This study aimed to evaluate the potentiality of a mineral and antioxidant-rich methanolic extract of the red marine alga
(FRE) against methyl-thiophanate (MT)-induced toxicity in adult rats. The ...animals were allocated into four groups: controls, MT (300 mg/kg), MT + FRE, and FRE-treated group for 7 days. Our results demonstrated severe mineral perturbations due to MT treatment, especially in calcium and phosphorus levels in plasma, urine, and bone. Similarly, the hematological analysis revealed increased red blood cells, platelets, and white blood cells associated with striking genotoxicity. Interestingly, a significant rise in lipid peroxidation and advanced oxidation protein products level in erythrocytes and bone were noted. Meanwhile, a depletion of the antioxidant status in both tissues occurred. These biochemical alterations were in harmony with DNA degradation and histological variation in bone and blood. In the other trend, data showed that treatment with alga improved MT-induced hematotoxicity, genotoxicity, and oxidative stress in the blood and bone. Osteo-mineral metabolism and bone histo-architecture were also noted. In conclusion, these findings demonstrated that the red alga
is a potent source of antioxidant and antibacterial agents, as revealed by the in vitro analysis.
Full text
Available for:
IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
This study aimed to evaluate the potentiality of a mineral and antioxidant-rich methanolic extract of the red marine alga Falkenbergia rufolanosa (FRE) against methyl-thiophanate (MT)-induced ...toxicity in adult rats. The animals were allocated into four groups: controls, MT (300 mg/kg), MT + FRE, and FRE-treated group for 7 days. Our results demonstrated severe mineral perturbations due to MT treatment, especially in calcium and phosphorus levels in plasma, urine, and bone. Similarly, the hematological analysis revealed increased red blood cells, platelets, and white blood cells associated with striking genotoxicity. Interestingly, a significant rise in lipid peroxidation and advanced oxidation protein products level in erythrocytes and bone were noted. Meanwhile, a depletion of the antioxidant status in both tissues occurred. These biochemical alterations were in harmony with DNA degradation and histological variation in bone and blood. In the other trend, data showed that treatment with alga improved MT-induced hematotoxicity, genotoxicity, and oxidative stress in the blood and bone. Osteo-mineral metabolism and bone histo-architecture were also noted. In conclusion, these findings demonstrated that the red alga Falkenbergia rufolanosa is a potent source of antioxidant and antibacterial agents, as revealed by the in vitro analysis.
Full text
Available for:
IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
A sulfated polysaccharide from tetrasporophyte tufts of Asparagopsis armata (Falkenbergia rufolanosa) (FRP) was extracted, then its structural, morphological and thermal properties were ...characterized. Antioxidant potential, enzymatic inhibitory effect and anticoagulant activities through activated partial thrombosis time, thrombin time and prothrombin time were also established. Additionally, this polymer was tested in vivo for its hepato-protective effect against toxicity induced by the fungicide — methyl thiophanate (MT). After MT injection, our data revealed a significant disruption in all biochemical and oxidative stress parameters associated with the hepatotoxicity features. Further, hepatic homogenates showed increased levels in inflammatory proteins consorted with detection of Bcl-2, IL-1beta, and P53 examined via immunohistochemistry revelation. These data were consolidate by liver histo-architecture and molecular investigation. However, the pre-treatment with FRP led to an effective healing process against MT's injuries. In summary, our data suggest that FRP could be assigned as a novel candidate for use as antioxidant, anticoagulant and hepato-protective agent.
•Polysaccharide (FRP) extracted from red marine alga Falkenbergia rufolanosa•FRP had good antioxidant activities in vitro.•FRP exhibited interesting enzymatic inhibitory effects.•Efficiency in prolonging clotting, typically for intrinsic and/or common pathways•Hepato-protective effect against toxicity induced by methyl thiophanate.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
PDF