Phytoremediation is an effective way to remediate metal-contaminated soils. During phytoremediation, plants immobilize heavy metals through the roots to reduce the mobility, toxicity and dispersal of ...the metals, and the changes in the activity of the roots are often accompanied by changes in the rhizosphere ecosystems, in which rhizobacteria are essential components and interact with roots to maintain the stability of the rhizosphere ecosystem and improve soil health. In this study, the phytoremediation potential of Sasa argenteostriata (Regel) E.G. Camu and the response of rhizobacteria were revealed with different levels of lead-zinc tailing contamination (Pb, Zn, and Cd concentrations of 1197.53, 3243.40, and 185.44 mg/kg for M1 and 2301.71, 6087.95, and 364.00 mg/kg for M2, respectively). The BCF of Sasa argenteostriata increased with increasing soil pollution, and the BCFPb, BCFZn, and BCFCd were 0.19, 0.27, and 0.08, respectively, under the M2 treatment; in contrast, the TF decreased with increasing soil pollution, and the TFPb, TFZn, and TFCd were 0.39, 0.85, and 0.07, respectively, under the M1 treatment. The mobility of Pb in the rhizosphere was higher than that of Zn and Cd, and the percentage of residual (Res) Zn and Cd in the rhizosphere increased, while the acid-soluble (Aci) Pb was significantly higher, leading to obvious uptake of Pb by the roots. Correlation analysis showed that Sasa argenteostriata affected the rhizobacterial community by changing the rhizosphere soil pH, the contents of organic matter and NRFM, and bacteria such as Proteobacteria and MND1, which are highly resistant to heavy metals (HMs), became the dominant species in the community. Further PICRUSt2 analysis showed that reducing metal transport across the membranes and increasing the efficiency of cellular reproduction were the main metabolic mechanisms of bacterial tolerance to HMs. Overall, the roots of Sasa argenteostriata were able to immobilize more heavy metals in PbZn tailing-contaminated soil, reducing the toxicity of HMs in the soil, and then influencing the rhizobacteria to change the community structure and metabolism mechanism to adapt to the HM-contaminated environment, and the soil fertility was increased, which together promoted the health and stability of the soil. This study is the first to illustrate the phytoremediation potential and response of the rhizobacterial community of Sasa argenteostriata under multimetal contamination of PbZn tailings. The results of the study provide some guidance for the practice of lead-zinc tailing-phytoremediation and soil health.
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•BCF of S. argenteostriata increased with increasing pollution level, BCFZn>BCFPb>BCFCd, especially in roots.•TF of S. argenteostriata decreased with increasing pollution level, TFZn>TFPb>TFCd.•Soil organic matter increased significantly with increasing pollution level.•Bacteria like Burkholderiales are biomarkers with metal resistance in rhizosphere.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Indocalamus plants are low-growing shrubby bamboos with growth advantages, such as high biomass and strong resistance, and they are rich in germplasm resources in southern China. This study conducted ...soil lead (Pb) stress experiments on Indocalamus latifolius (Keng) McClure (LA), Indocalamus hunanensis B.M. Yang (HU), Indocalamus chishuiensis Y.L. Yang and Hsueh (CH) and Indocalamus lacunosus Wen (LC). Five Pb treatments (0, 500, 1000, 1500 mg·kg−1 Pb, and 1000 mg·kg−1 Pb + 1000 mg·kg−1 ethylenediamine tetraacetic acid (EDTA)) were established. EDTA was applied to explore the tolerance mechanism of different Indocalamus species after absorbing large amounts of heavy metals. The results were as follows: (1) under Pb treatment, the total relative biomass of LA, HU and LC was <100%, whereas the total relative biomass of CH was >100%; (2) after applying EDTA, the bioconcentration coefficient, translocation factor, and free proline content of the four Indocalamus species increased; and (3) the Pb mobility and distribution rates of the underground parts of the four Indocalamus species were consistently greater than those of the aboveground parts. The Pb mobility and distribution rates in the stems increased after applying EDTA, while those in the leaves decreased, as the plants tended to transfer Pb to their stems, which have lower physiological activity than their leaves.
Recent studies have shown that the combined application of ethylenediaminetetraacetic acid (EDTA) and degradable chelating agents can enhance EDTA's affinity for heavy metals and reduce its toxicity, ...but the effect of this combination on the phytoremediation remains largely unknown. This study evaluated and compared the effects of EDTA, nitrilotriacetic acid (NTA), and glutamic acid-N,N-diacetic acid (GLDA) alone (E, N, G treatment), and in combination (EN and EG treatment), on the growth of dwarf bamboo (
Q. H. Dai), their phytoremediation efficiency, and the soil environment in Pb-contaminated soil. The results showed that treatment E significantly reduced the biomass, while treatments N and EN were more conducive to the distribution of aerial plant biomass. Except for treatment E, the total Pb accumulation in all treatments increased significantly, with the highest increase in treatment EN. For double chelating agents, the acid-soluble Pb concentrations in rhizosphere and non-rhizosphere soils of treatments EN and EG were lower than those of treatment E, and the soil water-soluble Pb content after 20 days of treatment EN was significantly lower than that of treatment EG. Furthermore, chelating agents generally increased soil-enzyme activity in rhizosphere soil, indicating that chelating agents may promote plant heavy-metal uptake by changing the rhizosphere environment. In conclusion, treatment EN had the highest phytoremediation efficiency and significantly lower environmental risk than treatments E and EG, highlighting its massive potential for application in phytoremediation of Pb-contaminated soil when combined with
.
As a typical clonal plant, dwarf bamboo has potential applications in the remediation of Pb (lead) -contaminated soils; however, the environmental risk-spreading strategies of dwarf bamboo in ...heterogeneous Pb-contaminated environments are not known. To this end, we conducted hydroponic experiments on two species of groundcover bamboo, Sasa fortunei (Van Houtte) Fiori (SF) and Sasa auricoma (Mitford) E.G. Camus (SU), with seven treatments including two environments, homogeneous and heterogeneous. The results showed that SF protected the distal ramets (DRs) and that SU did the opposite in a homogeneous Pb environment. In the heterogeneous Pb environment, the NTR of both bamboos (RSISF = 0.64/0.44, RSISU = 0.40/0.33) was higher than that under the heterogeneous high-concentration environment (RSISF = 0.15/0.28, RSISU = 0.26/0.26). Moreover, the total Pb concentration of SF was as high as 80123.5 mg/kg and that of SU was as high as 53333.33 mg/kg, and in general, SF was more capable of absorbing Pb. In the heterogeneous low-Pb environment, the ascorbic acid and proline levels of the TRs (Pb-treated ramets) were elevated to alleviate oxidative stress; in contrast, antioxidant enzyme activities of SU were all reduced due to the damage caused by Pb absorption. The results of the experiments showed that SF had a greater risk-spreading capacity and higher Pb concentrations than SU in heterogeneous environments, and therefore, SF had better Pb bioenrichment and tolerance than SU. We proposed a novel metric (RSI) to assess the ability of plants to spread risk, and the results of the study provid a basis for dwarf bamboo phytoremediation in heterogeneous Pb environments.
•Adaptive strategies of dwarf bamboos in heterogeneous environments vary with concentration.•SF and SU has higher RSI in heterogeneous low concentrations, RSISF = 0.64/0.44, RSISU = 0.40/0.33.•The total Pb concentration of SF is higher than that of SU.•S. fortunei showed better risk-spreading capacity than S. auricoma.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Recent studies have shown that the combined application of ethylenediaminetetraacetic acid (EDTA) and degradable chelating agents can enhance EDTA’s affinity for heavy metals and reduce its toxicity, ...but the effect of this combination on the phytoremediation remains largely unknown. This study evaluated and compared the effects of EDTA, nitrilotriacetic acid (NTA), and glutamic acid-N,N-diacetic acid (GLDA) alone (E, N, G treatment), and in combination (EN and EG treatment), on the growth of dwarf bamboo (Indocalamus decorus Q. H. Dai), their phytoremediation efficiency, and the soil environment in Pb-contaminated soil. The results showed that treatment E significantly reduced the biomass, while treatments N and EN were more conducive to the distribution of aerial plant biomass. Except for treatment E, the total Pb accumulation in all treatments increased significantly, with the highest increase in treatment EN. For double chelating agents, the acid-soluble Pb concentrations in rhizosphere and non-rhizosphere soils of treatments EN and EG were lower than those of treatment E, and the soil water-soluble Pb content after 20 days of treatment EN was significantly lower than that of treatment EG. Furthermore, chelating agents generally increased soil-enzyme activity in rhizosphere soil, indicating that chelating agents may promote plant heavy-metal uptake by changing the rhizosphere environment. In conclusion, treatment EN had the highest phytoremediation efficiency and significantly lower environmental risk than treatments E and EG, highlighting its massive potential for application in phytoremediation of Pb-contaminated soil when combined with I. decorus.
The tumor microenvironment (TME) is connected to immunotherapy responses, but it remains unclear how cancer cells and host tissues differentially influence the immune composition within TME. Here, we ...performed single-cell analyses for autologous samples from liver metastasized colorectal cancer to disentangle factors shaping TME. By aligning CD45+ cells across different tissues, we classified exhausted CD8+ T cells (Texs) and activated regulatory T cells as M-type, whose phenotypes were associated with the malignancy, while natural killer and mucosal-associated invariant T cells were defined as N-type, whose phenotypes were associated with the niche. T cell receptor sharing between Texs in primary and metastatic tumors implicated the presence of common peripheral non-exhausted precursors. For myeloid cells, a subset of dendritic cells (DC3s) and SPP1+ macrophages were M-type, and the latter were predominant in liver metastasis, indicating its pro-metastasis role. Our analyses bridge immune phenotypes of primary and metastatic tumors, thereby helping to understand the tumor-specific contexture and identify the pro-metastasis components.
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•Immune cell phenotypic linkage with colorectal cancer and liver metastasis depicted•Malignancy-associated exhausted and regulatory T cells show diverse TCR dependency•SPP1+ TAMs are malignancy associated and are linked to liver metastasis•DCs are mainly associated with host organ except a malignancy-associated DC3 subset
Liu et al. reveal the roles of malignancy and host-organ contexture in shaping immune infiltrates within a tumor microenvironment based on single-cell phenotypic alignment of colorectal cancer and the autologous liver metastasis
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Abstract
System-wide metabolic homeostasis is crucial for maintaining physiological functions of living organisms. Stable-isotope tracing metabolomics allows to unravel metabolic activity ...quantitatively by measuring the isotopically labeled metabolites, but has been largely restricted by coverage. Delineating system-wide metabolic homeostasis at the whole-organism level remains challenging. Here, we develop a global isotope tracing metabolomics technology to measure labeled metabolites with a metabolome-wide coverage. Using
Drosophila
as an aging model organism, we probe the in vivo tracing kinetics with quantitative information on labeling patterns, extents and rates on a metabolome-wide scale. We curate a system-wide metabolic network to characterize metabolic homeostasis and disclose a system-wide loss of metabolic coordinations that impacts both intra- and inter-tissue metabolic homeostasis significantly during
Drosophila
aging. Importantly, we reveal an unappreciated metabolic diversion from glycolysis to serine metabolism and purine metabolism as
Drosophila
aging. The developed technology facilitates a system-level understanding of metabolic regulation in living organisms.
Background
The viral persistence in patients with Coronavirus Disease 2019 (COVID-19) remains to be investigated.
Methods
We investigated the viral loads, therapies, clinical features, and immune ...responses in a 70-year patient tested positive for SARS-CoV-2 for 3 months.
Findings
The patient exhibited the highest prevalence of abnormal indices of clinical features and immune responses at the first admission, including fever (38.3 ℃), decreased lymphocytes (0.83 × 10
9
/L) and serum potassium (3.1 mmol/L), as well as elevated serum creatinine (115 µmol/L), urea (8.6 mmol/L), and C-reactive protein (80 mg/L). By contrast, at the second and the third admission, these indices were all normal. Through three admissions, IL-2 increased from 0.14 pg/mL, 0.69 pg/mL, to 0.91 pg/mL, while IL-6 decreased from 11.78 pg/mL, 1.52 pg/mL, to 0.69 pg/mL, so did IL-10 from 5.13 pg/mL, 1.85 pg/mL, to 1.75 pg/mL. The steady declining trend was also found in TNF-α (1.49, 1.15, and 0.85 pg/mL) and IFN-γ (0.64, 0.42, and 0.27 pg/mL). The threshold cycle values of RT-PCR were 26.1, 30.5, and 23.5 for
ORFlab
gene, and 26.2, 30.6, and 22.7 for
N
gene, showing the patient had higher viral loads at the first and the third admission than during the middle term of the disease. The patient also showed substantially improved acute exudative lesions on the chest CT scanning images.
Conclusions
The patient displayed declining immune responses in spite of the viral shedding for 3 months. We inferred the declining immune responses might result from the segregation of the virus from the immune system.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, VSZLJ, ZAGLJ
Diabetes mellitus (DM) is one of the primary pathological factors that contributes to aging-related cognitive impairments, but the underlying mechanisms remain unclear. We recently reported that old ...DM rats exhibited impaired myogenic responses of the cerebral arteries and arterioles, poor cerebral blood flow autoregulation, enhanced blood-brain barrier (BBB) leakage, and cognitive impairments. These changes were associated with diminished vascular smooth muscle cell contractile capability linked to elevated reactive oxygen species (ROS) and reduced ATP production. In the present study, using a nonobese T2DN DM rat, we isolated parenchymal arterioles (PAs), cultured cerebral microvascular pericytes, and examined whether cerebrovascular pericyte in DM is damaged and whether pericyte dysfunction may play a role in the regulation of cerebral hemodynamics and BBB integrity. We found that ROS and mitochondrial superoxide production were elevated in PAs isolated from old DM rats and in high glucose (HG)-treated α-smooth muscle actin-positive pericytes. HG-treated pericytes displayed decreased contractile capability in association with diminished mitochondrial respiration and ATP production. Additionally, the expression of advanced glycation end products, transforming growth factor-β, vascular endothelial growth factor, and fibronectin were enhanced, but claudin 5 and integrin β1 was reduced in the brain of old DM rats and HG-treated pericytes. Further, endothelial tight junction and pericyte coverage on microvessels were reduced in the cortex of old DM rats. These results demonstrate our previous findings that the impaired cerebral hemodynamics and BBB leakage and cognitive impairments in the same old DM model are associated with hyperglycemia-induced cerebrovascular pericyte dysfunction.
This study demonstrates that the loss of contractile capability in pericytes in diabetes is associated with enhanced ROS and reduced ATP production. Enhanced advanced glycation end products (AGEs) in diabetes accompany with reduced pericyte and endothelial tight junction coverage in the cortical capillaries of old diabetic rats. These results suggest our previous findings that the impaired cerebral hemodynamics, BBB leakage, and cognitive impairments in old DM model are associated with hyperglycemia-induced cerebrovascular pericyte dysfunction.
In the present study, AIB1siRNA‑loaded polyethyleneimine (PEI)/heparin/Ca2+ nanoparticles (NPs) were successfully prepared and evaluated for their efficacy in lung cancer cells. The results ...demonstrated that the PEI and heparin complex reduced the toxic effect in cancer cells while maintaining its transfection efficiency. A nanosized particle of ~25 nm was formulated and siRNA was demonstrated to possess excellent binding efficiency in the particles. Confocal microscopy revealed that fluorescein‑labeled (FAM)‑small interfering (si)RNA dissociated from the HA‑PEI/heparin/Ca2+/siRNA (CPH‑siH) NPs and exhibited maximum fluorescence in the cytoplasm, which was important in elucidating its post‑transcriptional activity. CPH‑siH NPs exhibited a typical concentration‑dependent toxicity in cancer cells. Blank PEI/heparin/Ca2+ did not induce any toxicity in cancer cells, indicating its safety and lack of side effects. CPH‑siH (100 nm) induced the maximum apoptosis of cancer cells with nearly ~35% of cells in the early and late apoptosis stages. The expression of the nuclear receptor coactivator 3 (NCOA3, also known as AIB1) protein was knocked down in a concentration‑dependent manner, demonstrating the potent activity of AIB1siRNA in cancer cells. Together, these results indicated that HA‑PEI/heparin/Ca2+ NPs may be a promising carrier for the anticancer activity of AIB1siRNA in lung cancer cells.
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