Superparamagnetic iron oxide nanoparticles (SPION) with appropriate surface chemistry have been widely used experimentally for numerous in vivo applications such as magnetic resonance imaging ...contrast enhancement, tissue repair, immunoassay, detoxification of biological fluids, hyperthermia, drug delivery and in cell separation, etc. All these biomedical and bioengineering applications require that these nanoparticles have high magnetization values and size smaller than 100
nm with overall narrow particle size distribution, so that the particles have uniform physical and chemical properties. In addition, these applications need special surface coating of the magnetic particles, which has to be not only non-toxic and biocompatible but also allow a targetable delivery with particle localization in a specific area. To this end, most work in this field has been done in improving the biocompatibility of the materials, but only a few scientific investigations and developments have been carried out in improving the quality of magnetic particles, their size distribution, their shape and surface in addition to characterizing them to get a protocol for the quality control of these particles. Nature of surface coatings and their subsequent geometric arrangement on the nanoparticles determine not only the overall size of the colloid but also play a significant role in biokinetics and biodistribution of nanoparticles in the body. The types of specific coating, or derivatization, for these nanoparticles depend on the end application and should be chosen by keeping a particular application in mind, whether it be aimed at inflammation response or anti-cancer agents. Magnetic nanoparticles can bind to drugs, proteins, enzymes, antibodies, or nucleotides and can be directed to an organ, tissue, or tumour using an external magnetic field or can be heated in alternating magnetic fields for use in hyperthermia. This review discusses the synthetic chemistry, fluid stabilization and surface modification of superparamagnetic iron oxide nanoparticles, as well as their use for above biomedical applications.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
As per national policy, all diagnosed tuberculosis patients in India are to be tested using Xpert® MTB/RIF assay at the district level to diagnose rifampicin resistance. Regardless of the result, ...samples are transported to the reference laboratories for further testing: first-line Line Probe Assay (FL-LPA) for rifampicin-sensitive samples and second-line LPA(SL-LPA) for rifampicin-resistant samples. Based on the results, samples undergo culture and phenotypic drug susceptibility testing. We assessed among patients diagnosed with tuberculosis at 13 selected Xpert laboratories of Karnataka state, India, i) the proportion whose samples reached the reference laboratories and among them, proportion who completed the diagnostic algorithm ii) factors associated with non-reaching and non-completion and iii) the delays involved.
This was a cohort study involving review of programme records. For each TB patient diagnosed between 1st July and 31st August 2018 at the Xpert laboratory, we tracked the laboratory register at the linked reference laboratory until 30th September (censor date) using Nikshay ID (a unique patient identifier), phone number, name, age and sex.
Of 1660 TB patients, 1208(73%) samples reached the reference laboratories and among those reached, 1124(93%) completed the algorithm. Of 1590 rifampicin-sensitive samples, 1170(74%) reached and 1104(94%) completed the algorithm. Of 64 rifampicin-resistant samples, only 35(55%) reached and 17(49%) completed the algorithm. Samples from rifampicin-resistant TB, extra-pulmonary TB and two districts were less likely to reach the reference laboratory. Non-completion was more likely among rifampicin-resistant TB and sputum-negative samples. The median time for conducting and reporting results of Xpert® MTB/RIF was one day, of FL-LPA 5 days and of SL-LPA16 days.
These findings are encouraging given the complexity of the algorithm. High non-reaching and non-completion rates in rifampicin-resistant patients is a major concern. Future research should focus on understanding the reasons for the gaps identified using qualitative research methods.
Full text
Available for:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
With changing demographic patterns in the context of a high tuberculosis (TB) burden country, like India, there is very little information on the clinical and demographic factors associated with poor ...treatment outcome in the sub-group of older TB patients. The study aimed to assess the proportion of older TB patients (60 years of age and more), to compare the type of TB and treatment outcomes between older TB patients and other TB patients (less than 60 years of age) and to describe the demographic and clinical characteristics of older TB patients and assess any associations with TB treatment outcomes.
A retrospective cohort study involving a review of records from April to June 2011 in the 12 selected districts of Tamilnadu, India. Demographic, clinical and WHO defined disease classifications and treatment outcomes of all TB patients aged 60 years and above were extracted from TB registers maintained routinely by Revised National TB Control Program (RNTCP).
Older TB patients accounted for 14% of all TB patients, of whom 47% were new sputum positive. They had 38% higher risk of unfavourable treatment outcomes as compared to all other TB patients (Relative risk (RR)-1.4, 95% CI 1.2-1.6). Among older TB patients, the risk for unfavourable treatment outcomes was higher for those aged 70 years and more (RR 1.5, 95% CI 1.2-1.9), males (RR 1.5, 95% CI 1.0-2.1), re-treatment patients (RR 2.5, 95% CI 1.9-3.2) and those who received community-based Direct Observed Treatment (RR 1.4, 95% CI 1.1-1.9).
Treatment outcomes were poor in older TB patients warranting special attention to this group - including routine assessment and recording of co-morbidities, a dedicated recording, reporting and monitoring of outcomes for this age-group and collaboration with National programme of non-communicable diseases for comprehensive management of co-morbidities.
Full text
Available for:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
The aim of this study was to modify the surfaces of superparamagnetic iron oxide nanoparticles (SPION) with pullulan in order to reduce the cytotoxicity and enhance the cellular uptake of the ...nanoparticles. In this study, we have prepared and characterised the pullulan coated superparamagnetic iron oxide nanoparticles (Pn-SPION) of size around 40–45
nm with magnetite inner core and hydrophilic outer shell of pullulan. We have investigated the effect of cellular uptake of uncoated and Pn-SPION on cell adhesion/viability, cytotoxicity, morphology and cytoskeleton organisation of human fibroblasts. Cell cytotoxicity/adhesion studies of SPIONs on human dermal fibroblasts showed that the particles are toxic and their internalisation resulted in disruption of cytoskeleton organisation of cells. On the other hand, Pn-SPIONs were found to be non-toxic and induced changes in cytoskeleton organisation different from that observed with SPION. Transmission electron microscopy results indicated that the SPION and Pn-SPION were internalised into cells via different mechanisms, thereby suggesting that the particle endocytosis behaviour is dependent on the surface characteristics of the nanoparticles.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
Rising carbon emission or carbon footprint imposes grave concern over the earth׳s climatic condition, as it results in increasing average global temperature. Renewable energy sources seem to be the ...favorable solution in this regard. It can reduce the overall energy consumption rate globally. However, the renewable sources are intermittent in nature with very high initial installation price. Off-grid Small Autonomous Hybrid Power Systems (SAHPS) are good alternative for generating electricity locally in remote areas, where the transmission and distribution of electrical energy generated from conventional sources are otherwise complex, difficult and costly. In optimizing SAHPS, weather data over past several years are generally the main input, which include wind speed and solar radiation. The weather resources used in this optimization process have unsystematic variations based on the atmospheric and seasonal phenomenon and it also varies from year to year. While using past data in the analysis of SAHPS performance, it was assumed that the same pattern will be followed in the next year, which in reality is very unlikely to happen. In this paper, we use BBO optimization algorithm for SAHPS optimal component sizing by minimizing the cost of energy. We have also analysed the effect of using forecast weather data instead of past data on the SAHPS performance. ANNs, which are trained with back-propagation training algorithm, are used for wind speed and solar radiation forecasting. A case study was used for demonstrating the performance of BBO optimization algorithm along with forecasting effects. The simulation results clearly showed the advantages of utilizing wind speed and solar radiation forecasting in a SAHPS optimization problem.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
•Indicator-based vulnerability assessment demands correct identification of indicators.•Alpine forest of higher altitudes is less vulnerable than lower sub-alpine forests.•RS-GIS are useful tool for ...indicator mapping and were used successfully.
Understanding the vulnerability of forests and its associated factors is crucial for the sustainable management of forested landscapes. The assessment of vulnerability of forests in the Indian Western Himalayan (IWH) region comprising the states of Jammu & Kashmir (J&K), Himachal Pradesh (HP) and Uttarakhand (UK) was done using six indicators of vulnerability in the form of biological richness index, disturbance index, forest canopy density, fire point intensity and forest extraction intensity of fringe forests. We express this assessment as the “indicator-based vulnerability”. The indicators were allocated weights by multi criteria analysis using analytical hierarchy process. The spatial extent of all of the selected indicators was mapped for the IWH region at a pixel resolution of 24 m and was integrated to find out the vulnerability for each pixel in a GIS environment. The study area was divided into 172 grids of size 0.5°, equivalent to the grid size of available climatic projections, out of which 67 grids were identified as the forest grids. The grids that have at least 5% forest cover were designated as the forest grids and the vulnerability assessment was done only for these grids. The final representation of vulnerability across forested grids of the IWH was done at a spatial resolution of 5' and 0.5° to categorise as low, medium, high and very high class. It was observed that the highest concentration of “very high” and “high” vulnerable grids of 5' size lies in the state of UK, comprising 32 and 31%, respectively. The aggregated values at 0.5° indicate that most of the grids of UK fall under very high vulnerability except for the few uppermost and lowermost grids falling under other categories. In J&K, most of the 5' grids fall under low vulnerability (41%), while medium, high and very high categories are 27, 25 and 7%, respectively. Similarly, out of total 28 grids of size 0.5°, only one grid is categorized as very high vulnerable, while 11 grids fall under high vulnerability. In HP, none of the grids of either size is categorized as very high vulnerable. It was observed that most of the high and very high vulnerable grids in the IWH are in the lower altitudes while higher altitudes have lesser magnitude of vulnerability. Forests occurring at a higher elevation such as the Alpine forests (dry, moist and sub-alpine) is the least vulnerable forests compared to other forest type groups of the IWH.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
•Nature-based Solutions (NbS) in Himalayas has sustainable management and restoration of resources.•Most of the NbS of Himalaya adheres the IUCN standard criteria.•Improved NbS interventions in ...Himalaya requires precise planning and engaging multiple stakeholders.
Himalayan countries face many ecological challenges, including biodiversity loss and the many threats of climate change. In response, these countries are implementing Nature-based Solution to minimize these challenges and promote sustainable development. In this article we analyze the NbS programs implemented across the Himalayan region and assess the limitations of these NbS using the IUCN standard criteria of adherence to NbS for selected articles. We perform a systematic review of articles using the PRISMA methodology, through the Scopus, Google Scholar, and ResearchGate databases and followed by screening through the Rayyan web interface. Articles were screened out based on the location and lack of biodiversity and human well-being outcomes of NbS intervention. Nature-based Solutions in the Himalayas primarily use a sustainable management approach (35.29%), followed by restoration (23.53%), conservation (11.76%), holistic management (11.76%), eco-DRR (11.76%) and green infrastructure (5.88%) to address societal and climatic challenges. Institutional execution of NbS was 29.41% more common compared to the implementation of NbS by community or farmer groups. Pineapple based agroforestry (83.04%), forest restoration (78.57%), spring water storage (83.04%), spring restoration (86.61%), groundwater recharge (82.14%), bio-fertilizer and pesticides (77.68%), watershed restoration (78.57%), community-based watershed management (77.68%), community-based DRR (81.25%), community-based forest management (81.25%), sustainable harvest of medicinal plants (77.68%) and wetland restoration (82.14%), adhere to the IUCN criteria. In addition, some interventions adequately meet IUCN criteria, such as ice stupas (72.32%), forest restoration through plantation (73.21%), eco-safe roads (65.18), blue-green infrastructure (68.75%), and traditional ecological knowledge (72.32%). Our evaluation noted that NbS interventions can be improved by precise planning and design before implementation, engaging multiple stakeholders, comparing alternative solutions, and periodic monitoring of the impacts on the environment and society. This study shows that NbS in the Himalayas are contributing to biodiversity conservation and climate risk management. Tackling the limitation of this present review, mainly the exclusion of research not addressing biodiversity and human well-being, will yield more precise outcomes for climate actions and sustainable development.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Invasive plant species are considered one of the significant drivers of habitat loss, leading to biodiversity loss. They have also been observed to alter the local ecology, resulting in a decline of ...native flora. The management of invasive species is widely recognised as one of the most severe challenges to biodiversity conservation. The International Union for Conservation of Nature (IUCN) considers Lantana camara, as one of the ten worst weeds. Over time, native and indigenous species may evolve to co-exist or compete with invasive species, reducing invader fitness. It is observed that species competition fluctuates throughout environmental gradients, life phases, and abundances. Hence, competition outcome is very context-dependent. To address this challenge, we conducted a comprehensive study in three phases: we identified native species coexisting with Lantana in their natural habitats in the Doon Valley (Phase I) and documented the phenotypic traits of selected coexisting species using the Landmark BBCH (Biologische Bun-desantalt, Bundessortenamt und Chemische Industrie) scale, revealing the phenological growth patterns of selected co-existing species (Phase II). This was followed by conducting pot (Phase IIIa) and field (Phase IIIb) experiments to study the interactions between them. Notably, Justicia adhatoda, Broussonetia papyrifera, Pongamia pinnata, Urtica dioica and Bauhinia variegata demonstrated promising results in both pot and field conditions. Furthermore, after the mechanical removal of Lantana and prior to the plantation in the field experiments, four native grass species were introduced using the seed ball method. Among these, Pennisetum pedicellatum and Sorghum halpense exhibited prompt regeneration and effectively colonised the field, densely covering the cleared area. The study provides a comprehensive management plan for the restoration of Lantana affected areas through competition using native species. This study utilizes phenological assessment for native plant selection using reclamation from native grasses and proposes a management plan for combating invasive Lantana.
Full text
Available for:
IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
Axshya SAMVAD is an active tuberculosis (TB) case finding (ACF) strategy under project Axshya (Axshya meaning 'free of TB' and SAMVAD meaning 'conversation') among marginalized and vulnerable ...populations in 285 districts of India.
To compare patient characteristics, health seeking, delays in diagnosis and treatment initiation among new sputum smear positive TB patients detected through ACF and passive case finding (PCF) under the national TB programme in marginalized and vulnerable populations between March 2016 and February 2017.
This observational analytic study was conducted in 18 randomly sampled Axshya districts. We enrolled all TB patients detected through ACF and an equal number of randomly selected patients detected through PCF in the same settings. Data on patient characteristics, health seeking and delays were collected through record review and patient interviews (at their residence). Delays included patient level delay (from eligibility for sputum examination to first contact with any health care provider (HCP)), health system level diagnosis delay (from contact with first HCP to TB diagnosis) and treatment initiation delays (from diagnosis to treatment initiation). Total delay was the sum of patient level, health system level diagnosis delay and treatment initiation delays.
We included 234 ACF-diagnosed and 231 PCF-diagnosed patients. When compared to PCF, ACF patients were relatively older (≥65 years, 14% versus 8%, p = 0.041), had no formal education (57% versus 36%, p<0.001), had lower monthly income per capita (median 13.1 versus 15.7 USD, p = 0.014), were more likely from rural areas (92% versus 81%, p<0.002) and residing far away from the sputum microscopy centres (more than 15 km, 24% versus 18%, p = 0.126). Fewer patients had history of significant loss of weight (68% versus 78%, p = 0.011) and sputum grade of 3+ (15% versus 21%, p = 0.060). Compared to PCF, HCP visits among ACF patients was significantly lower (median one versus two HCPs, p<0.001). ACF patients had significantly lower health system level diagnosis delay (median five versus 19 days, p = 0.008) and the association remained significant after adjusting for potential confounders. Patient level and total delays were not significantly different.
Axshya SAMVAD linked the most impoverished communities to TB care and resulted in reduction of health system level diagnosis delay.
Full text
Available for:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
This study investigated the buckling behavior of Porous Functionally Graded Material (PFGM) plates. The present model assumes unevenly distributed porosity along the plate thickness and the use of ...the novel hyperbolic shear deformation functions and hyperbolic tangent and secant thickness stretching functions. In the present work, a porous Functionally Graded (FG) plate was analyzed by the principle of virtual work in order to understand the buckling behavior under uniaxial and biaxial compressive loading. The Rayleigh quotient method was applied to find the critical buckling load. The mesh convergence was investigated on a Finite Element (FE) model, and the accuracy of the results was compared with the prior research. The results of the proposed model match reasonably well with the ones of the published literature. Thorough parametric studies were performed to investigate the effect of porosity on the critical buckling load of the PFGM plate.
PDF
