants in urban brownfields can give insights into the adverse effects of industrial activities on soil function, ecological health, and environmental ...quality. Liberty State Park in Jersey City (N.J., USA) once supported a major rail yard that had dock facilities for both cargo and passenger service; a portion remains closed to the public, and a forest developed and spread in this area. The objectives of this study were to: 1) characterize the organic and inorganic compounds in Liberty State Park soils and compare the findings to an uncontaminated reference site (Hutcheson Memorial Forest); and 2) identify differences between the barren low-functioning areas and the forested high-functioning areas of the brownfield. Soil samples were solvent-extracted, fractionated, and analyzed by gas chromatography–mass spectrometry and subjected to loss-on-ignition, pyrolysis-gas chromatography–mass spectrometry, inductively-coupled-plasma mass spectrometry, and optical microscopy analyses. Compared to soil from the reference site, the forested soils in Liberty State Park contained elevated percentages of organic matter (30–45%) and more contaminants, such as fossil-fuel-derived hydrocarbons and coal particles. Microscopy revealed bituminous and anthracite coal, coke, tar/pitch, and ash particles. Barren and low-functioning site 25R had a similar organic contaminant profile but contained a higher metal load than other Liberty State Park sites and also lacked higher plant indicators. These can obscure the signatures of contaminants, and data from adjacent barren and vegetated sites are valuable references for soils studies. A deeper understanding of the chemistry, biochemistry, and ecology of barren soils can be leveraged to prevent land degradation and to restore dysfunctional and phytotoxic soils.
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•We characterized organic and inorganic substances at a major urban brownfield site.•We detected abundant petroleum- and coal-derived biomarkers and PAHs in the soil.•Both barren low-functioning and forested soils contained similar organic compounds.•The barren site featured very high heavy metal loads and dormant microbial life.•Chemical and ecological studies are critical to brownfield restoration efforts.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
In the industrialized urban area of Avilés (Spain), pooled soil samples from grassland sites were taken after careful sampling design and then investigated for their content of trace elements. A ...combination of statistic and geostatistic techniques proved to be a reliable tool in the interpretation of the analytical results. Geochemical groups and areas influenced mainly by anthropogenic activities were distinguished by means of classic multivariate methods (factor analysis and cluster analysis) and innovative geostatistical tools (Fourier spectral analysis). The constitution of the groups is supported by the linked results of methods sequentially distributed in order to extract as much information as possible from the original data.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
•Low Mg/Ca and ash and high TOC not always linked strictly to ombrotrophic bogs.•Humid conditions between 8000 and 1000calyr BP were linked to geomorphology.•Bog conditions developed in the bottom ...20cm of the profile.•Good preservation of organic matter, except for upper 20cm.•Bacterial influence occurred from the bottom to 94cm.
We determined the lipid distributions (n-alkanes, n-alkan-2-ones, n-alkanoic acids), total organic carbon (TOC), total nitrogen (TN), Ca/Mg and ash content in Las Conchas mire, a 3.2m deep bryophyte-dominated mire in Northern Spain covering 8000calyr BP. Bog conditions developed in the bottom 20cm of the profile, and good preservation of organic matter (OM) was inferred from n-alkanoic acid distribution, with the exception of the uppermost 20cm (last ca. 200yr). Microbial synthesis of long chain saturated fatty acids from primary OM likely produced a dominance of short chain n-alkanoic acids with a bimodal distribution, as well as the lack of correspondence between the n-alkane and n-alkanoic acid profiles in the upper 20cm. This was accompanied by an increase in ash content, a decrease in TOC and variation in n-alkane ratios, thereby suggesting significant changes in the mire, namely drainage and transformation to a meadow, in the last ca. 200yr. The distribution of n-alkan-2-ones indicated an increase in bacterial source from the bottom of the record to 94cm, whereas their distribution in the upper part could be attributed mainly to plant input and/or the microbial oxidation of n-alkanes. The different n-alkane proxies showed variations, which we interpreted in terms of changes in vegetation (Sphagnum vs. non-Sphagnum dominated phases) during the last 8000calyr BP. C23 was the most abundant homolog throughout most of the record, thereby suggesting dominant humid conditions alternating with short drier phases. However, such humid conditions were not linked to paleoclimatic variation but rather to geomorphological characteristics: Las Conchas mire, at the base of the Cuera Range, receives continuous runoff—even during drier periods—which is not necessarily accompanied by additional mineral input to peat, producing the development of Sphagnum moss typical of waterlogged ecotopes and damp habitats. Thus, although geochemical proxies indicated an ombrotrophic regime in the mire, geomorphological characteristics may make a considerable contribution to environmental conditions.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
The development of a simple laboratory methodology allows the implementation of in situ bioremediation of polluted soils with diesel fuel. In this investigation microbiological and chemical analyses ...and a suitable bioreactor design, were very useful for suggesting the best ways to improve biodegradation extents in a diesel-enriched soil. Biostimulation with inorganic nitrogen and phosphorus produced the best results in a simple bioreactor, with biodegradation extents higher than 90% after 45 days. Also, the addition of activated sludge from a domestic wastewater plant increased the degradation rate to a great extent. In both cases, microbiological studies showed the presence of Acinetobacter sp. degrading most of the hydrocarbons. Simultaneously, a diesel fuel release (approximately 400,000 l) was studied. Samples taken in polluted soil and water revealed that bacteria from the genus Acinetobacter were predominant. In plate studies, Acinetobacter colonies produced a whitish substance with the characteristics of a biosurfactant. Remarkably, the presence of this product was evident at the field site, both in the riverbanks and in the physical recovery plant. The study of the similarities between laboratory results and the diesel spill site strongly suggested that natural conditions at the field site allowed the implementation of in situ bioremediation after physical removal of LNAPL (light nonaqueous-phase liquids).
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OBVAL, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
ABSTRACT Mapping Nearby Galaxies at Apache Point Observatory (MaNGA) is an optical fiber-bundle integral-field unit (IFU) spectroscopic survey that is one of three core programs in the ...fourth-generation Sloan Digital Sky Survey (SDSS-IV). With a spectral coverage of 3622-10354 and an average footprint of ∼500 arcsec2 per IFU the scientific data products derived from MaNGA will permit exploration of the internal structure of a statistically large sample of 10,000 low-redshift galaxies in unprecedented detail. Comprising 174 individually pluggable science and calibration IFUs with a near-constant data stream, MaNGA is expected to obtain ∼100 million raw-frame spectra and ∼10 million reduced galaxy spectra over the six-year lifetime of the survey. In this contribution, we describe the MaNGA Data Reduction Pipeline algorithms and centralized metadata framework that produce sky-subtracted spectrophotometrically calibrated spectra and rectified three-dimensional data cubes that combine individual dithered observations. For the 1390 galaxy data cubes released in Summer 2016 as part of SDSS-IV Data Release 13, we demonstrate that the MaNGA data have nearly Poisson-limited sky subtraction shortward of ∼8500 and reach a typical 10 limiting continuum surface brightness = 23.5 AB arcsec−2 in a five-arcsecond-diameter aperture in the g-band. The wavelength calibration of the MaNGA data is accurate to 5 km s−1 rms, with a median spatial resolution of 2.54 arcsec FWHM (1.8 kpc at the median redshift of 0.037) and a median spectral resolution of = 72 km s−1.
► Peat-forming plants other than
Sphagnum moss contain
n-alkan-2-ones. ► Almost all the species maximized at C
27 or C
29 ketones except ferns (C
21–C
23). ► Microbial degradation can be a major ...contributor to the ketones in sediments.
We determined the distribution of lipids (
n-alkanes and
n-alkan-2-ones) in present-day peat-forming plants in the Roñanzas Bog in northern Spain. Consistent with the observation of others, most
Sphagnum (moss) species alkanes maximized at C
23, whereas the other plants maximized at higher molecular weight (C
27 to C
31). We show for the first time that plants other than seagrass and
Sphagnum moss contain
n-alkan-2-ones. Almost all the species analysed showed an
n-alkan-2-one distribution between C
21 and C
31 with an odd/even predominance, maximizing at C
27 or C
29, except ferns, which maximized at lower molecular weight (C
21–C
23). We also observed that microbial degradation can be a major contributor to the
n-alkan-2-one distribution in sediments as opposed to a direct input of ketones from plants.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
Aging involves progressive physiological and metabolic reprogramming to adapt to gradual deterioration of organs and functions. This includes mechanisms of defense against pre-malignant ...transformations. Thus, certain tumors are more prone to appear in elderly patients. This is the case of the two most frequent types of primary liver cancer, i.e., hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (iCCA). Accordingly, aging hallmarks, such as genomic instability, telomere attrition, epigenetic alterations, altered proteostasis, mitochondrial dysfunction, cellular senescence, exhaustion of stem cell niches, impaired intracellular communication, and deregulated nutrient sensing can play an important role in liver carcinogenesis in the elders. In addition, increased liver fragility determines a worse response to risk factors, which more frequently affect the aged population. This, together with the difficulty to carry out an early detection of HCC and iCCA, accounts for the late diagnosis of these tumors, which usually occurs in patients with approximately 60 and 70 years, respectively. Furthermore, there has been a considerable controversy on what treatment should be used in the management of HCC and iCCA in elderly patients. The consensus reached by numerous studies that have investigated the feasibility and safety of different curative and palliative therapeutic approaches in elders with liver tumors is that advanced age itself is not a contraindication for specific treatments, although the frequent presence of comorbidities in these individuals should be taken into consideration for their management.
H i-MaNGA: H i follow-up for the MaNGA survey Masters, Karen L; Stark, David V; Pace, Zachary J ...
Monthly notices of the Royal Astronomical Society,
09/2019, Volume:
488, Issue:
3
Journal Article
Peer reviewed
Open access
Abstract
We present the H i-MaNGA programme of H i follow-up for the Mapping Nearby Galaxies at Apache Point Observatory (MaNGA) survey. MaNGA, which is part of the Fourth phase of the Sloan Digital ...Sky Surveys, is in the process of obtaining integral field unit spectroscopy for a sample of ∼10 000 nearby galaxies. We give an overview of the H i 21cm radio follow-up observing plans and progress and present data for the first 331 galaxies observed in the 2016 observing season at the Robert C. Bryd Green Bank Telescope. We also provide a cross-match of the current MaNGA (DR15) sample with publicly available H i data from the Arecibo Legacy Fast Arecibo L-band Feed Array survey. The addition of H i data to the MaNGA data set will strengthen the survey’s ability to address several of its key science goals that relate to the gas content of galaxies, while also increasing the legacy of this survey for all extragalactic science.
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