In gynecologic oncology valid prognostic factors are necessary to estimate the course of disease and to define biologically similar subgroups for analysis of therapeutic efficacy. The presented study ...is a prospective study concerning prognostic significance of DNA ploidy and S-phase fraction in breast cancer following enrichment of tumor cells by cytokeratin labelling. Epithelial cells were labeled by FITC-conjugated cytokeratin antibody (CK 5, 6, 8, and CK 17) prior to flow cytometric cell cycle analysis in 327 fresh specimens of primary breast cancer. Univariate analysis in breast cancer detected the prognostic significance of DNA-ploidy, S-phase fraction and CV (coefficient of variation) of G(0)G(1)-peak of tumor cells for clinical outcome, especially for nodal-negative patients. Multivariate analysis could not confirm prognostic evidence of DNA-ploidy and S-phase fraction. In conclusion, in breast cancer no clinical significance for determination of DNA-parameters was found.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
Introduction: The distribution of histopathological features of invasive breast tumors in BRCA1 or BRCA2 germline mutation carriers differs from that of individuals with no known mutation. ...Histopathological features thus have utility for mutation prediction, including statistical modeling to assess pathogenicity of BRCA1 or BRCA2 variants of uncertain clinical significance. We analyzed large pathology datasets accrued by the Consortium of Investigators of Modifiers of BRCA1/2 (CIMBA) and the Breast Cancer Association Consortium (BCAC) to reassess histopathological predictors of BRCA1 and BRCA2 mutation status, and provide robust likelihood ratio (LR) estimates for statistical modeling. Methods: Selection criteria for study/center inclusion were estrogen receptor (ER) status or grade data available for invasive breast cancer diagnosed younger than 70 years. The dataset included 4, 477 BRCA1 mutation carriers, 2, 565 BRCA2 mutation carriers, and 47, 565 BCAC breast cancer cases. Country-stratified estimates of the likelihood of mutation status by histopathological markers were derived using a Mantel-Haenszel approach. Results: ER-positive phenotype negatively predicted BRCA1 mutation status, irrespective of grade (LRs from 0.08 to 0.90). ER-negative grade 3 histopathology was more predictive of positive BRCA1 mutation status in women 50 years or older (LR = 4.13 (3.70 to 4.62)) versus younger than 50 years (LR = 3.16 (2.96 to 3.37)). For BRCA2, ER-positive grade 3 phenotype modestly predicted positive mutation status irrespective of age (LR = 1.7-fold),whereas ER-negative grade 3 features modestly predicted positive mutation status at 50 years or older (LR = 1.54 (1.27 to 1.88)). Triple-negative tumor status was highly predictive of BRCA1 mutation status for women younger than 50 years (LR = 3.73 (3.43 to 4.05)) and 50 years or older (LR = 4.41 (3.86 to 5.04)),and modestly predictive of positive BRCA2 mutation status in women 50 years or older (LR = 1.79 (1.42 to 2.24)). Conclusions: These results refine likelihood-ratio estimates for predicting BRCA1 and BRCA2 mutation status by using commonly measured histopathological features. Age at diagnosis is an important variable for most analyses, and grade is more informative than ER status for BRCA2 mutation carrier prediction. The estimates will improve BRCA1 and BRCA2 variant classification and inform patient mutation testing and clinical management.
Malignant schwannomas are rare. These malignomas are primary nerve sheath tumors that usually arise from a peripheral nerve. They appear most frequently in the lower extremities, including the hips ...and buttocks (34.8%), the upper extremities (23.4%), and in the trunk (17%). To the best of our knowledge, this is the first reported case of a malignant schwannoma of the rectum. We describe the rare disease with reference to the literature.
We have built an infrared beam combiner for the GI2T/REGAIN interferometer of
the Observatoire de la Cote d'Azur. The beam combiner allows us to record
spectrally dispersed Michelson interference ...fringes in the near-infrared J-, H-
or K-bands. The beam combiner has the advantage that Michelson interferograms
can simultaneously be recorded in about 128 different spectral channels. The
tilt of the spectrally dispersed fringes is a measure of the instantaneous
optical path difference. We present the optical design of the beam combiner and
GI2T/REGAIN observations of the Mira star R Cas with this beam combiner in the
spectral range of 2.00 micron - 2.18 micron (observations on 22 and 25 August
1999; variability phase 0.08; V-magnitude approx. 6; seven baselines between
12m and 24m; reference stars Vega and Beta Peg). The spectrograph of the beam
combiner consists of an anamorphotic cylindrical lens system, an image plane
slit, and a grism. A system of digital signal processors calculates the
ensemble average power spectrum of the spectrally dispersed Michelson
interferograms and the instantaneous optical path difference error in real
time. From the observed R Cas visibilities at baselines 12.0m, 13.8m and 13.9m,
a 2.1 micron uniform-disk diameter of 25.3mas +/-3.3mas was derived. The
unusually high visibility values at baselines >16m show that the stellar
surface of R Cas is more complex than previously assumed. The visibility values
at baselines >16m can be explained by high-contrast surface structure on the
stellar surface of R Cas or other types of unexpected center-to-limb
variations. The R Cas observations were compared with theoretical Mira star
models yielding a linear Rosseland radius of 276Rsun +/-66Rsun and an effective
temperature of 2685K+/-238K for R Cas at phase 0.08.
We have built an infrared beam combiner for the GI2T/REGAIN interferometer of the Observatoire de la Cote d'Azur. The beam combiner allows us to record spectrally dispersed Michelson interference ...fringes in the near-infrared J-, H- or K-bands. The beam combiner has the advantage that Michelson interferograms can simultaneously be recorded in about 128 different spectral channels. The tilt of the spectrally dispersed fringes is a measure of the instantaneous optical path difference. We present the optical design of the beam combiner and GI2T/REGAIN observations of the Mira star R Cas with this beam combiner in the spectral range of 2.00 micron - 2.18 micron (observations on 22 and 25 August 1999; variability phase 0.08; V-magnitude approx. 6; seven baselines between 12m and 24m; reference stars Vega and Beta Peg). The spectrograph of the beam combiner consists of an anamorphotic cylindrical lens system, an image plane slit, and a grism. A system of digital signal processors calculates the ensemble average power spectrum of the spectrally dispersed Michelson interferograms and the instantaneous optical path difference error in real time. From the observed R Cas visibilities at baselines 12.0m, 13.8m and 13.9m, a 2.1 micron uniform-disk diameter of 25.3mas +/-3.3mas was derived. The unusually high visibility values at baselines >16m show that the stellar surface of R Cas is more complex than previously assumed. The visibility values at baselines >16m can be explained by high-contrast surface structure on the stellar surface of R Cas or other types of unexpected center-to-limb variations. The R Cas observations were compared with theoretical Mira star models yielding a linear Rosseland radius of 276Rsun +/-66Rsun and an effective temperature of 2685K+/-238K for R Cas at phase 0.08.
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